About: An insulated isothermal PCR method on a field-deployable device for rapid and sensitive detection of canine parvovirus type 2 at points of need

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About: An insulated isothermal PCR method on a field-deployable device for rapid and sensitive detection of canine parvovirus type 2 at points of need Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	An insulated isothermal PCR method on a field-deployable device for rapid and sensitive detection of canine parvovirus type 2 at points of need
Creator	Tsai, Chuan-Fu Tsai, Yun-Long Lee, Pei-Yu Chang, Hsiu-Hui Chang, Hsiao-Fen Wang, Hwa-Tang Wilkes, Rebecca
source	Elsevier; Medline; PMC
abstract	Canine parvovirus type 2 (CPV-2), including subtypes 2a, 2b and 2c, causes an acute enteric disease in both domestic and wild animals. Rapid and sensitive diagnosis aids effective disease management at points of need (PON). A commercially available, field-deployable and user-friendly system, designed with insulated isothermal PCR (iiPCR) technology, displays excellent sensitivity and specificity for nucleic acid detection. An iiPCR method was developed for on-site detection of all circulating CPV-2 strains. Limit of detection was determined using plasmid DNA. CPV-2a, 2b and 2c strains, a feline panleukopenia virus (FPV) strain, and nine canine pathogens were tested to evaluate assay specificity. Reaction sensitivity and performance were compared with an in-house real-time PCR using serial dilutions of a CPV-2b strain and 100 canine fecal clinical samples collected from 2010 to 2014, respectively. The 95% limit of detection of the iiPCR method was 13 copies of standard DNA and detection limits for CPV-2b DNA were equivalent for iiPCR and real-time PCR. The iiPCR reaction detected CPV-2a, 2b and 2c and FPV. Non-targeted pathogens were not detected. Test results of real-time PCR and iiPCR from 99 fecal samples agreed with each other, while one real-time PCR-positive sample tested negative by iiPCR. Therefore, excellent agreement (k = 0.98) with sensitivity of 98.41% and specificity of 100% in detecting CPV-2 in feces was found between the two methods. In conclusion, the iiPCR system has potential to serve as a useful tool for rapid and accurate PON, molecular detection of CPV-2.
has issue date	2015-04-15(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2015.04.007
bibo:pmid	25889355
has license	no-cc
sha1sum (hex)	c2ee01cd5dfb4c002d0b5d1f5d243f35f0cd00ee
schema:url	https://doi.org/10.1016/j.jviromet.2015.04.007
resource representing a document's title	An insulated isothermal PCR method on a field-deployable device for rapid and sensitive detection of canine parvovirus type 2 at points of need
has PubMed Central identifier	PMC7119629
has PubMed identifier	25889355
schema:publication	J Virol Methods
resource representing a document's body	covid:c2ee01cd5dfb4c002d0b5d1f5d243f35f0cd00ee#body_text
is schema:about of	named entity 'strains' named entity 'negative' named entity 'acute' named entity 'specificity' named entity 'system' named entity 'CPV' named entity 'FPV' named entity 'PCR' named entity 'pathogens' named entity 'domestic' named entity 'serial dilutions' named entity 'parvovirus' named entity 'RAPID' named entity 'TYPE 2' named entity 'NUCLEIC ACID DETECTION' named entity 'WILD ANIMALS' named entity 'SUBTYPES' named entity '100%' named entity 'CANINE PARVOVIRUS' named entity 'TOOL' named entity 'DETERMINED' named entity 'DIAGNOSIS AIDS' named entity 'PLASMID DNA' named entity 'FECES' named entity 'SENSITIVITY AND SPECIFICITY' named entity 'FPV' named entity 'ACCURATE' named entity 'SERIAL' named entity 'NON-' named entity 'PERFORMANCE' named entity 'DILUTIONS' named entity 'NOT DETECTED' named entity 'AVAILABLE' named entity 'EVALUATE' named entity 'FECAL' named entity 'CANINE' named entity 'USER' named entity 'EXCELLENT' named entity 'SAMPLES' named entity '100' named entity 'SYSTEM' named entity 'SAMPLE' named entity 'TESTED' named entity 'POSITIVE' named entity 'SENSITIVE' named entity 'CAUSES' named entity 'TARGETED' named entity 'STRAINS' named entity 'RAPID' named entity 'DISPLAYS' named entity 'PATHOGENS' named entity 'DNA' named entity 'POTENTIAL' named entity 'FRIENDLY' named entity 'TO SERVE AS' named entity 'TEST RESULTS' named entity 'POINTS' named entity 'FOUND' named entity 'ENTERIC' named entity 'CIRCULATING' named entity 'DOMESTIC' named entity 'REAL-TIME PCR ' named entity 'DISEASE' named entity 'PON' named entity 'DISEASE MANAGEMENT' named entity 'FELINE PANLEUKOPENIA VIRUS' named entity 'COPIES' named entity 'SENSITIVITY' named entity 'COLLECTED' named entity 'SITE' named entity 'LIMITS' named entity 'TECHNOLOGY' named entity 'DEVICE' named entity 'POINTS' named entity 'FIELD'

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