About: Synthetic Antibodies with a Human Framework That Protect Mice from Lethal Sudan Ebolavirus Challenge

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Synthetic Antibodies with a Human Framework That Protect Mice from Lethal Sudan Ebolavirus Challenge
Creator	Chen, Gang Chandran, Kartik Ye, Wei Dye, John Liu, Nina Long, Hua Nagar, Kaajal Pan, Guohua Frei, Julia Koellhoffer, Jayne Lai, Jonathan Sidhu, Sachdev Zak, Samantha
Source	Medline; PMC
abstract	[Image: see text] The ebolaviruses cause severe and rapidly progressing hemorrhagic fever. There are five ebolavirus species; although much is known about Zaire ebolavirus (EBOV) and its neutralization by antibodies, little is known about Sudan ebolavirus (SUDV), which is emerging with increasing frequency. Here we describe monoclonal antibodies containing a human framework that potently inhibit infection by SUDV and protect mice from lethal challenge. The murine antibody 16F6, which binds the SUDV envelope glycoprotein (GP), served as the starting point for design. Sequence and structural alignment revealed similarities between 16F6 and YADS1, a synthetic antibody with a humanized scaffold. A focused phage library was constructed and screened to impart 16F6-like recognition properties onto the YADS1 scaffold. A panel of 17 antibodies were characterized and found to have a range of neutralization potentials against a pseudotype virus infection model. Neutralization correlated with GP binding as determined by ELISA. Two of these clones, E10 and F4, potently inhibited authentic SUDV and conferred protection and memory immunity in mice from lethal SUDV challenge. E10 and F4 were further shown to bind to the same epitope on GP as 16F6 with comparable affinities. These antibodies represent strong immunotherapeutic candidates for treatment of SUDV infection.
has issue date	2014-08-20(xsd:dateTime)
bibo:doi	10.1021/cb5006454
bibo:pmid	25140871
has license	no-cc
sha1sum (hex)	c35f1a6432bd9d7f5bce38e59661f93b1aa13060
schema:url	https://doi.org/10.1021/cb5006454
resource representing a document's title	Synthetic Antibodies with a Human Framework That Protect Mice from Lethal Sudan Ebolavirus Challenge
has PubMed Central identifier	PMC4201348
has PubMed identifier	25140871
schema:publication	ACS Chem Biol
resource representing a document's body	covid:c35f1a6432bd9d7f5bce38e59661f93b1aa13060#body_text
is schema:about of	named entity 'HUMAN' named entity 'TERMS' named entity 'candidates' named entity 'Use' named entity 'USE' named entity 'HUMAN' named entity 'INFECTION' named entity 'IMMUNITY' named entity 'INCREASING' named entity 'CONTAINING' named entity 'RECOGNITION' named entity 'FOUND' named entity 'CLONES' named entity 'CAUSE' named entity 'CHALLENGE' named entity 'ELISA' named entity 'INHIBITED' named entity 'STARTING POINT' named entity 'POTENTIALS' covid:arg/c35f1a6432bd9d7f5bce38e59661f93b1aa13060 named entity 'LETHAL' named entity 'EBOLAVIRUS' named entity 'KNOWN' named entity 'PSEUDOTYPE VIRUS' named entity 'MUCH' named entity 'SEVERE' named entity 'SYNTHETIC' named entity 'STRONG' named entity 'PROGRESSING' named entity 'SPECIES' named entity 'ABOUT' named entity 'FREQUENCY' named entity 'LIKE' named entity 'ENVELOPE GLYCOPROTEIN' named entity 'MICE' named entity 'AFFINITIES' named entity 'FOCUSED' named entity 'EPITOPE' named entity 'BINDING' named entity 'ANTIBODIES' named entity 'ITS' named entity 'CONSTRUCTED' named entity 'TREATMENT' named entity 'STRUCTURAL ALIGNMENT' named entity 'INFECTION MODEL' named entity 'SUDAN EBOLAVIRUS' named entity 'REVEALED' named entity 'E10' named entity 'BINDS' named entity 'RANGE' named entity 'REPRESENT' named entity 'ANTIBODY' named entity 'PROTECTION' named entity 'SCAFFOLD' named entity 'RAPIDLY' named entity 'PHAGE LIBRARY' named entity 'CORRELATED' named entity 'SEQUENCE' named entity 'THESE' named entity 'PANEL' named entity 'CHARACTERIZED' named entity 'MICE' named entity 'CHALLENGE' named entity 'ANTIBODIES' named entity 'SUDAN EBOLAVIRUS' named entity 'LITTLE' named entity 'MONOCLONAL ANTIBODIES' named entity 'HUMANIZED' named entity 'MURINE'

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