About: Development of a recombinase polymerase amplification assay with lateral flow dipstick for rapid detection of feline parvovirus

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About: Development of a recombinase polymerase amplification assay with lateral flow dipstick for rapid detection of feline parvovirus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of a recombinase polymerase amplification assay with lateral flow dipstick for rapid detection of feline parvovirus
Creator	Wang, Xiao-Jia Wang, Zhao-Hua Hou, Shao-Hua
Source	Elsevier; Medline; PMC
abstract	Abstract Feline panleukopenia caused by feline parvovirus (FPV), a single-stranded DNA virus, is typically highly contagious and often presents with lethal syndrome. The broad spectrum of possible hosts suggests its potential for transmission from animal to person through close contact with pets. FPV thus serves as an example of the importance of new rapid point-of-care field diagnostic tools for the control and prevention of transmission, especially among rare wild animals and pet cats. Recombinase polymerase amplification (RPA), as a real-time and isothermal method, could be a more affordable alternative to PCR when combined with a lateral flow dipstick (LFD) indicator. In this study, we report a novel FPV lateral flow dipstick RPA (LFD-RPA) instant detection method capable of detecting a range of different FPV strains. The LFD-RPA assay consists of specific primers, probe, and nucleic acid strip. It is capable of detecting 102 copies of target nucleic acid per reaction, which is one order of magnitude higher than the sensitivity of traditional PCR. The most suitable reaction conditions for this assay are at 38 ℃ for 15 min. This paper develops an efficient visual detection system that can eliminate the need for professional staff and expensive and sophisticated equipment for field detection.
has issue date	2019-09-30(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2019.113679
bibo:pmid	31216435
has license	els-covid
sha1sum (hex)	c4073cf01e7461ddaca065207f42879ed953bb77
schema:url	https://doi.org/10.1016/j.jviromet.2019.113679
resource representing a document's title	Development of a recombinase polymerase amplification assay with lateral flow dipstick for rapid detection of feline parvovirus
has PubMed Central identifier	PMC7113848
has PubMed identifier	31216435
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:c4073cf01e7461ddaca065207f42879ed953bb77#body_text
is schema:about of	named entity 'HIGHLY' named entity 'ALTERNATIVE' named entity 'FELINE PARVOVIRUS' named entity 'INSTANT' named entity 'DIPSTICK' named entity 'PERSON' named entity 'REPORT' named entity 'TRADITIONAL' named entity 'PAPER' named entity 'METHOD' named entity 'PCR' named entity 'SYNDROME' named entity 'DIFFERENT' covid:arg/c4073cf01e7461ddaca065207f42879ed953bb77 named entity 'HOSTS' named entity 'DETECTION' named entity 'FELINE PANLEUKOPENIA' named entity 'NEED FOR' named entity 'LATERAL' named entity 'RAPID' named entity 'AMPLIFICATION' named entity 'FELINE PARVOVIRUS' named entity 'ASSAY' named entity 'POLYMERASE' named entity 'WILD ANIMALS' named entity 'AMPLIFICATION' named entity 'RANGE' named entity 'PET' named entity 'STRIP' named entity 'CAPABLE' named entity 'CONTACT WITH' named entity 'CONDITIONS' named entity 'DIAGNOSTIC' named entity 'DIPSTICK' named entity 'DEVELOPMENT' named entity 'RECOMBINASE' named entity 'SUITABLE' named entity 'ORDER' named entity 'ITS' named entity 'NUCLEIC ACID' named entity 'POINT-OF-CARE' named entity 'MIN' named entity 'CATS' named entity 'COPIES' named entity 'RPA' named entity 'PROFESSIONAL' named entity 'NOVEL' named entity 'VISUAL' named entity 'CAUSED BY' named entity 'TOOLS' named entity 'NEW' named entity 'FLOW' named entity 'REACTION' named entity 'POTENTIAL' named entity 'STRAINS' named entity 'FLOW' named entity 'CONTROL' named entity 'FIELD' named entity 'RECOMBINASE' named entity 'TARGET' named entity 'SYSTEM' named entity 'FPV' named entity 'BROAD SPECTRUM' named entity 'SENSITIVITY' named entity 'DETECTION METHOD' named entity 'SPECIFIC' named entity 'LETHAL' named entity 'MAGNITUDE' named entity 'TRANSMISSION' named entity 'RARE' named entity 'STUDY' named entity 'ASSAY' named entity 'STAFF' named entity 'DETECTION' named entity 'PRIMERS' named entity 'REAL-TIME' named entity 'RAPID' named entity 'POLYMERASE' named entity 'PETS'

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