About: Identification and characterization of vp7 gene in Bombyx mori cytoplasmic polyhedrosis virus

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About: Identification and characterization of vp7 gene in Bombyx mori cytoplasmic polyhedrosis virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Identification and characterization of vp7 gene in Bombyx mori cytoplasmic polyhedrosis virus
Creator	Chen, Fei Zhu, Min He, Lei Cao, Guangli Gong, Chengliang Hu, Xiaolong Liang, Zi Xue, Renyu Kuang, Sulan Zhu, Liyuan
Source	Elsevier; Medline; PMC
abstract	The genome of Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) contains 10 double stranded RNA segments (S1–S10). The segment 7 (S7) encodes 50 kDa protein which is considered as a structural protein. The expression pattern and function of p50 in the virus life cycle are still unclear. In this study, the viral structural protein 7 (VP7) polyclonal antibody was prepared with immunized mouse to explore the presence of small VP7 gene-encoded proteins in Bombyx mori cytoplasmic polyhedrosis virus. The expression pattern of vp7 gene was investigated by its overexpression in BmN cells. In addition to VP7, supplementary band was identified with western blotting technique. The virion, BmCPV infected cells and midguts were also examined using western blotting technique. 4, 2 and 5 bands were detected in the corresponding samples, respectively. The replication of BmCPV genome in the cultured cells and midgut of silkworm was decreased by reducing the expression level of vp7 gene using RNA interference. In immunoprecipitation experiments, using a polyclonal antiserum directed against the VP7, one additional shorter band in BmCPV infected midguts was detected, and then the band was analyzed with mass spectrum (MS), the MS results showed thatone candidate interacted protein (VP7 voltage-dependent anion-selective channel-like isoform, VDAC) was identified from silkworm. We concluded that the novel viral product was generated with a leaky scanning mechanism and the VDAC may be an interacted protein with VP7.
has issue date	2017-09-05(xsd:dateTime)
bibo:doi	10.1016/j.gene.2017.06.048
bibo:pmid	28668346
has license	no-cc
sha1sum (hex)	c4bca69f6cb21bb4b0214724a0e96abfd8b9027a
schema:url	https://doi.org/10.1016/j.gene.2017.06.048
resource representing a document's title	Identification and characterization of vp7 gene in Bombyx mori cytoplasmic polyhedrosis virus
has PubMed Central identifier	PMC7173298
has PubMed identifier	28668346
schema:publication	Gene
resource representing a document's body	covid:c4bca69f6cb21bb4b0214724a0e96abfd8b9027a#body_text
is schema:about of	named entity 'segments' named entity 'identified' named entity 'additional' named entity 'mass spectrum' named entity 'protein' named entity 'immunoprecipitation' named entity 'prepared' named entity 'double stranded RNA' covid:arg/c4bca69f6cb21bb4b0214724a0e96abfd8b9027a named entity 'addition' named entity 'VP7' named entity 'protein' named entity 'p50' named entity 'silkworm' named entity 'silkworm' named entity 'antiserum' named entity 'RNA' named entity 'cultured cells' named entity 'gene' named entity 'western blotting' named entity 'VP7' named entity 'virion' named entity 'infected' named entity 'VP7' named entity 'protein' named entity 'VP7' named entity 'kDa' named entity 'VDAC' named entity 'virion' named entity 'leaky scanning' named entity 'midgut' named entity 'anion' named entity 'cytoplasmic' named entity 'cytoplasmic' named entity 'pattern' named entity 'genome' named entity 'DLP' named entity 'VP7' named entity 'gene' named entity 'VP7' named entity 'gene' named entity 'fluorescein isothiocyanate' named entity 'Zeocin' named entity 'Zeocin' named entity 'apoptosis' named entity 'recombinant vector' named entity 'viral polymerase' named entity 'vp7' named entity 'VP7' named entity 'vp7' named entity 'viruses' named entity 'cultured cells' named entity 'kDa' named entity 'antibody' named entity 'ORFs' named entity 'gene' named entity 'silkworm' named entity 'Suda' named entity 'transfected' named entity 'silkworm' named entity 'proteolytic enzyme' named entity 'Bio-Rad' named entity 'siRNA' named entity 'vp1' named entity 'antibody' named entity 'Zeocin' named entity 'gene transcript' named entity 'ORF' named entity 'antibody' named entity 'Japan' named entity 'VP7' named entity 'BSA'

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