About: Screening of commercial meat products from supermarket chains for feline derivatives using SP-PCR-RLFP and lab-on-a-chip

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Screening of commercial meat products from supermarket chains for feline derivatives using SP-PCR-RLFP and lab-on-a-chip Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Screening of commercial meat products from supermarket chains for feline derivatives using SP-PCR-RLFP and lab-on-a-chip
Creator	Al Amin, Md Amin, Al Bcd, Rahman Chowdhury, Muhammad Desa, Desa, Mohd Hussain, Md Hussain, Mohd Msa, Razimi Nasir, Mohd Nasri, Md Nasri, Muhammad Rahman, Mahfujur Rahman, Mohd Razimi, Razimi, Ahmad Shahril, Ahmad Shahril, Mohd Zaira, Zaman Zaman Chowdhury, Zaira Zz, Chowdhury
Source	Elsevier; Medline; PMC
abstract	Determination of feline meat in food products is an important issue for social, health, economic and religious concern. Hence this paper documented the application of species specific polymerase chain reaction-restriction fragment length polymorphism (SP-PCR-RFLP) assay targeting a short-fragments (69 bp) of mitochondrial cytochrome b (cytb) gene to screen feline meat in commercial meat products using lab-on-a-chip. The SP-PCR assay proved its specificity theoretically and experimentally while testing with different common animal, aquatic and plant species of DNA. The feline specific (69 bp, 43- and 26-bp) characteristic molecular DNA pattern was observed by SP-PCR and RFLP analysis. For assay performance, it was tested in three different types of commercial dummy meat products such as frankfurters, nuggets and meatballs and digested with AluI-restriction enzyme. The highest sensitivity of the assay using lab-on-a-chip was as low as 0.1 pg or 0.01% (w/w) in commercial dummy meat products. We have also applied this assay to screen three important commercial meat products of six different brand from six supermarket chains located at three different states of Malaysia. Thus total 378 samples were tested to validate the specificity, sensitivity, stability of the assay and utilization of it for commercial meat product screening.
has issue date	2020-06-09(xsd:dateTime)
bibo:doi	10.1016/j.jfca.2020.103565
bibo:pmid	32546895
has license	no-cc
sha1sum (hex)	c56426d5c8654cfa7c7455279e9d7ca2ab8524eb
schema:url	https://doi.org/10.1016/j.jfca.2020.103565
resource representing a document's title	Screening of commercial meat products from supermarket chains for feline derivatives using SP-PCR-RLFP and lab-on-a-chip
has PubMed Central identifier	PMC7282760
has PubMed identifier	32546895
schema:publication	J Food Compost Anal
resource representing a document's body	covid:c56426d5c8654cfa7c7455279e9d7ca2ab8524eb#body_text
is schema:about of	named entity 'Screening' named entity 'Journal' named entity 'feline' named entity 'supermarket chains' named entity 'products' covid:arg/c56426d5c8654cfa7c7455279e9d7ca2ab8524eb named entity 'Screening' named entity 'MNM' named entity 'products' named entity 'meat products' named entity 'lab-on-a-chip' named entity 'meat products' named entity 'supermarket chains' named entity 'supermarket chains' named entity 'meat products' named entity 'lab-on-a-chip' named entity 'supermarket chains' named entity 'MEAT PRODUCTS' named entity 'LAB' named entity 'SP-' named entity 'assay' named entity 'amplicon' named entity 'SHORT' named entity 'PCR ASSAY' named entity 'DETECTION' named entity 'FELINE' named entity 'COMMERCIAL' named entity 'PCR' named entity 'PROOF' named entity 'SUPERMARKET' named entity 'SCREENING' named entity 'products' named entity 'assay' named entity 'feline' named entity 'feline' named entity 'Screening' named entity 'lab-on-a-chip' named entity 'FELINE' named entity 'USED' named entity 'lab-on-a-chip' named entity 'supermarket chains' named entity 'feline' named entity 'supermarket chains' named entity 'MEAT' named entity 'AMPLICON' named entity 'BASED' named entity 'PRE' named entity 'RLFP' named entity 'MNM' named entity 'USING' named entity 'SP-' named entity 'JOURNAL ' named entity 'LAB-ON-A-CHIP' named entity 'CHAINS' nodeID://b56697822 nodeID://b56781698 nodeID://b57059002 nodeID://b57506254 nodeID://b57564982
is wasQuotedFrom of	covid:arg/c56426d5c8654cfa7c7455279e9d7ca2ab8524eb/0 covid:arg/c56426d5c8654cfa7c7455279e9d7ca2ab8524eb/1

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software