About: Culture-Based Virus Isolation To Evaluate Potential Infectivity of Clinical Specimens Tested for COVID-19

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Culture-Based Virus Isolation To Evaluate Potential Infectivity of Clinical Specimens Tested for COVID-19 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Culture-Based Virus Isolation To Evaluate Potential Infectivity of Clinical Specimens Tested for COVID-19
Creator	Huang, Chung-Guei Hsiao, Mei-Jen Shih, Shin-Ru Tsao, Kuo-Chien Gong, Yu-Nong Yang, Shu-Li Huang, Po-Wei Lee, Kuo-Ming Liu, Yi-Chun Lin, Ya-Jhu Hsieh, Tzu-Hsuan Huang, Peng-Nien
Source	Medline; PMC
abstract	Real-time reverse transcription-PCR (RT-PCR) is currently the most sensitive method to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that causes coronavirus disease 2019 (COVID-19). However, the correlation between detectable viral RNA and culturable virus in clinical specimens remains unclear. Here, we performed virus culture for 60 specimens that were confirmed to be positive for SARS-CoV-2 RNA by real-time RT-PCR. The virus could be successfully isolated from 12 throat and nine nasopharyngeal swabs and two sputum specimens. The lowest copy number required for virus isolation was determined to be 5.4, 6.0, and 5.7 log(10) genome copies/ml sample for detecting the nsp12, E, and N genes, respectively. We further examined the correlation of genome copy number and virus isolation in different regions of the viral genome, demonstrating that culturable specimens are characterized by high copy numbers with a linear correlation observed between copy numbers of amplicons targeting structural and nonstructural regions. Overall, these results indicate that in addition to the copy number, the integrity of the viral genome should be considered when evaluating the infectivity of clinical SARS-CoV-2 specimens.
has issue date	2020-07-23(xsd:dateTime)
bibo:doi	10.1128/jcm.01068-20
bibo:pmid	32518072
has license	cc-by
sha1sum (hex)	c5cf851dd397b23bc08e70e5c97200ab2f82c923
schema:url	https://doi.org/10.1128/jcm.01068-20
resource representing a document's title	Culture-Based Virus Isolation To Evaluate Potential Infectivity of Clinical Specimens Tested for COVID-19
has PubMed Central identifier	PMC7383522
has PubMed identifier	32518072
schema:publication	J Clin Microbiol
resource representing a document's body	covid:c5cf851dd397b23bc08e70e5c97200ab2f82c923#body_text
is schema:about of	named entity 'virus' named entity 'copy number' named entity 'VIRAL RNA' named entity 'POSITIVE FOR' named entity 'CAUSES' named entity 'SWABS' named entity 'RESULTS' named entity 'INTEGRITY OF' named entity 'METHOD' named entity 'COULD BE' named entity 'COPY' named entity 'severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)' named entity 'specimens' named entity 'correlation' named entity 'integrity' named entity 'Overall' named entity 'SARS-CoV-2' named entity 'Viral culture' named entity 'Taiwan' named entity 'fetal bovine serum' named entity 'Genome' named entity 'severe acute respiratory syndrome coronavirus 2' named entity '5.7' named entity 'viral genome' named entity 'copy number' named entity 'genome' named entity 'virus' named entity 'sputum' named entity '5.4' named entity 'copy numbers' named entity 'RNA' named entity 'sampling bias' named entity 'symptom' named entity 'nonstructural' named entity 'SARS-CoV-2' named entity 'genome' named entity 'virus' named entity 'real-time RT-PCR' named entity 'RNA extraction' named entity 'genome' named entity 'transcriptome' named entity 'COVID' named entity 'GraphPad Prism' named entity 'nonstructural' named entity 'Taiwan' named entity 'SARS-CoV-2' named entity 'RT-PCR' named entity 'genome' named entity 'SARS-CoV-2' named entity 'freeze-thaw cycle' named entity 'nucleocapsid' named entity 'nucleic acid' named entity 'Thermo Fisher' named entity 'virus isolation' named entity 'plasmid' named entity 'virus' named entity 'freeze-thaw' named entity 'American Type Culture Collection' named entity 'SARS-CoV-2' named entity 'Eagle's medium' named entity 'Taiwan' named entity 'nucleocapsid' named entity 'Thermo Fisher Scientific' named entity 'nonstructural' named entity 'virus isolation' named entity 'inoculated' named entity 'RT-PCR' named entity 'virus' named entity 'freeze-thaw' named entity 'genome'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software