About: Use of the recombinant nonstructural 3A, 3B, and 3AB proteins of foot-and-mouth disease virus in indirect ELISA for differentiation of vaccinated and infected cattle

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About: Use of the recombinant nonstructural 3A, 3B, and 3AB proteins of foot-and-mouth disease virus in indirect ELISA for differentiation of vaccinated and infected cattle Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Use of the recombinant nonstructural 3A, 3B, and 3AB proteins of foot-and-mouth disease virus in indirect ELISA for differentiation of vaccinated and infected cattle
Creator	Mudrak, N Yakovleva, A Fomina, T Kan'shina, A Shcherbakov, A
source	Medline; PMC
abstract	Recombinant foot-and-mouth disease virus (FMDV) proteins 3A, 3B, and 3AB were produced by expressing the corresponding genes in Escherichia coli and purified by metal-chelate affinity chromatography. The recombinant proteins were used as antigens in indirect enzyme-linked immunosorbent assay (ELISA) to differentiate between vaccinated and FMD-infected animals. The following parameters were determined: working concentrations of antigens and peroxidase conjugate of cattle anti-IgG, the optimum composition of blocking buffer, and the positive-negative threshold of the reaction. Tests performed with approximately 200 serum samples taken from animals of different immunity states showed that the protocol with protein 3A as the antigen (3A-ELISA) provided the most reliable differentiation. All the newly developed systems proved to outperform the commercial Chekit FMD-3ABC kit in sensitivity, and 3A-ELISA was no less specific.
has issue date	2006-01-01(xsd:dateTime)
bibo:doi	10.1134/s0026893306010195
bibo:pmid	32214467
has license	no-cc
sha1sum (hex)	c5fb0f5f325c33b765df7115d1f00250c9b41f10
schema:url	https://doi.org/10.1134/s0026893306010195
resource representing a document's title	Use of the recombinant nonstructural 3A, 3B, and 3AB proteins of foot-and-mouth disease virus in indirect ELISA for differentiation of vaccinated and infected cattle
has PubMed Central identifier	PMC7089519
has PubMed identifier	32214467
schema:publication	Mol Biol
resource representing a document's body	covid:c5fb0f5f325c33b765df7115d1f00250c9b41f10#body_text
is schema:about of	named entity 'indirect' named entity 'determined' named entity 'peroxidase' named entity 'protein' named entity 'KIT' named entity 'INFECTED' named entity 'WORKING' named entity 'SERUM' named entity 'VACCINATED' named entity 'COMMERCIAL' named entity 'CONCENTRATIONS' named entity 'FMD' named entity 'RELIABLE' named entity 'NEGATIVE' named entity 'USED' named entity 'FOOT-AND-MOUTH DISEASE VIRUS' named entity 'PEROXIDASE' named entity 'serum' named entity 'composition' named entity 'protocol' named entity 'blocking' named entity 'proteins' named entity 'FMD' named entity 'antigens' named entity 'enzyme-linked immunosorbent assay' named entity 'FMD' named entity 'peroxidase' named entity 'chelate' named entity 'Moscow' named entity 'agarose' named entity '2.2' named entity 'Germany' named entity 'antibodies' named entity 'polymerase chain reaction' named entity 'artiodactyls' named entity 'imidazole' named entity 'recombinant proteins' named entity 'restriction sites' named entity 'nonstructural' named entity 'serum' named entity 'animal diseases' named entity 'protein' named entity 'pH 8' named entity 'serum' named entity 'Russia' named entity 'Promega' named entity 'E. coli' named entity 'Promega' named entity 'protein' named entity 'FMD' named entity 'recombinant proteins' named entity 'agarose' named entity 'low pH' named entity 'guanidine hydrochloride' named entity 'standard conditions' named entity 'FMDV' named entity 'RNA virus' named entity 'adsorbed' named entity 'antibodies' named entity 'chelate' named entity 'viral RNA' named entity 'guanidine' named entity 'Switzerland' named entity 'cattle' named entity 'plasmid' named entity 'Protein' named entity 'Cloning' named entity 'baculoviral' named entity 'chelate' named entity 'bovine coronavirus' named entity 'reverse transcriptase' named entity 'false positive results' named entity 'cattle' named entity 'Recombinant proteins' named entity 'virus' named entity '12,000' named entity 'nonstructural'

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