About: A novel enterovirus and parechovirus multiplex one-step real-time PCR-validation and clinical experience

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About: A novel enterovirus and parechovirus multiplex one-step real-time PCR-validation and clinical experience Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A novel enterovirus and parechovirus multiplex one-step real-time PCR-validation and clinical experience
Creator	Böttiger, Blenda Nielsen, Lars Christian, Alex Midgley, Sofie Nielsen, Yde
Source	Elsevier; Medline; PMC
abstract	As the number of new enteroviruses and human parechoviruses seems ever growing, the necessity for updated diagnostics is relevant. We have updated an enterovirus assay and combined it with a previously published assay for human parechovirus resulting in a multiplex one-step RT-PCR assay. The multiplex assay was validated by analysing the sensitivity and specificity of the assay compared to the respective monoplex assays, and a good concordance was found. Furthermore, the enterovirus assay was able to detect 42 reference strains from all 4 species, and an additional 9 genotypes during panel testing and routine usage. During 15 months of routine use, from October 2008 to December 2009, we received and analysed 2187 samples (stool samples, cerebrospinal fluids, blood samples, respiratory samples and autopsy samples) were tested, from 1546 patients and detected enteroviruses and parechoviruses in 171 (8%) and 66 (3%) of the samples, respectively. 180 of the positive samples could be genotyped by PCR and sequencing and the most common genotypes found were human parechovirus type 3, echovirus 9, enterovirus 71, Coxsackievirus A16, and echovirus 25. During 2009 in Denmark, both enterovirus and human parechovirus type 3 had a similar seasonal pattern with a peak during the summer and autumn. Human parechovirus type 3 was almost invariably found in children less than 4 months of age. In conclusion, a multiplex assay was developed allowing simultaneous detection of 2 viruses, which can cause similar clinical symptoms.
has issue date	2013-07-08(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2013.06.038
bibo:pmid	23845901
has license	no-cc
sha1sum (hex)	c60fd4c7e0d413db3e175610e2dfd8bbd38f5d27
schema:url	https://doi.org/10.1016/j.jviromet.2013.06.038
resource representing a document's title	A novel enterovirus and parechovirus multiplex one-step real-time PCR-validation and clinical experience
has PubMed Central identifier	PMC7119552
has PubMed identifier	23845901
schema:publication	J Virol Methods
resource representing a document's body	covid:c60fd4c7e0d413db3e175610e2dfd8bbd38f5d27#body_text
is schema:about of	named entity 'assay' named entity 'genotypes' named entity 'human' named entity 'one-step' named entity 'CLINICAL EXPERIENCE' named entity 'PARECHOVIRUS' named entity 'GENOTYPES' named entity 'PREVIOUSLY' named entity 'MONOPLEX' named entity 'GROWING' named entity 'ENTEROVIRUSES' named entity 'NOVEL' named entity 'DIAGNOSTICS' named entity 'COMPARED' named entity 'ADDITIONAL' named entity 'USAGE' named entity 'VALIDATED' named entity 'STEP' named entity 'VALIDATION' named entity 'STEP' named entity 'COMBINED' named entity 'REAL-TIME PCR' named entity 'MULTIPLEX' named entity 'HUMAN PARECHOVIRUSES' named entity 'ASSAY' named entity 'FOUND' named entity 'TESTING' named entity 'NEW' named entity 'ROUTINE' named entity 'RELEVANT' named entity 'PANEL' named entity 'HUMAN PARECHOVIRUS' named entity 'RT-PCR ASSAY' named entity 'REFERENCE' named entity 'SPECIES' named entity 'ENTEROVIRUS' named entity 'UPDATED' named entity 'MULTIPLEX ASSAY' named entity 'GOOD' named entity 'NECESSITY' named entity 'CONCORDANCE' named entity 'HAVE' named entity 'STRAINS' named entity 'PUBLISHED' named entity 'MULTIPLEX' named entity 'TO DETECT' named entity 'SENSITIVITY AND SPECIFICITY' named entity 'ENTEROVIRUS' named entity 'NUMBER OF' named entity 'RESULTING IN' covid:arg/c60fd4c7e0d413db3e175610e2dfd8bbd38f5d27 named entity 'human' named entity 'strains' named entity 'growing' named entity 'validated' named entity 'analysing' named entity 'enterovirus' named entity 'human parechovirus' named entity 'enterovirus' named entity 'real-time PCR' named entity 'combined' named entity 'EV71' named entity 'serial dilution' named entity 'Sint-Martens-Latem' named entity 'Primer' named entity 'multiplex assay' named entity 'MGB' named entity 'genotypes' named entity 'echovirus' named entity 'PV-1' named entity 'OC43' named entity 'poliovirus' named entity 'plasma' named entity 'WHO' named entity 'enterovirus' named entity 'GenBank' named entity 'fever'

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