About: The Amino-Terminal Region of Hepatitis E Virus ORF1 Containing a Methyltransferase (Met) and a Papain-Like Cysteine Protease (PCP) Domain Counteracts Type I Interferon Response

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About: The Amino-Terminal Region of Hepatitis E Virus ORF1 Containing a Methyltransferase (Met) and a Papain-Like Cysteine Protease (PCP) Domain Counteracts Type I Interferon Response Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	The Amino-Terminal Region of Hepatitis E Virus ORF1 Containing a Methyltransferase (Met) and a Papain-Like Cysteine Protease (PCP) Domain Counteracts Type I Interferon Response
Creator	Pavio, Nicole Doceul, Virginie Jouvenet, Nolwenn Bagdassarian, Eugénie Demange, Antonin Meyer, Léa Pellerin, Marie
source	PMC
abstract	Hepatitis E virus (HEV) is responsible for large waterborne epidemics of hepatitis in endemic countries and is an emerging zoonotic pathogen worldwide. In endemic regions, HEV-1 or HEV-2 genotypes are frequently associated with fulminant hepatitis in pregnant women, while with zoonotic HEV (HEV-3 and HEV-4), chronic cases of hepatitis and severe neurological disorders are reported. Hence, it is important to characterize the interactions between HEV and its host. Here, we investigated the ability of the nonstructural polyprotein encoded by the first open reading frame (ORF1) of HEV to modulate the host early antiviral response and, in particular, the type I interferon (IFN-I) system. We found that the amino-terminal region of HEV-3 ORF1 (MetYPCP), containing a putative methyltransferase (Met) and a papain-like cysteine protease (PCP) functional domain, inhibited IFN-stimulated response element (ISRE) promoter activation and the expression of several IFN-stimulated genes (ISGs) in response to IFN-I. We showed that the MetYPCP domain interfered with the Janus kinase (JAK)/signal transducer and activator of the transcription protein (STAT) signalling pathway by inhibiting STAT1 nuclear translocation and phosphorylation after IFN-I treatment. In contrast, MetYPCP had no effect on STAT2 phosphorylation and a limited impact on the activation of the JAK/STAT pathway after IFN-II stimulation. This inhibitory function seemed to be genotype-dependent, as MetYPCP from HEV-1 had no significant effect on the JAK/STAT pathway. Overall, this study provides evidence that the predicted MetYPCP domain of HEV ORF1 antagonises STAT1 activation to modulate the IFN response.
has issue date	2018-12-18(xsd:dateTime)
bibo:doi	10.3390/v10120726
bibo:pmid	30567349
has license	cc-by
sha1sum (hex)	c7ddb36588a83decb4c6bcd033f9a0b208e66973
schema:url	https://doi.org/10.3390/v10120726
resource representing a document's title	The Amino-Terminal Region of Hepatitis E Virus ORF1 Containing a Methyltransferase (Met) and a Papain-Like Cysteine Protease (PCP) Domain Counteracts Type I Interferon Response
has PubMed Central identifier	PMC6315852
has PubMed identifier	30567349
schema:publication	Viruses
resource representing a document's body	covid:c7ddb36588a83decb4c6bcd033f9a0b208e66973#body_text
is schema:about of	covid:arg/c7ddb36588a83decb4c6bcd033f9a0b208e66973 named entity 'open reading frame' named entity 'IFN' named entity 'HEV' named entity 'protein' named entity 'HEV' named entity 'pathogen' named entity 'IFN' named entity 'interferon' named entity 'hepatitis' named entity 'Janus kinase' named entity 'endemic' named entity 'methyltransferase' named entity 'response element' named entity 'interferon' named entity 'IFN' named entity 'signalling pathway' named entity 'ORF1' named entity 'HEV' named entity 'epidemics' named entity 'HEV' named entity 'pregnant women' named entity 'zoonotic' named entity 'Type I Interferon' named entity 'PCP' named entity 'Cysteine Protease' named entity 'PCP' named entity 'transfected' named entity 'ORF1' named entity 'HEV' named entity 'PBS' named entity 'zinc-finger' named entity 'IFN-β' named entity 'ISRE' named entity 'HEV' named entity 'STAT1' named entity 'phosphorylation' named entity 'IFN-β' named entity 'IFN-β' named entity 'PCP' named entity 'actin' named entity 'STAT1' named entity 'immunoblotting' named entity 'ORF1' named entity 'luciferase' named entity 'PCP' named entity 'Invitrogen' named entity 'actin' named entity 'transfected' named entity 'IFN' named entity 'RNA' named entity 'IFN-β' named entity 'Thermo Fisher' named entity 'Tyr' named entity 'phosphorylation' named entity 'primary antibodies' named entity 'digestion' named entity 'HEV' named entity 'IFN-β' named entity 'measles virus' named entity 'actin' named entity 'type II IFN' named entity '293T cells' named entity 'STAT1' named entity 'ISRE' named entity 'PCP' named entity 'Shiga' named entity 'pUC19' named entity 'virus' named entity 'serum' named entity 'Renilla luciferase' named entity 'STAT1' named entity 'SDS-PAGE' named entity 'HEV' named entity 'plasmid'

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