About: Highly Efficient Base Editing in Viral Genome Based on Bacterial Artificial Chromosome Using a Cas9-Cytidine Deaminase Fused Protein

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About: Highly Efficient Base Editing in Viral Genome Based on Bacterial Artificial Chromosome Using a Cas9-Cytidine Deaminase Fused Protein Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Highly Efficient Base Editing in Viral Genome Based on Bacterial Artificial Chromosome Using a Cas9-Cytidine Deaminase Fused Protein
Creator	Wang, Xin Chen, Huan-Chun Chen, Yu-Xin Jiang, • Fang-Fang Le Su, • Liu, Zheng-Fei Zheng, Ke
Source	PMC
abstract	Viruses evolve rapidly and continuously threaten animal health and economy, posing a great demand for rapid and efficient genome editing technologies to study virulence mechanism and develop effective vaccine. We present a highly efficient viral genome manipulation method using CRISPR-guided cytidine deaminase. We cloned pseudorabies virus genome into bacterial artificial chromosome, and used CRISPR-guided cytidine deaminase to directly convert cytidine (C) to uridine (U) to induce premature stop mutagenesis in viral genes. The editing efficiencies were 100%. Comprehensive bioinformatic analysis revealed that a large number of editable sites exist in pseudorabies virus (PRV) genomes. Notably, in our study viral genome exists as a plasmid in E. coli, suggesting that this method is virus species-independent. This application of base-editing provided an alternative approach to generate mutant virus and might accelerate study on virulence and vaccine development. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s12250-019-00175-4) contains supplementary material, which is available to authorized users.
has issue date	2019-12-02(xsd:dateTime)
bibo:doi	10.1007/s12250-019-00175-4
bibo:pmid	31792738
has license	no-cc
sha1sum (hex)	c7fd457fc1c7962364e672fa16b936076e759a59
schema:url	https://doi.org/10.1007/s12250-019-00175-4
resource representing a document's title	Highly Efficient Base Editing in Viral Genome Based on Bacterial Artificial Chromosome Using a Cas9-Cytidine Deaminase Fused Protein
has PubMed Central identifier	PMC7198655
has PubMed identifier	31792738
schema:publication	Virol Sin
resource representing a document's body	covid:c7fd457fc1c7962364e672fa16b936076e759a59#body_text
is schema:about of	named entity 'cytidine' named entity 'Fused' named entity 'Editing' named entity 'Efficient' named entity 'GENOME EDITING' named entity 'E. COLI' named entity 'BASE' named entity 'BIOINFORMATIC' named entity 'TECHNOLOGIES' named entity 'genome' named entity 'PRV' named entity 'continuously' named entity 'revealed' named entity 'bacterial artificial chromosome' named entity 'bioinformatic analysis' named entity 'virulence' named entity 'CRISPR' named entity 'cytidine' named entity 'cytidine deaminase' named entity 'PRV' named entity 'uridine' named entity 'virulence' named entity 'Cas9' named entity 'Viral Genome' named entity 'cytidine deaminase' named entity 'protein' named entity 'Viral titers' named entity 'sequenced' named entity 'codon' named entity 'Viruses' named entity 'infection' named entity 'LB medium' named entity 'mutagenesis' named entity 'genome' named entity 'plaque purification' named entity 'genetic stability' named entity 'genome' named entity 'PRV' named entity 'target site' named entity 'recombination' named entity 'titer' named entity 'genomes' named entity 'virus' named entity 'E. coli' named entity 'virology' named entity 'Gln' named entity 'plasmid' named entity 'PCR amplified' named entity 'Cas9' named entity 'agar plate' named entity 'BAC' named entity 'gene' named entity 'E. coli' named entity 'PRV' named entity 'recombinant' named entity 'herpesvirus' named entity 'virus' named entity 'genome' named entity 'multiplicity of infection' named entity 'intergenic region' named entity 'crystal violet' named entity 'antibiotics' named entity 'genome editing' named entity 'plasmid' named entity 'codons' named entity 'primers' named entity 'point mutations' named entity 'PRV' named entity 'E. coli' named entity 'genome' named entity 'eGFP' named entity 'swine' named entity 'SOC medium' named entity 'chloramphenicol' named entity 'cloned'

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