About: Leukocyte function assessed via serial microlitre sampling of peripheral blood from sepsis patients correlates with disease severity

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About: Leukocyte function assessed via serial microlitre sampling of peripheral blood from sepsis patients correlates with disease severity Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Leukocyte function assessed via serial microlitre sampling of peripheral blood from sepsis patients correlates with disease severity
Creator	Higuera, Angelica Pinilla-Vera, Mayra Baron, Rebecca Krishnamoorthy, Nandini Abdulnour, Raja-Elie Benson, Maura Duvall, Melody Engstrom, Braden Han, Jongyoon Jundi, Bakr Lee, Do-Hyun Lee, Jaemyon Levy, Bruce Ryu, Hyunryul Voldman, Joel
Source	PMC
abstract	Dysregulated leukocyte responses underlie the pathobiology of sepsis, which is a leading cause of death. However, measures of leukocyte function are not routinely available in clinical care. Here we report the development and testing of an inertial microfluidic system for the label-free isolation and downstream functional assessment of leukocytes from 50 μl of peripheral blood. We used the system to assess leukocyte phenotype and function in serial samples from 18 hospitalized patients with sepsis and 10 healthy subjects. The sepsis samples had significantly higher levels of CD16(dim) and CD16(−) neutrophils and CD16(+) ‘intermediate’ monocytes, as well as significantly lower levels of neutrophil-elastase release, O(2)(−) production and phagolysosome formation. Repeated sampling of sepsis patients over 7 days showed that leukocyte activation (measured by isodielectric separation) and leukocyte phenotype and function were significantly more predictive of the clinical course than complete-blood-count parameters. We conclude that the serial assessment of leukocyte function in microlitre blood volumes is feasible and that it provides significantly more prognostic information than leukocyte counting.
has issue date	2019-11-11(xsd:dateTime)
bibo:doi	10.1038/s41551-019-0473-5
bibo:pmid	31712645
has license	no-cc
sha1sum (hex)	c836e422a09d3e536679f468bc0dc6186e58335b
schema:url	https://doi.org/10.1038/s41551-019-0473-5
resource representing a document's title	Leukocyte function assessed via serial microlitre sampling of peripheral blood from sepsis patients correlates with disease severity
has PubMed Central identifier	PMC6899180
has PubMed identifier	31712645
schema:publication	Nat Biomed Eng
resource representing a document's body	covid:c836e422a09d3e536679f468bc0dc6186e58335b#body_text
is schema:about of	named entity 'intermediate' named entity 'function' named entity 'sampling' named entity 'samples' named entity 'monocytes' named entity 'separation' named entity 'microlitre' named entity 'responses' named entity 'function' named entity 'patients' named entity 'sepsis' named entity 'phenotype' named entity 'samples' named entity 'predictive' named entity 'leukocytes' named entity 'CD16' named entity 'sepsis' named entity 'phenotype' named entity 'leukocyte' named entity 'microlitre' named entity 'leukocyte' named entity 'leukocyte' named entity 'neutrophil' named entity 'sepsis' named entity 'peripheral blood' named entity 'leukocytes' named entity 'ANOVA' named entity 'sepsis' named entity 'CD16' named entity 'peripheral blood' named entity 'pathobiology' named entity 'PMNs' named entity 'pathobiology' named entity 'CD14' named entity 'PMNs' named entity 'flow cytometry' named entity 'IDP' named entity 'microfluidics' named entity 'microfluidics' named entity 'elastase' named entity 'leukocyte activation' named entity 'ice water' named entity 'operation time' named entity 'syringe pumps' named entity 'flow resistance' named entity 'clinical outcomes' named entity 'biomarkers' named entity 'leukocyte activation' named entity 'cell adhesion molecules' named entity 'CBC' named entity 'sepsis' named entity 'leukocyte' named entity 'intensive care unit' named entity '7.5' named entity 'superoxide anion' named entity 'sepsis' named entity 'PMNs' named entity 'CD42b' named entity 'CBC' named entity 'Physiology' named entity 'sepsis' named entity 'supernatant' named entity 'plasma' named entity 'PMNs' named entity 'sepsis' named entity 'mesenchymal stem cells' named entity 'Chronic Health'

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