About: Processing tissue and cells for transmission electron microscopy in diagnostic pathology and research

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Processing tissue and cells for transmission electron microscopy in diagnostic pathology and research
Creator	Orenstein, Jan Graham, Lesley
Source	Medline; PMC
abstract	In transmission electron microscopy (TEM), electrons are transmitted through a plastic-embedded specimen, and an image is formed. TEM enables the resolution and visualization of detail not apparent via light microscopy, even when combined with immunohistochemical analysis. Ultrastructural examination of tissues, cells and microorganisms plays a vital role in diagnostic pathology and biologic research. TEM is used to study the morphology of cells and their organelles, and in the identification and characterization of viruses, bacteria, protozoa and fungi. In this protocol, we present a TEM method for preparing specimens obtained in clinical or research settings, discussing the particular requirements for tissue and cell preparation and analysis, the need for rapid fixation and the possibility of analysis of tissue already fixed in formalin or processed into paraffin blocks. Details of fixation, embedding and how to prepare thin and semi-thin sections, which can be used for analysis complementary to that performed ultimately using TEM, are also described.
has issue date	2007-10-04(xsd:dateTime)
bibo:doi	10.1038/nprot.2007.304
bibo:pmid	17947985
has license	no-cc
sha1sum (hex)	c84ec5dc030fae18f4e9566b4739671073897b1f
schema:url	https://doi.org/10.1038/nprot.2007.304
resource representing a document's title	Processing tissue and cells for transmission electron microscopy in diagnostic pathology and research
has PubMed Central identifier	PMC7086545
has PubMed identifier	17947985
schema:publication	Nat Protoc
resource representing a document's body	covid:c84ec5dc030fae18f4e9566b4739671073897b1f#body_text
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