About: Construction of a non-infectious SARS coronavirus replicon for application in drug screening and analysis of viral protein function

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About: Construction of a non-infectious SARS coronavirus replicon for application in drug screening and analysis of viral protein function Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Construction of a non-infectious SARS coronavirus replicon for application in drug screening and analysis of viral protein function
Creator	Wang, Lin-Fa Shi, Zheng-Li Wang, Jian-Min
Source	Elsevier; Medline; PMC
abstract	Abstract Severe acute respiratory syndrome virus (SARS-CoV) was the causative agent of the SARS outbreaks in 2002–2003. A safer in vitro system is desirable for conducting research on SARS-CoV and to screen for antiviral drugs against the virus. Based on the infectious cDNA clone of rSARS-CoV-ΔE, in which the E gene has been deleted, a safe non-infectious replicon was constructed by replacing the S gene with the enhanced green fluorescent protein (eGFP) gene. Successful replication was achieved as evident from continuous expression of eGFP detected by both fluorescence and Western blot. Treatment with antiviral drugs demonstrated that the replication could be significantly inhibited by 0.4mg/ml of cysteine proteinase inhibitor E-64D, but not by ribavirin. The same replicons containing further deletion of the coding regions for non-structural proteins (nsp) 1, 2 or 16 confirmed previous observation that nsp16, but not nsp1 or nsp2, was essential for efficient viral replication or transcription.
has issue date	2008-09-12(xsd:dateTime)
bibo:doi	10.1016/j.bbrc.2008.06.129
bibo:pmid	18619943
has license	els-covid
sha1sum (hex)	c8d8115e39c0deb7314b1ac1cad4567ba287053d
schema:url	https://doi.org/10.1016/j.bbrc.2008.06.129
resource representing a document's title	Construction of a non-infectious SARS coronavirus replicon for application in drug screening and analysis of viral protein function
has PubMed Central identifier	PMC7092913
has PubMed identifier	18619943
schema:publication	Biochemical and Biophysical Research Communications
resource representing a document's body	covid:c8d8115e39c0deb7314b1ac1cad4567ba287053d#body_text
is schema:about of	named entity 'ribavirin' named entity 'inhibited' named entity 'replicon' named entity 'eGFP' named entity 'Western blot' named entity 'DRUG SCREENING' named entity 'NON-' named entity 'OBSERVATION' named entity 'NSP2' named entity 'WESTERN BLOT' named entity 'ANTIVIRAL DRUGS' named entity 'CONFIRMED' named entity 'REPLICATION' named entity 'EGFP' named entity 'CYSTEINE PROTEINASE INHIBITOR' named entity 'MG%' named entity 'INHIBITED' named entity 'CDNA CLONE' named entity 'CAUSATIVE AGENT' named entity 'CONDUCTING' named entity 'CONSTRUCTED' named entity 'PROTEIN FUNCTION' named entity 'REPLICON' named entity 'RIBAVIRIN' named entity 'SCREEN' named entity 'IN VITRO' named entity 'SAFE' named entity 'DETECTED' named entity 'E-64D' named entity 'ESSENTIAL' named entity 'EFFICIENT' named entity 'TREATMENT' named entity 'DEMONSTRATED' named entity 'BASED' named entity 'NSP1' named entity 'EXPRESSION' named entity 'VIRUS' named entity 'CONTINUOUS' named entity 'ENHANCED GREEN FLUORESCENT PROTEIN' named entity 'REPLACING' named entity 'CONTAINING' named entity 'CODING' named entity 'SARS-COV' named entity 'RESEARCH' named entity 'GENE' named entity 'OUTBREAKS' named entity 'TRANSCRIPTION' named entity 'VIRAL REPLICATION' named entity 'INFECTIOUS' named entity 'APPLICATION' named entity 'NON-' named entity 'SARS CORONAVIRUS' named entity 'VIRAL PROTEIN' named entity 'REPLICON' named entity 'INFECTIOUS' named entity 'PROTEIN ' named entity 'DELETED' named entity 'SUCCESSFUL' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'COULD BE' named entity 'SARS' named entity 'PREVIOUS' named entity 'NON-STRUCTURAL PROTEINS' named entity 'BUT' named entity 'DELETION' named entity 'REGIONS' named entity 'CONSTRUCTION' named entity 'SYSTEM' named entity 'FLUORESCENCE' named entity 'ANALYSIS' named entity '0.4' covid:arg/c8d8115e39c0deb7314b1ac1cad4567ba287053d named entity 'desirable' named entity 'green fluorescent protein' named entity 'replicons' named entity 'coding regions' named entity 'cysteine' named entity 'fluorescent protein' named entity 'cDNA'

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