About: Biological and morphological consequences of dsRNA-induced suppression of tetraspanin mRNA in developmental stages of Echinococcus granulosus

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About: Biological and morphological consequences of dsRNA-induced suppression of tetraspanin mRNA in developmental stages of Echinococcus granulosus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Biological and morphological consequences of dsRNA-induced suppression of tetraspanin mRNA in developmental stages of Echinococcus granulosus
Creator	Mousavi, Seyed Afgar, Ali Faridi, Ashkan Harandi, Majid Mohammadi, Mohammad Mortezaei, Seifollah Sadeghi, Balal
topic	covid:c967c20091c4331f0068ec5a3f5513a4e5ca29e9#this
Source	PMC
abstract	BACKGROUND: Cystic echinococcosis, caused by the cestode Echinococcus granulosus, is a neglected tropical disease with remarkable morbidity in humans and a problem of worldwide economic importance in livestock industry. Understanding the molecular basis of the parasite growth and development is essential for the disease diagnosis, management and control. The tetraspanin (TSP) family of proteins are transmembrane proteins with a role in many physiological processes of eukaryotic organisms. TSPs present in the tegumental surface of platyhelminths play pivotal roles in host-parasite interaction. However, little is known about the role of TSPs in growth and development in the Platyhelminthes. To understand the role of TSP1 in the growth and development of E. granulosus we investigated the effect of EgTSP1-specific long dsRNA in different in vitro stages of the parasite. METHODS: Different stages of E. granulosus, protoscoleces and strobilated worms, were cultivated In vitro in di-phasic media. Using long dsRNA and two delivery methods, i.e. electroporation and electro-soaking, EgTSP1 silencing was performed with an EgTSP1-specific dsRNA. The TSP1 expression profile was assessed as well as the biological and ultrastructural properties of the parasites. RESULTS: After three days of dsRNA treatment, EgTSP1 expression was significantly reduced in both stages of E. granulosus as compared to irrelevant/unrelated dsRNA and untreated controls. Silencing expression of EgTSP1 in different stages of E. granulosus resulted in reduced viability and body contractions, inhibition of protoscoleces evagination and distinctive tegumental changes. Ultrastructural morphology of the strobilated worms treated with EgTSP1-specific dsRNA was indicative of the microtriches impairments and vacuolated tegument compared to the control helminths. CONCLUSIONS: Results of the present study suggest that EgTSP1 plays important structural roles in tegument configuration in E. granulosus. EgTSP1 is proved to be a potential target for the development of vaccines and RNAi-based drugs. [Image: see text]
has issue date	2020-04-10(xsd:dateTime)
bibo:doi	10.1186/s13071-020-04052-y
bibo:pmid	32276648
has license	cc-by
sha1sum (hex)	c967c20091c4331f0068ec5a3f5513a4e5ca29e9
schema:url	https://doi.org/10.1186/s13071-020-04052-y
resource representing a document's title	Biological and morphological consequences of dsRNA-induced suppression of tetraspanin mRNA in developmental stages of Echinococcus granulosus
has PubMed Central identifier	PMC7146954
has PubMed identifier	32276648
schema:publication	Parasit Vectors
resource representing a document's body	covid:c967c20091c4331f0068ec5a3f5513a4e5ca29e9#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/c967c20091c4331f0068ec5a3f5513a4e5ca29e9
is schema:about of	named entity 'E. granulosus' named entity 'eukaryotic' named entity 'Understanding' named entity 'investigated' named entity 'Echinococcus' named entity 'PROTEINS' named entity 'TROPICAL DISEASE' named entity 'IN VITRO' named entity 'Cystic' named entity 'transmembrane proteins' named entity 'host-parasite interaction' named entity 'problem' named entity 'TSPs' named entity 'basis' named entity 'livestock' named entity 'molecular basis' named entity 'growth and development' named entity 'eukaryotic organisms' named entity 'TSP1' named entity 'tetraspanin' named entity 'E. granulosus' named entity 'growth and development' named entity 'dsRNA' named entity 'cestode' named entity 'Platyhelminthes' named entity 'dsRNA' named entity 'developmental stages' named entity 'parasite' named entity 'intermediate hosts' named entity 'parasites' named entity 'proteomics' named entity 'cox1' named entity 'GenBank' named entity 'biological consequences' named entity 'evagination' named entity 'tapeworms' named entity 'developmental stages' named entity 'dsRNA' named entity 'mRNA expression' named entity 'syncytial' named entity 'dsRNA' named entity 'developmental stages' named entity 'siRNA' named entity 'E. granulosus' named entity 'RNAi' named entity 'siRNA' named entity 'evagination' named entity 'dog' named entity 'vacuolated' named entity 'dsRNA' named entity 'SW3' named entity 'light microscope' named entity 'signal transduction pathways' named entity 'helminth' named entity 'S. mansoni' named entity 'S. mansoni' named entity 'eosin' named entity 'TSP-1' named entity 'essential elements' named entity 'RNase' named entity 'dsRNA' named entity 'parasite' named entity 'gene' named entity 'genome sequences' named entity 'RNAi' named entity 'sense and antisense' named entity 'E. granulosus' named entity 'cytoplasm' named entity 'evagination' named entity 'dsRNA' named entity 'motility' named entity 'ungulates' named entity 'phenotypic'

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