About: Identification of a Gamma Interferon-Activated Inhibitor of Translation-Like RNA Motif at the 3′ End of the Transmissible Gastroenteritis Coronavirus Genome Modulating Innate Immune Response

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Identification of a Gamma Interferon-Activated Inhibitor of Translation-Like RNA Motif at the 3′ End of the Transmissible Gastroenteritis Coronavirus Genome Modulating Innate Immune Response Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Identification of a Gamma Interferon-Activated Inhibitor of Translation-Like RNA Motif at the 3′ End of the Transmissible Gastroenteritis Coronavirus Genome Modulating Innate Immune Response
Creator	Enjuanes, Luis Nogales, Aitor Almazán, Fernando Marquez-Jurado, Silvia Zuñiga, Sonia
Source	Medline; PMC
abstract	A 32-nucleotide (nt) RNA motif located at the 3′ end of the transmissible gastroenteritis coronavirus (TGEV) genome was found to specifically interact with the host proteins glutamyl-prolyl-tRNA synthetase (EPRS) and arginyl-tRNA synthetase (RRS). This RNA motif has high homology in sequence and secondary structure with the gamma interferon-activated inhibitor of translation (GAIT) element, which is located at the 3′ end of several mRNAs encoding proinflammatory proteins. The GAIT element is involved in the translation silencing of these mRNAs through its interaction with the GAIT complex (EPRS, heterogeneous nuclear ribonucleoprotein Q, ribosomal protein L13a, and glyceraldehyde 3-phosphate dehydrogenase) to favor the resolution of inflammation. Interestingly, we showed that the viral RNA motif bound the GAIT complex and inhibited the in vitro translation of a chimeric mRNA containing this RNA motif. To our knowledge, this is the first GAIT-like motif described in a positive RNA virus. To test the functional role of the GAIT-like RNA motif during TGEV infection, a recombinant coronavirus harboring mutations in this motif was engineered and characterized. Mutations of the GAIT-like RNA motif did not affect virus growth in cell cultures. However, an exacerbated innate immune response, mediated by the melanoma differentiation-associated gene 5 (MDA5) pathway, was observed in cells infected with the mutant virus compared with the response observed in cells infected with the parental virus. Furthermore, the mutant virus was more sensitive to beta interferon than the parental virus. All together, these data strongly suggested that the viral GAIT-like RNA motif modulates the host innate immune response.
has issue date	2015-03-10(xsd:dateTime)
bibo:doi	10.1128/mbio.00105-15
bibo:pmid	25759500
has license	cc-by-nc-sa
sha1sum (hex)	c99640294d5862d4482de51f9a442deaf09b53be
schema:url	https://doi.org/10.1128/mbio.00105-15
resource representing a document's title	Identification of a Gamma Interferon-Activated Inhibitor of Translation-Like RNA Motif at the 3′ End of the Transmissible Gastroenteritis Coronavirus Genome Modulating Innate Immune Response
has PubMed Central identifier	PMC4453530
has PubMed identifier	25759500
schema:publication	mBio
resource representing a document's body	covid:c99640294d5862d4482de51f9a442deaf09b53be#body_text
is schema:about of	named entity 'GENOME' named entity 'DISRUPTION' named entity 'CORONAVIRUS' named entity 'GLUTAMYL-PROLYL-TRNA SYNTHETASE' named entity 'HARBORING' named entity 'RELEVANCE' named entity 'GAMMA INTERFERON' named entity 'CELL CULTURES' named entity 'TRIGGERED BY' named entity 'ITS' named entity 'POSITIVE' named entity 'INFLAMMATION' named entity 'TO TEST' named entity 'RESPONSE' named entity 'LED' named entity 'ENCODE' named entity 'SYNTHESIS' named entity 'SILENCING' named entity 'INDICATING' named entity 'CHARACTERIZED' named entity 'BOUND' named entity 'ANTIVIRAL RESPONSE' named entity 'SENSITIVE TO' named entity 'MELANOMA DIFFERENTIATION-ASSOCIATED GENE 5' named entity 'RIBOSOMAL PROTEIN L13A' named entity 'CONTAINING' named entity 'EXACERBATED' named entity 'PROINFLAMMATORY CYTOKINES' named entity 'LINE' named entity 'BUT' named entity 'BASIS' named entity 'ACTIVATED' named entity 'DESCRIBED' named entity 'VIRUS REPLICATION' named entity 'innate immune response' named entity 'encode' named entity 'inhibitor' named entity 'data' named entity 'virus' named entity 'infected' named entity 'parental' named entity 'coronaviruses' named entity 'viral' named entity 'RNA' named entity 'Response' named entity 'Inhibitor' named entity 'LIKE' named entity 'INHIBITOR' named entity 'INTERACT' named entity 'MODULATION' named entity 'MAY BE' named entity 'HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN Q' named entity 'RECOMBINANT' named entity 'ENCODING' named entity 'INTERFERON ' named entity 'SECONDARY STRUCTURE' named entity 'MUTANT' named entity 'DIFFERENT' named entity 'STRATEGIES' named entity 'LEVELS' named entity 'INHIBITED' named entity 'FINDINGS' named entity 'INFECTED' named entity 'ELEMENT' named entity 'ARGINYL-TRNA SYNTHETASE' named entity 'DEVELOPMENT' named entity 'IN VITRO TRANSLATION' named entity 'FUNCTIONAL ROLE' named entity 'CELLS' named entity 'GAMMA INTERFERON' named entity 'END' named entity 'ACTIVATED' named entity 'TRANSLATION' named entity 'KNOWLEDGE' named entity 'PARENTAL' named entity 'NUCLEOTIDE' named entity 'NOVEL'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software