About: Discovery and Targeted LC-MS/MS of Purified Polerovirus Reveals Differences in the Virus-Host Interactome Associated with Altered Aphid Transmission

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About: Discovery and Targeted LC-MS/MS of Purified Polerovirus Reveals Differences in the Virus-Host Interactome Associated with Altered Aphid Transmission Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Discovery and Targeted LC-MS/MS of Purified Polerovirus Reveals Differences in the Virus-Host Interactome Associated with Altered Aphid Transmission
Creator	Bereman, Michael Cilia, Michelle Fish, Tara Gildow, Fredrick Gray, Stewart Howe, Kevin Peter, Kari Smith, Dawn Thannhauser, Theodore Maccoss, Michael
Source	PMC
abstract	Circulative transmission of viruses in the Luteoviridae, such as cereal yellow dwarf virus (CYDV), requires a series of precisely orchestrated interactions between virus, plant, and aphid proteins. Natural selection has favored these viruses to be retained in the phloem to facilitate acquisition and transmission by aphids. We show that treatment of infected oat tissue homogenate with sodium sulfite reduces transmission of the purified virus by aphids. Transmission electron microscopy data indicated no gross change in virion morphology due to treatments. However, treated virions were not acquired by aphids through the hindgut epithelial cells and were not transmitted when injected directly into the hemocoel. Analysis of virus preparations using nanoflow liquid chromatography coupled to tandem mass spectrometry revealed a number of host plant proteins co-purifying with viruses, some of which were lost following sodium sulfite treatment. Using targeted mass spectrometry, we show data suggesting that several of the virus-associated host plant proteins accumulated to higher levels in aphids that were fed on CYDV-infected plants compared to healthy plants. We propose two hypotheses to explain these observations, and these are not mutually exclusive: (a) that sodium sulfite treatment disrupts critical virion-host protein interactions required for aphid transmission, or (b) that host infection with CYDV modulates phloem protein expression in a way that is favorable for virus uptake by aphids. Importantly, the genes coding for the plant proteins associated with virus may be examined as targets in breeding cereal crops for new modes of virus resistance that disrupt phloem-virus or aphid-virus interactions.
has issue date	2012-10-30(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0048177
bibo:pmid	23118947
has license	cc0
sha1sum (hex)	c9fca01d18bac485ae1c5257a712c516aeea8408
schema:url	https://doi.org/10.1371/journal.pone.0048177
resource representing a document's title	Discovery and Targeted LC-MS/MS of Purified Polerovirus Reveals Differences in the Virus-Host Interactome Associated with Altered Aphid Transmission
has PubMed Central identifier	PMC3484124
has PubMed identifier	23118947
schema:publication	PLoS One
resource representing a document's body	covid:c9fca01d18bac485ae1c5257a712c516aeea8408#body_text
is schema:about of	named entity 'aphids' named entity 'coding' named entity 'interactions' named entity 'preparations' named entity 'sodium sulfite' named entity 'disrupt' named entity 'orchestrated' named entity 'plants' named entity 'acquired' named entity 'transmitted' named entity 'aphid' named entity 'Interactome' covid:arg/c9fca01d18bac485ae1c5257a712c516aeea8408 named entity 'Luteoviridae' named entity 'series' named entity 'Natural selection' named entity 'virus' named entity 'liquid chromatography' named entity 'data' named entity 'phloem' named entity 'interactions' named entity 'homogenate' named entity 'tissue' named entity 'mass spectrometry' named entity 'proteins' named entity 'transmission' named entity 'protein expression' named entity 'hemocoel' named entity 'aphids' named entity 'liquid chromatography' named entity 'virus' named entity 'aphid' named entity 'aphids' named entity 'virus' named entity 'Transmission electron microscopy' named entity 'virion' named entity 'epithelial cells' named entity 'virus' named entity 'virus' named entity 'tandem mass spectrometry' named entity 'infection' named entity 'yellow dwarf' named entity 'Polerovirus' named entity 'change' named entity 'transmissibility' named entity 'amino acids' named entity 'aphid' named entity 'single stranded RNA' named entity 'mass spectrometry' named entity 'phloem' named entity 'peptide' named entity 'peptides' named entity 'disulfide bonding' named entity 'proteomes' named entity 'phloem' named entity 'plant viruses' named entity 'ion' named entity 'virions' named entity 'Microinjector' named entity 'aphid' named entity 'divalent' named entity 'RTP' named entity 'natural selection' named entity 'oat seedlings' named entity 'aphid' named entity 'random process' named entity 'thaumatin' named entity 'virus transmission' named entity 'virions' named entity 'ribosomal proteins' named entity 'EDTA' named entity 'Torrance, CA'

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