About: Attenuation of Mouse Hepatitis Virus by Deletion of the LLRKxGxKG Region of Nsp1

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Attenuation of Mouse Hepatitis Virus by Deletion of the LLRKxGxKG Region of Nsp1
Creator	Xiang, H Baojun, L Lei, L Yi, Y Ying, S
Source	Medline; PMC
abstract	Coronaviruses are a family of large positive-sense RNA viruses that are responsible for a wide range of important veterinary and human diseases. Nsp1 has been shown to have an important role in the pathogenetic mechanisms of coronaviruses in vivo. To assess the function of a relatively conserved domain (LLRKxGxKG) of MHV nsp1, a mutant virus, MHV-nsp1-27D, with a 27 nts (LLRKxGxKG) deletion in nsp1, was constructed using a reverse genetic system with a vaccinia virus vector. The mutant virus had similar growth kinetics to MHV-A59 wild-type virus in 17CI-1 cells, but was highly attenuated in vivo. Moreover, the mutant virus completely protected C57BL/6 mice from a lethal MHV-A59 challenge. To further analyze the mechanism of the attenuation of the mutant virus, changes in reporter gene expression were measured in nsp1- or nsp1-27D-expressing cells; the results showed that nsp1 inhibited reporter gene expression controlled by different promoters, but that this inhibition was reduced for nsp1-27D. The research in vivo and in vitro suggests that the LLRKxGxKG region of nsp1 may play an important role in this process.
has issue date	2013-04-08(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0061166
bibo:pmid	23593419
has license	cc-by
sha1sum (hex)	ca0cc9c9da1307a7f36276eea4d117d6f74c2d7f
schema:url	https://doi.org/10.1371/journal.pone.0061166
resource representing a document's title	Attenuation of Mouse Hepatitis Virus by Deletion of the LLRKxGxKG Region of Nsp1
has PubMed Central identifier	PMC3620170
has PubMed identifier	23593419
schema:publication	PLoS One
resource representing a document's body	covid:ca0cc9c9da1307a7f36276eea4d117d6f74c2d7f#body_text
is schema:about of	covid:arg/ca0cc9c9da1307a7f36276eea4d117d6f74c2d7f named entity 'pathogenetic' named entity 'MHV' named entity 'Mouse Hepatitis Virus' named entity 'China' named entity 'cysteine' named entity 'hepatotropic' named entity 'MHV' named entity 'methyltransferase' named entity 'mice' named entity 'MHV' named entity 'MHV' named entity 'MHV' named entity 'plasmids' named entity 'MHV' named entity 'Recombinant' named entity 'reporter gene' named entity 'hepatitis' named entity 'cloned' named entity 'MHV' named entity 'HeLa' named entity 'gene' named entity 'viruses' named entity 'Escherichia coli' named entity 'MHV' named entity 'live vaccine' named entity 'C-terminal' named entity 'MHV' named entity '0.01' named entity 'chloramphenicol acetyltransferase' named entity 'live attenuated' named entity 'nucleotides' named entity 'protein' named entity 'antiviral' named entity 'proteinases' named entity 'PBS' named entity 'polyprotein' named entity 'liver' named entity 'plasmid' named entity 'plasmids' named entity '0.01' named entity 'viruses' named entity 'mice' named entity 'Mouse Hepatitis Virus' named entity 'transfected' named entity 'replicative capacity' named entity 'mouse hepatitis virus' named entity 'protein' named entity 'acute hepatitis' named entity 'Roche' named entity 'reporter gene assay' named entity 'MHV' named entity 'viruses' named entity 'MHV' named entity 'adsorption' named entity 'virus' named entity 'CoV' named entity 'spleen' named entity 'luciferase' named entity 'C-terminal' named entity 'plasmids' named entity 'paired Student's t-test' named entity 'myc' named entity 'MHV' named entity 'virus' named entity 'reverse genetic' named entity 'Immunization' named entity 'MHV' named entity 'spleen' named entity 'MHV' named entity 'virus replication' named entity 'MHV' named entity 'China' named entity 'reporter gene' named entity 'serum' named entity 'MHV' named entity 'gene'

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