About: An In Vitro Microneutralization Assay for SARS‐CoV‐2 Serology and Drug Screening

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	An In Vitro Microneutralization Assay for SARS‐CoV‐2 Serology and Drug Screening
Creator	Miorin, Lisa García-Sastre, Adolfo Krammer, Florian Martinez-Sobrido, Luis Simon, Viviana Liu, Wen-Chun Moran, Thomas Albrecht, Randy White, Kris Amanat, Fatima Mcmahon, Meagan Meade, Philip Strohmeier, Shirin
source	Medline; PMC
abstract	The severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) emerged in the city of Wuhan, Hubei Province, China, in late 2019. Since then, the virus has spread globally and caused a pandemic. Assays that can measure the antiviral activity of antibodies or antiviral compounds are needed for SARS‐CoV‐2 vaccine and drug development. Here, we describe in detail a microneutralization assay, which can be used to assess in a quantitative manner if antibodies or drugs can block entry and/or replication of SARS‐CoV‐2 in vitro. © 2020 Wiley Periodicals LLC. Basic Protocol 1: Microneutralization assay to test inhibition of virus by antibodies (purified antibodies or serum/plasma) Basic Protocol 2: Screening of anti‐SARS‐CoV‐2 compounds in vitro Support Protocol: SARS‐CoV‐2 propagation
has issue date	2020-06-25(xsd:dateTime)
bibo:doi	10.1002/cpmc.108
bibo:pmid	32585083
has license	no-cc
sha1sum (hex)	caa91a7e09a2d3b7cf7bd719e86deb186f748ccd
schema:url	https://doi.org/10.1002/cpmc.108
resource representing a document's title	An In Vitro Microneutralization Assay for SARS‐CoV‐2 Serology and Drug Screening
has PubMed Central identifier	PMC7361222
has PubMed identifier	32585083
schema:publication	Curr Protoc Microbiol
resource representing a document's body	covid:caa91a7e09a2d3b7cf7bd719e86deb186f748ccd#body_text
is schema:about of	named entity 'SARS-CoV-2' named entity 'antiviral activity' named entity 'coronavirus' named entity 'infection' named entity 'MTT assay' named entity 'cytotoxic' named entity 'SARS-CoV-2' named entity 'growth medium' named entity 'cell line' named entity 'China' named entity 'BSL-2' named entity 'severe acute respiratory syndrome coronavirus 2' named entity 'antibodies' named entity 'MTT assay' named entity 'SARS-CoV-2' named entity 'antibody' named entity 'coronaviruses' named entity 'DMSO' named entity 'infection' named entity 'data analysis' named entity 'supernatants' named entity 'human population' named entity 'Positive and negative controls' named entity 'virus' named entity 'biosafety cabinet' named entity 'infection' named entity 'BSL-3' named entity 'Microplate Reader' named entity 'Non-linear regression' named entity 'virus' named entity 'DAPI' named entity 'MTT assay' named entity 'reagent' named entity 'cell lines' named entity 'cytotoxicity' named entity '96-well plate' named entity 'antiviral activity' named entity 'cell culture' named entity 'cell death' named entity 'neutralizing antibodies' named entity 'nucleoprotein' named entity 'virus' named entity 'positive and negative controls' named entity 'antivirals' named entity 'cell culture' named entity 'infection' named entity 'virus' named entity '37°C' named entity 'SARS-CoV2' named entity 'cytotoxic' named entity 'antibody' named entity 'microneutralization' named entity 'DMEM' named entity 'plaque assay' named entity 'toxicity' named entity 'Microbiology' named entity '96-well plate' named entity 'cell lines' named entity 'Krammer' named entity 'immunostaining' named entity 'viral replication' named entity 'cell line' named entity '2-phenylindole' named entity 'cytometer' named entity 'formaldehyde' named entity 'antiviral' named entity 'immunostain' named entity 'Incubate' named entity 'Incubate'

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