About: Determining the impact of commercial feed additives as potential porcine epidemic diarrhea virus mitigation strategies as determined by polymerase chain reaction analysis and bioassay()

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About: Determining the impact of commercial feed additives as potential porcine epidemic diarrhea virus mitigation strategies as determined by polymerase chain reaction analysis and bioassay() Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Determining the impact of commercial feed additives as potential porcine epidemic diarrhea virus mitigation strategies as determined by polymerase chain reaction analysis and bioassay()
Creator	Chen, Qi Zhang, Jianqiang Main, Rodger Bai, Jianfa Jones, Cassandra Dritz, Steve Stark, Charles Woodworth, Jason Bergstrom, Jon Derouchey, Joel Gauger, Philip Gebhardt, Jordan Goodband, Robert Tokach, Mike
topic	covid:cac5cab0acc600d9346ead6e63b17f06e681fe6d#this
Source	Medline; PMC
abstract	Mitigation of porcine epidemic diarrhea virus (PEDV) was assessed using two feed additives (0.5% inclusion of a benzoic acid [BA] product and 0.02% inclusion of an essential oil [EO] product; DSM Nutritional Products Inc., Parsippany, NJ), and combination of both products (0.5% BA and 0.02% EO) in spray-dried porcine plasma (SDPP) and a swine gestation diet (FEED) as determined by real-time quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and bioassay. Viral RNA quantification was performed at 7 sampling days post-laboratory inoculation (d 0, 1, 3, 7, 14, 21, and 42) and infectivity was assessed via bioassay with 10-d-old pigs. There was a tendency for treatment × feed matrix × day interaction (P = 0.094), in which the cycle threshold (Ct) value increased over time in FEED when treated with both feed additives, whereas there was no increase over time observed in SDPP treated with both feed additives. There was a feed matrix × day interaction (P < 0.001) in which Ct increased over time in FEED, whereas very little increase over time was observed in SDPP. A tendency for a treatment × feed matrix effect (P = 0.085) was observed where FEED treated with the combination of EO and BA had a greater (P < 0.05) PEDV Ct value than other FEED treatments, and all SDPP treatments had the lower PEDV Ct values compared to FEED treatments (P < 0.05). Overall, the combination of both feed additives was most effective at reducing the quantity of genetic material as detected by qRT-PCR (P < 0.001) compared to either additive alone or no feed additive. Virus shedding was observed in the d 7 postinoculation SDPP treatment that was treated with both feed additives, as well as d 0 untreated FEED and d 0 FEED treated with both feed additives. No other treatment bioassay room had detectible RNA shed and detected in fecal swabs or cecal contents. In summary, the combination of EO and BA enhanced the degradation of PEDV RNA in feed but had little impact on RNA degradation in SDPP. Both untreated feed and feed treated with the combination of EO and BA resulted in infection at d 0 post-laboratory inoculation; however, neither set of samples was infective at d 1 postinoculation. In addition, SDPP harbored greater levels of quantifiable RNA for a longer duration of time compared to FEED, and these viral particles remained viable for a longer duration of time indicating differences in viral stability exist between different feed matrices.
has issue date	2018-08-20(xsd:dateTime)
bibo:doi	10.1093/tas/txy100
bibo:pmid	32289110
has license	cc-by-nc
sha1sum (hex)	cac5cab0acc600d9346ead6e63b17f06e681fe6d
schema:url	https://doi.org/10.1093/tas/txy100
resource representing a document's title	Determining the impact of commercial feed additives as potential porcine epidemic diarrhea virus mitigation strategies as determined by polymerase chain reaction analysis and bioassay()
has PubMed Central identifier	PMC7107256
has PubMed identifier	32289110
schema:publication	Transl Anim Sci
resource representing a document's body	covid:cac5cab0acc600d9346ead6e63b17f06e681fe6d#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/cac5cab0acc600d9346ead6e63b17f06e681fe6d
is schema:about of	named entity 'DETERMINED BY' named entity 'STRATEGIES' named entity 'VIRAL PARTICLES' named entity 'INFECTIVITY' named entity 'INTERACTION' named entity 'MITIGATION' named entity 'GENETIC MATERIAL' named entity 'CECAL' named entity 'GREATER' named entity 'PRODUCTS' named entity 'STABILITY' named entity 'PCR' named entity 'EFFECTIVE' named entity 'DETECTED' named entity 'BENZOIC ACID' named entity 'INC' named entity 'SET' named entity 'CONTENTS' named entity 'PRODUCT' named entity 'BIOASSAY' named entity 'FEED ADDITIVE' named entity 'COMBINATION' named entity 'DIET' named entity 'QUANTITY' named entity 'RNA DEGRADATION' named entity 'CT VALUE' named entity 'DSM' named entity 'REDUCING' named entity 'ESSENTIAL OIL' named entity 'SUMMARY' named entity 'INCREASE' named entity 'ASSESSED' named entity 'LITTLE' named entity 'ENHANCED' named entity 'Mitigation' named entity 'viral' named entity 'qRT-PCR' named entity 'compared' named entity 'viral' named entity 'quantifiable' named entity 'cecal' named entity 'cycle' named entity 'There' named entity 'INDICATING' named entity 'QUANTITATIVE REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION' named entity 'ADDITION' named entity 'VIABLE' named entity 'FEED' named entity 'TREATMENT' named entity 'MATRIX' named entity 'VALUES' named entity 'HARBORED' named entity 'PORCINE' named entity '0.001' named entity 'DIFFERENT' named entity 'CYCLE THRESHOLD' named entity 'NUTRITIONAL PRODUCTS' named entity 'QRT-PCR' named entity 'OLD' named entity 'OBSERVED' named entity 'BUT' named entity 'LEVELS' named entity 'REAL-TIME' named entity 'USING' named entity 'ADDITIVES' named entity 'MITIGATION'

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