About: Development of universal SYBR Green real-time RT-PCR for the rapid detection and quantitation of bovine and porcine toroviruses

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About: Development of universal SYBR Green real-time RT-PCR for the rapid detection and quantitation of bovine and porcine toroviruses Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of universal SYBR Green real-time RT-PCR for the rapid detection and quantitation of bovine and porcine toroviruses
Creator	Cho, Kyoung-Oh Kang, Mun-Il Kim, Ha-Hyun Park, Sang-Ik Park, Su-Jin Jeong, Young-Ju Kim, Hyun-Jeong Kwon, Hyung-Jun Park, Jun-Gyu Hosmillo, Myra Alfajaro, Mia Collantes, Therese
Source	Elsevier; Medline; PMC
abstract	Toroviruses (ToVs) are a group of emerging viruses that cause gastroenteritis in domestic animals and humans. Currently, methods such as real-time reverse transcription-polymerase chain reaction (real-time RT-PCR) have not yet been developed for the rapid detection and quantitation of bovine (BToV) and porcine (PToV) toroviruses. Using BToV and PToV RNA standards generated by in vitro transcription, the detection limit of the SYBR Green real-time RT-PCR assay was 2.54 × 10(2) BToV and 2.17 × 10(3) PToV copies/reaction (correlation coefficiency = 0.99 and 0.97, respectively), whereas those of RT-PCR and nested PCR were 2.54 × 10(5) and 2.54 × 10(4) (BToV) and 2.17 × 10(7) and 2.17 × 10(5) (PToV) cRNA viral copies/reaction, respectively. Archived diarrhea specimens of calves (n = 121) and piglets (n = 86) were subjected to RT-PCR, nested PCR and SYBR Green real-time RT-PCR. By conventional RT-PCR, 1 (0.8%) bovine and 7 (8.1%) porcine samples tested positive to BToV and PToV, respectively. With nested PCR, 13 (10.7%) bovine and 17 (19.8%) porcine samples tested positive. SYBR Green real-time RT-PCR assay detected BToV and PToV in 22 of 121 (18.2%) bovine and 31 of 86 (36.0%) porcine samples. These results indicate that SYBR Green real-time RT-PCR (P < 0.05) is a more sensitive assay, which can be reproduced as a reliable, sensitive, and rapid tool for the detection and quantitation of toroviruses.
has issue date	2010-06-15(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2010.06.001
bibo:pmid	20558206
has license	no-cc
sha1sum (hex)	cacae08c1b352c6a4aeba0e941454655a9e118cb
schema:url	https://doi.org/10.1016/j.jviromet.2010.06.001
resource representing a document's title	Development of universal SYBR Green real-time RT-PCR for the rapid detection and quantitation of bovine and porcine toroviruses
has PubMed Central identifier	PMC7112831
has PubMed identifier	20558206
schema:publication	J Virol Methods
resource representing a document's body	covid:cacae08c1b352c6a4aeba0e941454655a9e118cb#body_text
is schema:about of	named entity 'real-time' named entity 'SYBR Green' named entity 'humans' named entity 'rapid' named entity 'realtime' named entity 'assay' named entity 'real-time' named entity 'nested PCR' named entity 'porcine' named entity 'domestic animals' named entity 'detection' named entity 'PORCINE' named entity 'REAL-TIME RT-PCR' named entity '28P' named entity 'USING' named entity 'PORCINE' named entity 'REVERSE TRANSCRIPTION' named entity 'DETECTED' named entity 'ASSAY' named entity 'DETECTION' named entity 'A GROUP' named entity 'CAUSE' named entity 'DOMESTIC ANIMALS' named entity 'SPECIMENS' named entity 'GASTROENTERITIS' named entity 'CRNA' named entity 'REAL-TIME' named entity 'POSITIVE' named entity '0.99' named entity 'CURRENTLY' named entity 'TESTED' named entity 'TOROVIRUSES' named entity 'RT-PCR ASSAY' named entity 'SYBR GREEN' named entity 'GREEN ' named entity 'REAL-TIME RT-PCR' named entity 'COPIES' named entity 'GREEN ' named entity 'SENSITIVE' named entity 'CONVENTIONAL' named entity 'RAPID' named entity 'QUANTITATION' named entity 'BOVINE' named entity 'SYBR GREEN' named entity 'THESE' named entity 'ARCHIVED' named entity 'GENERATED' named entity 'DIARRHEA' named entity 'HAVE' named entity 'UNIVERSAL' named entity 'RNA' named entity 'RT-PCR' named entity 'IS A' named entity 'STANDARDS' named entity '0.05' named entity 'NESTED PCR ' named entity 'POLYMERASE CHAIN REACTION' named entity 'NESTED PCR' named entity 'CALVES' named entity 'TOOL' named entity 'BOVINE' named entity 'IN VITRO TRANSCRIPTION' named entity 'RELIABLE' named entity '288' named entity 'TOROVIRUSES' named entity 'VIRAL' named entity 'DETECTION LIMIT' named entity 'RESULTS' named entity '2818' named entity 'DEVELOPMENT'

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