About: Rolling circle amplification as isothermal gene amplification in molecular diagnostics

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About: Rolling circle amplification as isothermal gene amplification in molecular diagnostics Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Rolling circle amplification as isothermal gene amplification in molecular diagnostics
Creator	Kim, Dong-Eun Goo, Nam-In
source	Medline; PMC
abstract	Rolling circle amplification (RCA) developed in the mid-1990s has been widely used as an efficient isothermal DNA amplification process for molecular diagnosis. This enzymatic process amplifies target DNA sequences with high fidelity and specificity by using the strand displacing DNA polymerases. The product of RCA is long single-stranded DNA that contains tandem repeat of target sequence. Isothermal reaction amplification condition of RCA has an advantage over conventional polymerase chain reaction, because no temperature cycling devices are needed for RCA. Thus, RCA is suitable tool for point-of-care detection of target nucleic acids as well as facile detection of target genes. Combined with various detection methods, RCA could amplify and detect femtomolar scale of target nucleic acids with a specificity of one or two base discrimination. Herein, RCA technology is reviewed with an emphasis on molecular diagnosis of microRNAs, infectious pathogens, and point mutations.
has issue date	2016-07-29(xsd:dateTime)
bibo:doi	10.1007/s13206-016-0402-6
bibo:pmid	32226587
has license	no-cc
sha1sum (hex)	cb2ac105735bcf360963ae001284f4dd3ed8eff9
schema:url	https://doi.org/10.1007/s13206-016-0402-6
resource representing a document's title	Rolling circle amplification as isothermal gene amplification in molecular diagnostics
has PubMed Central identifier	PMC7096790
has PubMed identifier	32226587
schema:publication	Biochip J
resource representing a document's body	covid:cb2ac105735bcf360963ae001284f4dd3ed8eff9#body_text
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