About: The Infectious Bronchitis Coronavirus Envelope Protein Alters Golgi pH To Protect the Spike Protein and Promote the Release of Infectious Virus

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: The Infectious Bronchitis Coronavirus Envelope Protein Alters Golgi pH To Protect the Spike Protein and Promote the Release of Infectious Virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The Infectious Bronchitis Coronavirus Envelope Protein Alters Golgi pH To Protect the Spike Protein and Promote the Release of Infectious Virus
Creator	Machamer, Carolyn Westerbeck, Jason
Source	Medline; PMC
abstract	Coronaviruses (CoVs) assemble by budding into the lumen of the early Golgi complex prior to exocytosis. The small CoV envelope (E) protein plays roles in assembly, virion release, and pathogenesis. CoV E has a single hydrophobic domain (HD), is targeted to Golgi membranes, and has cation channel activity in vitro. The E protein from avian infectious bronchitis virus (IBV) has dramatic effects on the secretory system, which require residues in the HD. Mutation of the HD of IBV E in a recombinant virus background results in impaired growth kinetics, impaired release of infectious virions, accumulation of IBV spike (S) protein on the plasma membrane compared to wild-type (WT) IBV-infected cells, and aberrant cleavage of IBV S on virions. We previously reported the formation of two distinct oligomeric pools of IBV E in transfected and infected cells. Disruption of the secretory pathway by IBV E correlates with a form that is likely monomeric, suggesting that the effects on the secretory pathway are independent of E ion channel activity. Here, we present evidence suggesting that the monomeric form of IBV E correlates with an increased Golgi luminal pH. Infection with IBV or expression of IBV E induces neutralization of Golgi pH, promoting a model in which IBV E alters the secretory pathway through interaction with host cell factors, protecting IBV S from premature cleavage and leading to the efficient release of infectious virus from the cells. This is the first demonstration of a coronavirus-induced alteration in the microenvironment of the secretory pathway. IMPORTANCE Coronaviruses are important human pathogens with significant zoonotic potential. Progress has been made toward identifying potential vaccine candidates for highly pathogenic human CoVs, including the use of attenuated viruses that lack the CoV E protein or express E mutants. However, no approved vaccines or antiviral therapeutics exist. Understanding the role of the CoV E protein in virus assembly and release is thus an important prerequisite for potential vaccines as well as in identifying novel antiviral therapeutics.
has issue date	2019-03-13(xsd:dateTime)
bibo:doi	10.1128/jvi.00015-19
bibo:pmid	30867314
has license	bronze-oa
sha1sum (hex)	cbb9ec2328fdea46172cc0dd7f8fa8be82256c93
schema:url	https://doi.org/10.1128/jvi.00015-19
resource representing a document's title	The Infectious Bronchitis Coronavirus Envelope Protein Alters Golgi pH To Protect the Spike Protein and Promote the Release of Infectious Virus
has PubMed Central identifier	PMC6532078
has PubMed identifier	30867314
schema:publication	Journal of Virology
resource representing a document's body	covid:cbb9ec2328fdea46172cc0dd7f8fa8be82256c93#body_text
is schema:about of	named entity 'plasma membrane' named entity 'microenvironment' named entity 'IBV' named entity 'cation' named entity 'transfected' named entity 'luminal' named entity 'pathogens' named entity 'factors' named entity 'potential' named entity 'residues' named entity 'host' named entity 'effects' named entity 'Disruption' named entity 'membranes' named entity 'PROTECTING' named entity 'PREVIOUSLY' named entity 'MUTATION' named entity 'ZOONOTIC' named entity 'EXIST' named entity 'ANTIVIRAL' named entity 'CATION CHANNEL ACTIVITY' named entity 'LIKELY' named entity 'RESULTS' named entity 'UNDERSTANDING' named entity 'HERE' named entity 'ALTERATION' named entity 'PROMOTING' named entity 'THERAPEUTICS' named entity 'SIGNIFICANT' named entity 'COMPARED' named entity 'HUMAN' named entity 'GROWTH KINETICS' named entity 'NEUTRALIZATION' named entity 'IDENTIFYING' named entity 'VIRIONS' named entity 'INDEPENDENT OF' named entity 'PROTEIN ' named entity 'FORMATION' named entity 'identifying' named entity 'virion' named entity 'However' named entity 'interaction' named entity 'early' named entity 'Golgi' named entity 'infectious bronchitis' named entity 'IBV' named entity 'mutants' named entity 'vaccine' named entity 'Golgi' named entity 'This' named entity 'IBV' named entity 'cells' named entity 'prior' named entity 'exocytosis' named entity 'IBV' named entity 'channel' named entity 'correlates' named entity 'human' named entity 'Coronaviruses' named entity 'IBV' named entity 'ion channel' named entity 'protein' named entity 'virions' named entity 'zoonotic potential' named entity 'vaccines' named entity 'virion' named entity 'IBV' named entity 'IBV' named entity 'cation channel' named entity 'secretory pathway' named entity 'Infectious Bronchitis' named entity 'HeLa' named entity 'luminal' named entity 'IBV' named entity 'secretory pathway' named entity 'Golgi' named entity 'IBV' named entity 'protein' named entity 'IAV' named entity '14,000'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software