About: Nanotrap® particles improve detection of SARS-CoV-2 for pooled sample methods, extraction-free saliva methods, and extraction-free transport medium methods

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About: Nanotrap® particles improve detection of SARS-CoV-2 for pooled sample methods, extraction-free saliva methods, and extraction-free transport medium methods Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Nanotrap® particles improve detection of SARS-CoV-2 for pooled sample methods, extraction-free saliva methods, and extraction-free transport medium methods
Creator	Miao, ; Kelly, ; Akhrymuk, ; Andersen, ; Barbero, ; Barksdale, ; Callahan, ; Dunlap, ; Goldfarb, ; Jones-Roe, ; Kehn-Hall, ; Kim B, ; Lehman, ; Lepene, ; Munns, ; Patel, ; Patnaik, ; Porter, ; Ra, Barclay Ramsey, ; Sahoo, ; Swahn, ; Warsh, ;
source	BioRxiv
abstract	Here we present a rapid and versatile method for capturing and concentrating SARS-CoV-2 from transport medium and saliva using affinity-capture magnetic hydrogel particles. We demonstrate that the method concentrates virus prior to RNA extraction, thus significantly improving detection of the virus using a real-time RT-PCR assay across a range of viral titers, from 100 to 1,000,000 viral copies/mL; in particular, detection of virus in low viral load samples is enhanced when using the method coupled with the IDT 2019-nCoV CDC EUA Kit. This method is compatible with commercially available nucleic acid extraction kits, as well with a simple heat and detergent method. Using transport medium diagnostic remnant samples that previously had been tested for SARS-CoV-2 using either the Abbott RealTime SARS-CoV-2 EUA Test (n=14) or the Cepheid Xpert Xpress SARS-CoV-2 EUA Test (n=35), we demonstrate that our method not only correctly identifies all positive samples (n = 17) but also significantly improves detection of the virus in low viral load samples. The average improvement in cycle threshold (Ct) value as measured with the IDT 2019-nCoV CDC EUA Kit was 3.1; n = 10. Finally, to demonstrate that the method could potentially be used to enable pooled testing, we spiked infectious virus or a confirmed positive diagnostic remnant sample into 5 mL and 10 mL of negative transport medium and observed significant improvement in the detection of the virus from those larger sample volumes.
has issue date	2020-06-29(xsd:dateTime)
bibo:doi	10.1101/2020.06.25.172510
has license	biorxiv
sha1sum (hex)	cc0408829d021cfa07da7d43ae6e2b47dc01bba9
schema:url	https://doi.org/10.1101/2020.06.25.172510
resource representing a document's title	Nanotrap® particles improve detection of SARS-CoV-2 for pooled sample methods, extraction-free saliva methods, and extraction-free transport medium methods
schema:publication	bioRxiv
resource representing a document's body	covid:cc0408829d021cfa07da7d43ae6e2b47dc01bba9#body_text
is schema:about of	named entity 'sample' named entity 'enable' named entity 'commercially' named entity 'titers' named entity 'range' named entity 'virus' named entity 'transport medium' named entity 'IDT' named entity 'copies' named entity 'POOLED' named entity '3.1' named entity 'DETECTION OF VIRUS' named entity 'TEST' named entity 'COMPATIBLE WITH' named entity 'OBSERVED' named entity 'REMNANT' named entity 'SAMPLES' named entity 'CONCENTRATING' named entity 'AVERAGE' named entity 'detergent' named entity 'detection' named entity 'sample' named entity 'tested' named entity 'detection' named entity 'samples' named entity 'Here' named entity 'SARS-CoV-2' named entity 'demonstrate' named entity 'particles' named entity 'EUA' named entity 'CDC' named entity 'SARS-CoV-2' named entity 'hydrogel' named entity 'RNA extraction' named entity 'transport medium' named entity 'viral load' named entity 'real-time RT-PCR' named entity 'saliva' named entity 'SARS-CoV-2' named entity 'SARS-CoV-2' named entity 'SARS-CoV-2' named entity '1,000,000' named entity 'SARS-CoV-2' named entity 'SARS-CoV-2' named entity 'SARS-CoV-2' named entity 'genetic material' named entity 'SARS-CoV-2' named entity 'milliliter' named entity 'RNA' named entity 'RNA' named entity 'SARS-CoV-2' named entity 'saliva' named entity 'SARS-CoV-2' named entity 'detergent' named entity 'milliliter' named entity 'virus' named entity 'freeze-thaw' named entity 'milliliter' named entity 'real-time RT-PCR' named entity 'viral RNA' named entity 'dry cough' named entity 'microliters'

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