About: Live-attenuated Salmonella enterica serotype Choleraesuis vaccine with regulated delayed fur mutation confer protection against Streptococcus suis in mice

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About: Live-attenuated Salmonella enterica serotype Choleraesuis vaccine with regulated delayed fur mutation confer protection against Streptococcus suis in mice Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Live-attenuated Salmonella enterica serotype Choleraesuis vaccine with regulated delayed fur mutation confer protection against Streptococcus suis in mice
Creator	Wang, Xiaobo Chen, Yunyun Guo, Weiwei Chu, Dianfeng Li, Yu-An Fan, Juan Bellefleur, Matthew Shi, Huoying Wang, Shifeng Zhao Du, Yuan
Source	PMC
abstract	BACKGROUND: Recombinant Salmonella enterica serotype Choleraesuis (S. Choleraesuis) vaccine vector could be used to deliver heterologous antigens to prevent and control pig diseases. We have previously shown that a live-attenuated S. Choleraesuis vaccine candidate strain rSC0011 (ΔP(crp527)::TT araC P(BAD)crp Δpmi-2426 ΔrelA199::araC P(BAD)lacI TT ΔasdA33, Δ, deletion, TT, terminator) delivering SaoA, a conserved surface protein in most of S. suis serotypes, provided excellent protection against S. suis challenge, but occasionally lead to morbidity (enteritidis) in vaccinated mice (approximately 1 in every 10 mice). Thus, alternated attenuation method was sought to reduce the reactogenicity of strain rSC0011. Herein, we described another recombinant attenuated S. Choleraesuis vector, rSC0012 (ΔP(fur88):: TT araC P(BAD)fur Δpmi-2426 ΔrelA199:: araC P(BAD)lacI TT ΔasdA33) with regulated delayed fur mutation to avoid inducing disease symptoms while exhibiting a high degree of immunogenicity. RESULTS: The strain rSC0012 strain with the ΔP(fur88)::TT araC P(BAD)fur mutation induced less production of inflammatory cytokines than strain rSC0011 with the ΔP(crp527)::TT araC P(BAD)crp mutation in mice. When delivering the same pS-SaoA plasmid, the intraperitoneal LD(50) of rSC0012 was 18.2 times higher than that of rSC0011 in 3-week-old BALB/C mice. rSC0012 with either pS-SaoA or pYA3493 was cleared from spleen and liver tissues 7 days earlier than rSC0011 with same vectors after oral inoculation. The strain rSC0012 synthesizing SaoA induced high titers of anti-SaoA antibodies in both systemic (IgG in serum) and mucosal (IgA in vaginal washes) sites, as well as increased level of IL-4, the facilitator of Th2-type T cell immune response in mice. The recombinant vaccine rSC0012(pS-SaoA) conferred high percentage of protection against S. suis or S. Choleraesuis challenge in BALB/C mice. CONCLUSIONS: The live-attenuated Salmonella enterica serotype Choleraesuis vaccine rSC0012(pS-SaoA) with regulated delayed fur mutation provides a foundation for the development of a safe and effective vaccine against S. Choleraesuis and S. suis.
has issue date	2020-05-07(xsd:dateTime)
bibo:doi	10.1186/s12917-020-02340-4
bibo:pmid	32381017
has license	cc-by
sha1sum (hex)	cc29327d0d6c287986ce95dace0072364860a858
schema:url	https://doi.org/10.1186/s12917-020-02340-4
resource representing a document's title	Live-attenuated Salmonella enterica serotype Choleraesuis vaccine with regulated delayed fur mutation confer protection against Streptococcus suis in mice
has PubMed Central identifier	PMC7203871
has PubMed identifier	32381017
schema:publication	BMC Vet Res
resource representing a document's body	covid:cc29327d0d6c287986ce95dace0072364860a858#body_text
is schema:about of	named entity 'IMMUNOGENICITY' named entity 'INTRAPERITONEAL' named entity 'MUCOSAL' named entity 'BUT' named entity 'INDUCED' named entity 'LEVEL' named entity 'IMMUNE RESPONSE' named entity 'SERUM' named entity 'ARAC' named entity 'REGULATED' named entity 'DISEASE' named entity 'PRODUCTION' named entity 'ANTIBODIES' named entity 'immune response' named entity 'mice' named entity 'mice' named entity 'mutation' named entity 'Recombinant' named entity 'pig' named entity 'protein' named entity 'IgA' named entity 'LD 50' named entity 'mucosal' named entity 'crp' named entity 'vector' named entity 'heterologous' named entity 'serotype' named entity 'recombinant' named entity 'recombinant vaccine' named entity 'Streptococcus suis' named entity 'virulent' named entity 'vaccine' named entity 'liver' named entity 'secretion' named entity 'Peyer's patch' named entity 'immunization' named entity 'live attenuated' named entity 'expression vector' named entity 'immunogenicity' named entity 'inoculation' named entity 'IL-6' named entity 'qPCR' named entity '0.01' named entity 'RNA' named entity 'vector' named entity 'immunization' named entity 'iron metabolism' named entity 'intracellular' named entity 'vaccine' named entity 'China' named entity 'spleen' named entity 'standard deviations' named entity 'live-attenuated' named entity 'protein' named entity 'transcriptional activator' named entity 'mutation' named entity 'IgG' named entity 'immunization' named entity 'IFN-γ' named entity 'inflammatory cytokine' named entity 'LPS' named entity 'NIH' named entity 'vaccines' named entity 'Veterinary Drug' named entity 'immunogenicity' named entity 'antigen' named entity 'S. Typhimurium' named entity 'liver' named entity 'primers' named entity 'mutation' named entity 'mice' named entity 'TNFα'

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