About: Quantification of Viral Inactivation by Photochemical Treatment with Amotosalen and UV A Light, Using a Novel Polymerase Chain Reaction Inhibition Method with Preamplification

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About: Quantification of Viral Inactivation by Photochemical Treatment with Amotosalen and UV A Light, Using a Novel Polymerase Chain Reaction Inhibition Method with Preamplification Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Quantification of Viral Inactivation by Photochemical Treatment with Amotosalen and UV A Light, Using a Novel Polymerase Chain Reaction Inhibition Method with Preamplification
Creator	Allain, Jean-Pierre Lin, Lily Corash, Laurence Fischetti, Lucia Hanson, Deborah Hsu, Jocelyn Pranmeth, Manisha Stassinopoulos, Adonis
source	PMC
abstract	Background. In evaluating a photochemical treatment process for inactivating parvovirus B19, there lacked simple culture methods to measure infectivity. The recently developed enzyme‐linked immunospot (ELISpot) infectivity assay uses late‐stage erythropoietic progenitor cells and is labor intensive and time consuming. We evaluated a novel, efficient polymerase chain reaction (PCR) inhibition assay and examined correlations with reductions in infectivity. Methods. Contaminated plasma was treated with 150 μmol/L amotosalen and 3 J/cm(2) ultraviolet A light and then tested for DNA modification using conventional PCR inhibition and a novel preamplification approach. The novel assay subjected the samples to preamplification cycles using long‐template PCR, followed by quantitative PCR (QPCR) inhibition detection. Both approaches were tested for correlations with reductions in viral infectivity by comparing ELISpot assay results of identical samples. Results. The B19 preamplification inhibition assay showed detection ranges of 2–2.5 log and demonstrated quantitative correlation with up to a 5.8‐log reduction in viral infectivity in ELISpot results. Conventional PCR detected a >5 log reduction in amplification, correlated with a 4.4‐log reduction in viral infectivity. A range of 4‐log inhibition of hepatitis B virus DNA amplification was also achieved. Conclusions. The results demonstrated that a novel preamplification QPCR assay is a useful tool for predicting reductions in infectivity after photochemical treatment. This assay was extended to show utility in circumstances where practical in vitro assays are unavailable for the determination of the efficacy of pathogen inactivation.
has issue date	2006-12-15(xsd:dateTime)
bibo:doi	10.1086/509260
bibo:pmid	17109347
has license	no-cc
sha1sum (hex)	cc3465002fe302cf36d80f712d200dc409e7cc52
schema:url	https://doi.org/10.1086/509260
resource representing a document's title	Quantification of Viral Inactivation by Photochemical Treatment with Amotosalen and UV A Light, Using a Novel Polymerase Chain Reaction Inhibition Method with Preamplification
has PubMed Central identifier	PMC7110026
has PubMed identifier	17109347
schema:publication	J Infect Dis
resource representing a document's body	covid:cc3465002fe302cf36d80f712d200dc409e7cc52#body_text
is schema:about of	named entity 'USING' named entity 'polymerase' named entity 'photochemical' named entity 'culture' named entity 'correlations' named entity 'PHOTOCHEMICAL' named entity 'INHIBITION' named entity 'ENZYME' named entity 'TIME' named entity 'EXAMINED' named entity 'INHIBITION' named entity 'LATE-STAGE' named entity 'CONSUMING' named entity 'NOVEL' named entity 'POLYMERASE CHAIN REACTION' named entity 'ASSAY' named entity 'CORRELATIONS' named entity 'TREATMENT' named entity 'RECENTLY' named entity 'EFFICIENT' named entity 'ERYTHROPOIETIC PROGENITOR CELLS' named entity 'PHOTOCHEMICAL' named entity 'INFECTIVITY' named entity 'LIGHT' named entity 'VIRAL INACTIVATION' named entity 'AMOTOSALEN' named entity 'POLYMERASE CHAIN REACTION' named entity 'METHOD' named entity 'QUANTIFICATION' named entity 'NOVEL' named entity 'REDUCTIONS' named entity 'LABOR' named entity 'METHODS' named entity 'EVALUATED' named entity 'PARVOVIRUS B19' named entity 'EVALUATING' named entity 'TREATMENT PROCESS' named entity 'SIMPLE' named entity 'TO MEASURE' named entity 'USES' named entity 'CULTURE' covid:arg/cc3465002fe302cf36d80f712d200dc409e7cc52 named entity 'B19' named entity 'assay' named entity 'intensive' named entity 'assay' named entity 'infectivity' named entity 'polymerase chain reaction' named entity 'assay' named entity 'culture methods' named entity 'enzyme' named entity 'viral titers' named entity 'amplicon' named entity 'intercalating' named entity 'Applied Biosystems' named entity 'PCR' named entity 'CD36' named entity 'QPCR' named entity 'nonenveloped virus' named entity 'QPCR' named entity 'Kumasi' named entity 'serially diluted' named entity 'dynamic range' named entity 'PCR' named entity 'primers' named entity 'photochemical' named entity 'Ghana' named entity 'PCR' named entity 'transfusion' named entity 'platelets' named entity 'transfusion' named entity 'titer' named entity 'blood transfusions' named entity 'QPCR'

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