About: Characterization of DNA hybridization kinetics in a microfluidic flow channel

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Characterization of DNA hybridization kinetics in a microfluidic flow channel
Creator	Jia, Xi-Yu Marafie, Alia Hyong, Joshua Kim, -Seok Madou, Marc Zoval, Jim
Source	Elsevier; Medline; PMC
abstract	Abstract In this investigation we report on the influence of volumetric flow rate, flow velocity, complementary DNA concentration, height of a microfluidic flow channel and time on DNA hybridization kinetics. A syringe pump was used to drive Cy3-labeled target DNA through a polydimethylsiloxane (PDMS) microfluidic flow channel to hybridize with immobilized DNA from the West Nile Virus. We demonstrate that a reduction of channel height, while keeping a fixed volumetric flow rate or a fixed flow velocity, enhances mass transport of target DNA to the capture probes. Compared to a passive hybridization, the DNA hybridization in the microfluidic flow channel generates higher fluorescence intensities for lower concentration of target DNA during the same fixed period of time. Within a fixed 2min time period the fastest DNA hybridization at a 50pM concentration of target DNA is achieved with a continuous flow of target DNA at the highest flow rate and the lowest channel height.
has issue date	2006-01-17(xsd:dateTime)
bibo:doi	10.1016/j.snb.2005.03.034
bibo:pmid	32288235
has license	els-covid
sha1sum (hex)	cdb216817dfba5ec74a92a34efdcb748595d0a9b
schema:url	https://doi.org/10.1016/j.snb.2005.03.034
resource representing a document's title	Characterization of DNA hybridization kinetics in a microfluidic flow channel
has PubMed Central identifier	PMC7125766
has PubMed identifier	32288235
schema:publication	Sensors and Actuators B: Chemical
resource representing a document's body	covid:cdb216817dfba5ec74a92a34efdcb748595d0a9b#body_text
is schema:about of	named entity 'microfluidic' named entity 'DNA hybridization' named entity 'probes' named entity 'drive' named entity 'polydimethylsiloxane' named entity 'DNA' named entity 'target' named entity 'capture' named entity 'demonstrate' named entity 'generates' named entity 'influence' named entity 'min' named entity 'syringe pump' named entity 'DNA' named entity 'microfluidic' named entity 'channel' named entity 'labeled' named entity 'concentration' named entity 'fixed' named entity 'hybridization' named entity 'volumetric flow rate' named entity 'fluorescence' named entity 'flow rate' named entity 'mass transport' named entity 'syringe pump' named entity 'flow velocity' named entity 'microfluidic' named entity 'concentration gradient' named entity 'oligonucleotides' named entity 'fluorescence' named entity 'Corona Virus' named entity 'centrifugation' named entity 'hybridization' named entity 'fixed target' named entity 'hybridization' named entity 'signal to noise ratio' named entity 'LDL' named entity 'master mold' named entity 'volumetric flow rate' named entity 'hybridization' named entity 'flow rates' named entity 'microfluidic' named entity 'micro-array' named entity 'microfluidic' named entity 'fluorescence intensity' named entity 'hybridization' named entity 'electric field' named entity 'hybridization' named entity 'micro-array analysis' named entity 'Cy3' named entity 'syringe pump' named entity 'flow rates' named entity 'deionized water' named entity 'convective transport' named entity 'DNA hybridization' named entity 'single stranded' named entity 'hybridization' named entity 'Peclet number' named entity 'microfluidic' named entity 'flow is laminar' named entity 'Peclet number' named entity 'electric field' named entity 'phosphate groups' named entity 'electrophoretic' named entity 'electrophoresis' named entity 'volumetric flow rate' named entity 'flow rate' named entity 'point mutations' named entity 'Reynolds number' named entity 'incubation time' named entity 'laser' named entity 'reaction rate' named entity 'buffer solution' named entity 'DNA hybridization' named entity 'molecule' named entity 'laminar'

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