About: Antibodies that potently inhibit or enhance SARS-CoV-2 spike protein-ACE2 interaction isolated from synthetic single-chain antibody libraries

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About: Antibodies that potently inhibit or enhance SARS-CoV-2 spike protein-ACE2 interaction isolated from synthetic single-chain antibody libraries Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Antibodies that potently inhibit or enhance SARS-CoV-2 spike protein-ACE2 interaction isolated from synthetic single-chain antibody libraries
Creator	Le, Thien-Kim Aracic, Sanja Beasley, Matthew Church, Nicole Gracey, Fiona Jabar, Shereen Kannan, Ruban Kiefel, Ben Makris, Dahna Masarati, Avisa Mccoll, Bradley Premaratne, S Udawela, Madhara Wood, Rebecca
Source	BioRxiv
abstract	Antibodies with high affinity against the receptor binding domain (RBD) of the SARS-CoV-2 S1 ectodomain were identified from screens using the Retained Display™ (ReD) platform employing a 1 × 1011 clone single-chain antibody (scFv) library. Numerous unique scFv clones capable of inhibiting binding of the viral S1 ectodomain to the ACE2 receptor in vitro were characterized. To maximize avidity, selected clones were reformatted as bivalent diabodies and monoclonal antibodies (mAb). The highest affinity mAb completely neutralized live SARS-CoV-2 virus in cell culture for four days at a concentration of 6.7 nM, suggesting potential therapeutic and/or prophylactic use. Furthermore, scFvs were identified that greatly increased the interaction of the viral S1 trimer with the ACE2 receptor, with potential implications for vaccine development.
has issue date	2020-07-28(xsd:dateTime)
bibo:doi	10.1101/2020.07.27.224089
has license	biorxiv
sha1sum (hex)	cddbdb9568bcba791ab5f45096257cba44feb364
schema:url	https://doi.org/10.1101/2020.07.27.224089
resource representing a document's title	Antibodies that potently inhibit or enhance SARS-CoV-2 spike protein-ACE2 interaction isolated from synthetic single-chain antibody libraries
schema:publication	bioRxiv
resource representing a document's body	covid:cddbdb9568bcba791ab5f45096257cba44feb364#body_text
is schema:about of	named entity 'ACE2' named entity 'UNIQUE' named entity 'SARS-CoV-2 virus' named entity 'scFv' named entity 'avidity' named entity 'ectodomain' named entity '7 nM' named entity 'monoclonal antibodies' named entity 'spike protein' named entity 'BLI' named entity 'neutralizing antibodies' named entity 'protein' named entity 'ectodomain' named entity 'antibody' named entity 'biotin' named entity 'molar concentration' named entity 'ACE2' named entity 'SARS' named entity 'SARS-CoV-2' named entity 'RBD' named entity 'BLI' named entity 'cytoplasm' named entity 'BLI' named entity 'ACE2' named entity 'COVID' named entity 'scFv' named entity 'developing world' named entity '0.9' named entity 'ACE2' named entity 'antibody-dependent enhancement' named entity 'nM 22' named entity 'recombinant' named entity 'diabodies' named entity 'SARS-CoV-2' named entity 'infectivity' named entity 'fluorescence' named entity 'ACE2' named entity 'vaccine' named entity 'ACE2' named entity 'protein' named entity 'MERS-CoV' named entity 'high affinity' named entity '14 nM' named entity 'SARS-CoV-2' named entity 'FACS' named entity 'scFv' named entity 'antibody' named entity 'molar' named entity 'infection' named entity 'ACE2' named entity 'virus' named entity 'Antibody' named entity 'SARS-CoV-2' named entity 'long-term' named entity 'fluorescence' named entity 'bio-layer interferometry' named entity 'protein' named entity 'infection' named entity 'scFvs' named entity 'ACE2' named entity 'scFvs' named entity 'ACE2' named entity 'health care workers' named entity 'viruses' named entity 'mAb' named entity 'variable domain' named entity 'antibody' named entity 'clone' named entity 'Antibody-mediated' named entity 'FACS' named entity 'BLI'

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