About: SARS-CoV-2 detection with CRISPR diagnostics

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: SARS-CoV-2 detection with CRISPR diagnostics Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	SARS-CoV-2 detection with CRISPR diagnostics
Creator	Li, Wei Liang, Chen Zhou, Qi Wang, Liu Chen, Yangcan Dai, Moyu Fang, Sen Feng, Guihai Gao, Qingqin Gu, Qi Guo, Lu Jiang, Haiping Mao, Yihuan Qu, Bin Sun, Xuehan Wang, Ruiqi Wang, Xinge
Source	BioRxiv
abstract	The novel coronavirus (CoV) disease termed COVID-19 (Coronavirus Disease-19) caused by SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus-2) is causing a massive pandemic worldwide, threatening public health systems across the globe. During this ongoing COVID-19 outbreak, nucleic acid detection has played an important role in early diagnosis. Here we report a SARS-CoV-2 detection protocol using a CRISPR-based CRISPR diagnostic platform - CDetection (Cas12b-mediated DNA detection). By combining sample treatment protocols and nucleic acid amplification methods with CDetection, we have established an integrated viral nucleic acid detection platform - CASdetec (CRISPR-assisted detection). The detection limit of CASdetec for SARS-CoV-2 pseudovirus is 1 × 104 copies/mL, with no cross reactivity observed. Our assay design and optimization process can provide guidance for future CRISPR-based nucleic acid detection assay development and optimization.
has issue date	2020-04-11(xsd:dateTime)
bibo:doi	10.1101/2020.04.10.023358
has license	biorxiv
sha1sum (hex)	cdf2bb810fd33f983b42c461eabae8e4472ec434
schema:url	https://doi.org/10.1101/2020.04.10.023358
resource representing a document's title	SARS-CoV-2 detection with CRISPR diagnostics
schema:publication	bioRxiv
resource representing a document's body	covid:cdf2bb810fd33f983b42c461eabae8e4472ec434#body_text
is schema:about of	named entity 'disease' named entity 'CRISPR' named entity 'NOVEL CORONAVIRUS' named entity 'World Health Organization' named entity 'fluorescence' named entity 'POCT' named entity 'signal-to-background ratio' named entity 'pseudovirus' named entity 'detection limit' named entity 'COVID-19 outbreak' named entity 'SARS-CoV' named entity 'CRISPR' named entity '10 nM' named entity 'nucleic acid detection' named entity 'SARS-CoV-2' named entity 'fluorescence' named entity 'CRISPR' named entity 'lysis buffer' named entity 'nucleic acid detection' named entity 'nucleic acid amplification' named entity 'primers' named entity 'coronaviruses' named entity 'nucleic acid detection' named entity 'coronavirus' named entity 'RNA' named entity 'detection limit' named entity 'fluorescence' named entity 'CRISPR' named entity 'microliter' named entity 'fluorescence' named entity 'lentivirus' named entity 'endemic' named entity 'CRISPR' named entity 'SARS-CoV-2' named entity 'assay' named entity 'CRISPR' named entity 'coronavirus' named entity 'detection limit' named entity 'CRISPR' named entity 'lysis buffer' named entity 'assay' named entity 'public health' named entity 'COVID-19 outbreak' named entity 'pseudovirus' named entity 'RdRp' named entity 'point-of-care testing' named entity 'MERS-CoV' named entity 'SARS' named entity 'SARS-CoV' named entity 'nucleotide' named entity 'cross-reactivity' named entity 'primers' named entity 'RNA' named entity 'Severe Acute Respiratory Syndrome Coronavirus' named entity 'CRISPR' named entity 'SARS-CoV' named entity 'lysis buffer' named entity 'assay' named entity 'nucleic acid amplification' named entity 'SARS-CoV-2' named entity 'sensitivity and specificity' named entity 'quenchers' named entity 'SARS-CoV-2' named entity 'Institute of Zoology' named entity 'CRISPR' named entity 'coronaviruses' named entity 'WHO' named entity 'fluorescence'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software