About: Ultra-fast one-step RT-PCR protocol for the detection of SARS-CoV-2

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Ultra-fast one-step RT-PCR protocol for the detection of SARS-CoV-2
Creator	Knabbe, Cornelius Vollmer, Tanja Asghari, Ehsan De Vos, Gert Höving, Anna Kaltschmidt, Barbara Kiel, Annika Krahlemann-Köhler, Angela Lütkemeyer, Melanie Storm, Jonathan Van Heijningen, Paula
source	MedRxiv
abstract	The COVID-19 pandemic resulted in lockdowns all over the world thus affecting nearly all aspects of social life and also had a huge impact on global economies. Since vaccines and therapies are still not available for the population, prevention becomes desperately needed. One important aspect for prevention is the identification and subsequent isolation of contagious specimens. The currently used methods for diagnostics are time consuming and also hindered by the limited availability of reagents and reaction costs, thus presenting a bottle neck for prevention of COVID-19 spread. Here, we present a new ultra-fast test method which is ten times faster than conventional diagnostic tests using real time quantitative PCR (RT-qPCR). In addition, this ultra-fast method is easy to handle as well as cost effective. We translated published SARS-CoV-2 testing protocols from the Centers of Disease Control and Prevention (Atlanta, Georgia, USA) and the Charite Berlin (Germany) to the NEXTGENPCR (NGPCR) machine and combined it with a fluorescence-based endpoint measurement. Fluorescence was measured with a commercial blue light scanner. We confirmed the NEXTGENPCR results with commercially available positive controls. In addition, we isolated RNA from SARS-CoV-2 infected patients and achieved similar results to clinical RT-qPCR assays. Here, we could show correlation between the results obtained by NEXTGENPCR and conventional RT-qPCR.
has issue date	2020-06-26(xsd:dateTime)
bibo:doi	10.1101/2020.06.25.20137398
has license	medrxiv
sha1sum (hex)	cf3c5953c885bfe383a7a9b5257b3300f5c24e90
schema:url	https://doi.org/10.1101/2020.06.25.20137398
resource representing a document's title	Ultra-fast one-step RT-PCR protocol for the detection of SARS-CoV-2
resource representing a document's body	covid:cf3c5953c885bfe383a7a9b5257b3300f5c24e90#body_text
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