About: Mutations from bat ACE2 orthologs markedly enhance ACE2-Fc neutralization of SARS-CoV-2

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About: Mutations from bat ACE2 orthologs markedly enhance ACE2-Fc neutralization of SARS-CoV-2 Goto Sponge NotDistinct Permalink

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Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Mutations from bat ACE2 orthologs markedly enhance ACE2-Fc neutralization of SARS-CoV-2
Creator	Guo, Yan Farzan, Michael Choe, Hyeryun Mou, Huihui Bailey, Charles Rader, Christoph Zhang, Lizhou Alpert, Michael Crynen, Gogce Davis-Gardner, Meredith Gardner, Matthew Peng, Haiyong Peng, Shoujiao Quinlan, Brian Voo, Zhi
Source	BioRxiv; Medline; PMC
abstract	The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) protein mediates infection of cells expressing angiotensin-converting enzyme 2 (ACE2). ACE2 is also the viral receptor of SARS-CoV (SARS-CoV-1), a related coronavirus that emerged in 2002–2003. Horseshoe bats (genus Rhinolophus) are presumed to be the original reservoir of both viruses, and a SARS-like coronavirus, RaTG13, closely related SARS-CoV-2, has been isolated from one horseshoe-bat species. Here we characterize the ability of S-protein receptor-binding domains (RBDs) of SARS-CoV-1, SARS-CoV-2, and RaTG13 to bind a range of ACE2 orthologs. We observed that the SARS-CoV-2 RBD bound human, pangolin, and horseshoe bat (R. macrotis) ACE2 more efficiently than the SARS-CoV-1 or RaTG13 RBD. Only the RaTG13 RBD bound rodent ACE2 orthologs efficiently. Five mutations drawn from ACE2 orthologs of nine Rhinolophus species enhanced human ACE2 binding to the SARS-CoV-2 RBD and neutralization of SARS-CoV-2 by an immunoadhesin form of human ACE2 (ACE2-Fc). Two of these mutations impaired neutralization of SARS-CoV-1. An ACE2-Fc variant bearing all five mutations neutralized SARS-CoV-2 five-fold more efficiently than human ACE2-Fc. These data narrow the potential SARS-CoV-2 reservoir, suggest that SARS-CoV-1 and −2 originate from distinct bat species, and identify a more potently neutralizing form of ACE2-Fc.
has issue date	2020-06-30(xsd:dateTime)
bibo:doi	10.1101/2020.06.29.178459
bibo:pmid	32637954
has license	cc-by-nc-nd
sha1sum (hex)	cf80a18de5e8b8099e1aef0f3f7af84e9aced5e4
schema:url	https://doi.org/10.1101/2020.06.29.178459
resource representing a document's title	Mutations from bat ACE2 orthologs markedly enhance ACE2-Fc neutralization of SARS-CoV-2
has PubMed Central identifier	PMC7337384
has PubMed identifier	32637954
schema:publication	bioRxiv
resource representing a document's body	covid:cf80a18de5e8b8099e1aef0f3f7af84e9aced5e4#body_text
is schema:about of	named entity 'SARS-CoV' named entity 'SARS-CoV-2' named entity 'SARS-CoV-2' named entity 'SARS-CoV-2' named entity 'ACE2' named entity 'ACE2' named entity 'binding' named entity 'neutralization' named entity 'SARS-CoV-2' named entity 'orthologs' named entity 'RBD' named entity 'neutralizing' named entity 'neutralization' named entity 'enhanced' named entity 'coronavirus' named entity 'viruses' named entity 'range' named entity 'SARS-CoV-1' named entity 'efficiently' named entity 'SARS-like coronavirus' named entity 'bound' named entity 'genus' named entity 'RBD' named entity 'horseshoe bat' named entity 'ACE2' named entity 'bat species' named entity 'ACE2' named entity 'Horseshoe bats' named entity 'horseshoe bats' named entity 'RBD' named entity 'SARS-CoV-2' named entity 'SARS-CoV-1' named entity 'viruses' named entity 'SARS-CoV-2' named entity 'orthologs' named entity 'genus' named entity 'ACE2' named entity 'SARS-CoV-2' named entity 'SARS-CoV-1' named entity 'coronavirus' named entity 'ACE2' named entity 'Rhinolophus' named entity 'SARS-CoV' named entity 'orthologs' named entity 'bound' named entity 'bat' named entity 'orthologs' named entity 'viral membrane' named entity 'serine' named entity 'RBD' named entity 'CC-BY-NC-ND 4.0' named entity '293T' named entity 'amino-acids' named entity 'initial infection' named entity 'receptor' named entity 'virus' named entity 'flow cytometry' named entity 'pangolin' named entity 'Wrapp' named entity 'orthologs' named entity 'incubation' named entity 'SARS2' named entity 'bat species' named entity 'ACE2' named entity 'pangolins' named entity 'protein' named entity 'IgG1'

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