About: Optimization of Codon Usage of Poxvirus Genes allows for Improved Transient Expression in Mammalian Cells

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About: Optimization of Codon Usage of Poxvirus Genes allows for Improved Transient Expression in Mammalian Cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Optimization of Codon Usage of Poxvirus Genes allows for Improved Transient Expression in Mammalian Cells
Creator	Barrett, John Mcfadden, Grant Ae, Craig Ae, Steven Ae, Sun Ae, Tara Barrett, J Belsito, Ae Brunetti, C Mcfadden, Ae Nazarian, Ae Sun, Ae Yunming, A
Source	Medline; PMC
abstract	Transient expression of viral genes from certain poxviruses in uninfected mammalian cells can sometimes be unexpectedly inefficient. The reasons for poor expression levels can be due to a number of features of the gene cassette, such as cryptic splice sites, polymerase II termination sequences or motifs that lead to mRNA instability. Here we suggest that in some cases the problem of low protein expression in transfected mammalian cells may be due to inefficient codon usage. We have observed that for many poxvirus genes from the yatapoxvirus genus this deficiency can be overcome by synthesis of the gene with codon sequences optimized for expression in primate cells. This led us to examine colon usage across 2-dozen sequenced members of the Poxviridae. We conclude that codon usage is surprisingly divergent across the different Poxviridae genera but is much more conserved within a single genus. Thus, Poxviridae genera can be divided into distinct groups based on their observed codon bias. When viewed in this context, successful transient expression of transfected poxvirus genes in uninfected mammalian cells can be more accurately predicted based on codon bias. As a corollary, for specific poxvirus genes with less favorable codon usage, codon optimization can result in profoundly increased transient expression levels following transfection of uninfected mammalian cell lines.
has issue date	2006-01-01(xsd:dateTime)
bibo:doi	10.1007/s11262-005-0035-7
bibo:pmid	16791414
has license	no-cc
sha1sum (hex)	d09ecfd5cef1bcb5dff8519888d9064a85dc71e3
schema:url	https://doi.org/10.1007/s11262-005-0035-7
resource representing a document's title	Optimization of Codon Usage of Poxvirus Genes allows for Improved Transient Expression in Mammalian Cells
has PubMed Central identifier	PMC7089053
has PubMed identifier	16791414
schema:publication	Virus Genes
resource representing a document's body	covid:d09ecfd5cef1bcb5dff8519888d9064a85dc71e3#body_text
is schema:about of	named entity 'problem' named entity 'genes' named entity 'expression' named entity 'observed' named entity 'lead' named entity 'optimized' named entity 'groups' named entity 'CODON USAGE' named entity 'POXVIRUS' named entity 'TO EXAMINE' named entity 'POXVIRUSES' named entity 'LOW PROTEIN' named entity 'CONSERVED' named entity 'DIFFERENT' named entity 'MRNA INSTABILITY' named entity 'HAVE' named entity 'GENES' named entity 'DUE TO' named entity 'CELLS' named entity 'SPECIFIC' named entity 'EXPRESSION' named entity 'CODON BIAS' named entity 'MEMBERS' named entity 'PRIMATE' named entity 'COLON' named entity 'TRANSFECTION' named entity 'VIRAL GENES' named entity 'MAMMALIAN CELL' named entity 'MAY BE' named entity 'GENE CASSETTE' named entity 'PROBLEM' named entity 'UNINFECTED' named entity 'TRANSIENT EXPRESSION' named entity 'YATAPOXVIRUS' named entity 'FOLLOWING' named entity 'GENES' named entity 'PROTEIN EXPRESSION' named entity 'GENE' named entity 'IMPROVED' named entity 'TRANSIENT EXPRESSION' named entity 'MAMMALIAN' named entity 'ALLOWS' named entity 'OPTIMIZATION' named entity 'DEFICIENCY' named entity 'POXVIRUS' named entity 'CERTAIN' named entity 'MUCH' named entity 'BUT' named entity 'USAGE' named entity 'NUMBER OF' named entity 'GROUPS' named entity 'TERMINATION' named entity 'LED' named entity 'CONTEXT' named entity 'HERE' named entity 'MAMMALIAN' named entity 'POLYMERASE' named entity 'RESULT' named entity 'CODON USAGE' named entity 'DISTINCT' named entity 'SYNTHESIS' named entity 'FEATURES' named entity 'CODON' named entity 'POXVIRIDAE' named entity 'THEIR' named entity 'LEVELS' named entity 'ACROSS' named entity 'INCREASED' named entity 'CELLS'

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