About: Detection limit of negative staining electron microscopy for the diagnosis of bioterrorism‐related micro‐organisms

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About: Detection limit of negative staining electron microscopy for the diagnosis of bioterrorism‐related micro‐organisms Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Detection limit of negative staining electron microscopy for the diagnosis of bioterrorism‐related micro‐organisms
Creator	Bannert, N Laue, M
source	Medline; PMC
abstract	Aims: To determine the detection limit of diagnostic negative staining electron microscopy for the diagnosis of pathogens that could be used for bioterrorism. Methods and Results: Suspensions of vaccinia poxvirus and endospores of Bacillus subtilis were used at defined concentrations as a model for poxviruses and spores of anthrax (Bacillus anthracis), both of which are pathogens that could be used for bioterrorist attacks. Negative staining electron microscopy was performed directly or after sedimentation of these suspensions on to the sample supports using airfuge ultracentrifugation. For both virus and spores, the detection limit using direct adsorption of a 10‐μl sample volume onto the sample support was 10(6) particles per ml. Using airfuge ultracentrifugation with a sample volume of 80 μl, the detection limit could be reduced to 10(5) particles per ml for spores and to 5 × 10(4) particles per ml for poxviruses. The influence on particle detection of incubation time, washing and adsorption procedures was investigated. Conclusions: The reproducibility and sensitivity of the method were acceptable, particularly considering the small sample volume and low particle number applied onto the sample support. Significance and Impact of the Study: Diagnostic negative staining electron microscopy is used for the diagnosis of pathogens in emergency situations because it allows a rapid examination of all particulate matter down to the nanometre scale. This study provides precise detection limit for the method, an important factor for the validation and improvement of the technique.
has issue date	2010-04-01(xsd:dateTime)
bibo:doi	10.1111/j.1365-2672.2010.04737.x
bibo:pmid	20456527
has license	no-cc
sha1sum (hex)	d0e881a9cba2fb7383be08fb2549750ae3abf920
schema:url	https://doi.org/10.1111/j.1365-2672.2010.04737.x
resource representing a document's title	Detection limit of negative staining electron microscopy for the diagnosis of bioterrorism‐related micro‐organisms
has PubMed Central identifier	PMC7197746
has PubMed identifier	20456527
schema:publication	J Appl Microbiol
resource representing a document's body	covid:d0e881a9cba2fb7383be08fb2549750ae3abf920#body_text
is schema:about of	named entity 'poxvirus' named entity 'bioterrorist' named entity 'sedimentation' named entity 'poxviruses' named entity 'sample' named entity 'nanometre' named entity 'For' named entity 'rapid' named entity 'Impact' named entity 'sample' named entity 'bioterrorism' named entity 'diagnosis' named entity 'Detection limit' named entity 'FACTOR' named entity 'SENSITIVITY' named entity 'VALIDATION' named entity 'detection limit' named entity 'ultracentrifugation' named entity 'supports' named entity 'procedures' named entity 'ultracentrifugation' named entity 'pathogens' named entity 'acceptable' named entity 'incubation time' named entity 'validation' named entity 'support' named entity 'precise' named entity 'diagnosis' named entity 'model' named entity 'ultracentrifugation' named entity 'Bacillus anthracis' named entity 'Negative staining' named entity 'bioterrorist' named entity 'Bacillus subtilis' named entity 'adsorption' named entity 'small sample' named entity 'nanometre' named entity 'poxviruses' named entity 'anthrax' named entity 'negative staining' named entity 'electron microscopy' named entity 'direct' named entity 'negative staining' named entity 'poxviruses' named entity 'spores' named entity 'alcian blue' named entity 'paraformaldehyde' named entity 'virus' named entity 'neutral pH' named entity 'virus' named entity 'Sigma-Aldrich' named entity 'detection limit' named entity 'particle number' named entity 'particle numbers' named entity 'Germany' named entity 'negative staining' named entity 'detection limit' named entity 'Fluka' named entity 'spores' named entity 'Particle numbers' named entity 'detection limit' named entity 'gravity' named entity 'particulate' named entity 'molecular weight' named entity 'reproducibility' named entity 'spores' named entity 'poxviruses' named entity 'ultracentrifugation' named entity 'SARS' named entity 'morphological' named entity 'detection limits' named entity 'spores' named entity 'spores' named entity 'alcian blue' named entity 'virus' named entity 'Detection limit' named entity 'anthrax spores' named entity 'copycat attacks' named entity 'nucleic acid amplification' named entity 'centrifugation'

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