About: Establishment of a panel of in‐house polyclonal antibodies for the diagnosis of enterovirus infections

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About: Establishment of a panel of in‐house polyclonal antibodies for the diagnosis of enterovirus infections Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Establishment of a panel of in‐house polyclonal antibodies for the diagnosis of enterovirus infections
Creator	Sata, Tetsutaro Nagata, Noriyo Taguchi, Fumihiro Hasegawa, Hideki Iwata-Yoshikawa, Naoko Kotani, Osamu Sato, Yuko Suzuki, Tadaki Shimizu, Hiroyuki Nakajima, Noriko Iwasaki, Takuya Koike, Satoshi Minagawa, Hiroko Yamashita, Teruo
Source	Medline; PMC
abstract	The aim of this study was to establish a reliable method of virus detection for the diagnosis of critical enterovirus infections such as acute infective encephalitis, encephalomyelitis and myocarditis. Because histopathological and immunohistochemical analyses of paraffin‐embedded tissues play an important role in recognizing infectious agents in tissue samples, six in‐house polyclonal antibodies raised against three representative enteroviruses using an indirect immunofluorescence assay and immunohistochemistry were examined. This panel of polyclonal antibodies recognized three serotypes of enterovirus. Two of the polyclonal antibodies were raised against denatured virus particles from enterovirus A71, one was raised against the recombinant VP1 protein of coxsackievirus B3, and the other for poliovirus type 1 were raised against denatured virus particles, the recombinant VP1 protein and peptide 2C. Western blot analysis revealed that each of these antibodies recognized the corresponding viral antigen and none cross‐reacted with non‐enteroviruses within the family Picornaviridae. However, all cross‐reacted to some extent with the antigens derived from other serotypes of enterovirus. Indirect immunofluorescence assay and immunohistochemistry revealed that the virus capsid and non‐structural proteins were localized in the cytoplasm of affected culture cells, and skeletal muscles and neurons in neonatal mice experimentally‐infected with human enterovirus. The antibodies also recognized antigens derived from recent clinical isolates of enterovirus A71, coxsackievirus B3 and poliovirus. In addition, immunohistochemistry revealed that representative antibodies tested showed the same recognition pattern according to each serotype. Thus, the panel of in‐house anti‐enterovirus polyclonal antibodies described herein will be an important tool for the screening and pathological diagnosis for enterovirus infections, and may be useful for the classification of different enterovirus serotypes, including coxsackieviruses A and B, echoviruses, enterovirus A71 and poliovirus.
has issue date	2014-09-28(xsd:dateTime)
bibo:doi	10.1111/neup.12171
bibo:pmid	25263613
has license	no-cc
sha1sum (hex)	d102f82ece520fcf2bb80fad357ce7b0fd886dbd
schema:url	https://doi.org/10.1111/neup.12171
resource representing a document's title	Establishment of a panel of in‐house polyclonal antibodies for the diagnosis of enterovirus infections
has PubMed Central identifier	PMC7168124
has PubMed identifier	25263613
schema:publication	Neuropathology
resource representing a document's body	covid:d102f82ece520fcf2bb80fad357ce7b0fd886dbd#body_text
is schema:about of	named entity 'play' named entity 'enterovirus' named entity 'assay' named entity 'enterovirus' named entity 'protein' named entity 'immunohistochemical' named entity 'A71' named entity 'However' named entity 'VP1' named entity 'aim' named entity 'denatured' named entity 'denatured' named entity 'serotypes' named entity 'panel' named entity 'Establishment' named entity 'SEROTYPE' named entity 'TOOL' named entity 'INFECTIOUS AGENTS' named entity 'METHOD' named entity 'THESE' named entity 'ISOLATES' named entity 'USEFUL' named entity 'INCLUDING' named entity 'PEPTIDE' named entity 'INDIRECT IMMUNOFLUORESCENCE' named entity 'TO SOME EXTENT' named entity 'ENTEROVIRUS INFECTIONS' named entity 'SEROTYPES' named entity 'PATTERN' named entity 'SCREENING' named entity 'LOCALIZED' named entity 'POLIOVIRUS' named entity 'MICE' named entity 'DIFFERENT' named entity 'CROSS' named entity 'DERIVED' named entity 'IMMUNOHISTOCHEMISTRY' named entity 'INFECTIVE ENCEPHALITIS' named entity 'A71' named entity 'diagnosis' named entity 'poliovirus' named entity 'recombinant' named entity 'samples' named entity 'family' named entity 'serotype' named entity 'raised' named entity 'immunohistochemistry' named entity 'recombinant' named entity 'Indirect immunofluorescence' named entity 'coxsackievirus' named entity 'tissues' named entity 'representative' named entity 'enteroviruses' named entity 'serotypes' named entity 'denatured' named entity 'immunohistochemical' named entity 'antibodies' named entity 'virus' named entity 'enterovirus A71' named entity 'enterovirus infections' named entity 'enterovirus infections' named entity 'antigens' named entity 'polyclonal antibodies' named entity 'type 1' named entity 'serotypes' named entity 'enterovirus A71' named entity 'polyclonal antibodies' named entity 'non-structural proteins'

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