About: Molecular cloning, expression, purification, and mass spectrometric characterization of 3C-like protease of SARS coronavirus

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About: Molecular cloning, expression, purification, and mass spectrometric characterization of 3C-like protease of SARS coronavirus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Molecular cloning, expression, purification, and mass spectrometric characterization of 3C-like protease of SARS coronavirus
Creator	Jiang, Hualiang Chen, Jing Chen, Kaixian Luo, Haibin Peng, Shuying Qin, Jun Shen, Jianhua Shen, Xu Sun, Haifang Sun, Tao Wang, Yuan Xie, Youhua Yang, Yiming Yu, Changying Jiang, H
source	Elsevier; Medline; PMC
abstract	Abstract Severe acute respiratory syndrome (SARS) is an acute respiratory illness, which has broken out in China. It has been known that SARS coronavirus (SARS_CoV) is a novel human coronavirus and is responsible for SARS infection. Belonging to one of the major proteins associated with SARS_CoV, SARS 3C-like protease (SARS_3CLpro) functions as a cysteine protease engaging in the proteolytic cleavage of the viral precursor polyprotein to a series of functional proteins required for coronavirus replication and is considered as an appealing target for designing anti-SARS agents. To facilitate the studies regarding the functions and structures of SARS_3CLpro, in this report the synthetic genes encoding 3CLpro of SARS_CoV were assembled, and the plasmid was constructed using pQE30 as vector and expressed in Escherichia coli M15 cells. The highly yielded (∼15mg/L) expressed protease was purified by use of NTA-Ni2+ affinity chromatography and FPLC system, and its sequence was determined by LC/MS with the residue coverage of 46.4%.
has issue date	2003-12-31(xsd:dateTime)
bibo:doi	10.1016/j.pep.2003.08.016
bibo:pmid	14965777
has license	els-covid
sha1sum (hex)	d10f869bf385bac400eca04356901f0647318818
schema:url	https://doi.org/10.1016/j.pep.2003.08.016
resource representing a document's title	Molecular cloning, expression, purification, and mass spectrometric characterization of 3C-like protease of SARS coronavirus
has PubMed Central identifier	PMC7129208
has PubMed identifier	14965777
schema:publication	Protein Expression and Purification
resource representing a document's body	covid:d10f869bf385bac400eca04356901f0647318818#body_text
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