About: Functional characterization of the partially purified Sac1p independent adenine nucleotide transport system (ANTS) from yeast endoplasmic reticulum

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About: Functional characterization of the partially purified Sac1p independent adenine nucleotide transport system (ANTS) from yeast endoplasmic reticulum Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Functional characterization of the partially purified Sac1p independent adenine nucleotide transport system (ANTS) from yeast endoplasmic reticulum
Creator	Li, Yuan Fiermonte, Giuseppe Aiello, Donatella Capobianco, Loredana Cappello, Anna Curcio, Rosita Dolce, Vincenza Frattaruolo, Luca Lappano, Rosamaria Lunetti, Paola Madeo, Marianna Maggiolini, Marcello Malivindi, Rocco Martello, Emanuela Muto, Luigina Piazzolla, Carmela Raho, Susanna Zaffino, Francesco
Source	PMC
abstract	Several ATP-depending reactions take place in the endoplasmic reticulum (ER). Although in Saccharomyces cerevisiae ER the existence of a Sac1p-dependent ATP transport system was already known, its direct involvement in ATP transport was excluded. Here we report an extensive biochemical characterization of a partially purified adenine nucleotide transport system (ANTS) not dependent on Sac1p. Highly purified ER membranes from the wild-type and Δsac1 yeast strains reconstituted into liposomes transported ATP with the same efficiency. A chromatography on hydroxyapatite was used to partially purify ANTS from Δsac1 ER extract. The two ANTS-enriched transport activity eluted fractions showed essentially the presence of four bands, one having an apparent MW of 56 kDa, similar to that observed for ANTS identified in rat liver ER. The two fractions reconstituted into liposomes efficiently transported, by a strict counter-exchange mechanism, ATP and ADP. ATP transport was saturable with a Km of 0.28 mM. The ATP/ADP exchange mechanism and the kinetic constants suggest that the main physiological role of ANTS is to catalyse the transport of ATP into ER, where it is used in several energy-requiring reactions and to export back to the cytosol the ADP produced.
has issue date	2018-06-08(xsd:dateTime)
bibo:doi	10.1093/jb/mvy054
bibo:pmid	29893873
has license	no-cc
sha1sum (hex)	d15e6dd20a34178a9cfe948184c504ea8d218aad
schema:url	https://doi.org/10.1093/jb/mvy054
resource representing a document's title	Functional characterization of the partially purified Sac1p independent adenine nucleotide transport system (ANTS) from yeast endoplasmic reticulum
has PubMed Central identifier	PMC7109914
has PubMed identifier	29893873
schema:publication	J Biochem
resource representing a document's body	covid:d15e6dd20a34178a9cfe948184c504ea8d218aad#body_text
is schema:about of	named entity 'fractions' named entity 'catalyse' named entity 'ATP' named entity 'eluted' named entity 'reactions' named entity 'fractions' named entity 'biochemical' named entity 'ATP' named entity 'ATP' named entity 'transport system' named entity 'TRANSPORT SYSTEM' named entity 'ADENINE NUCLEOTIDE TRANSPORT' named entity 'ENDOPLASMIC RETICULUM' named entity '100%' named entity 'CHARACTERIZATION' named entity 'INVOLVEMENT' named entity 'ATP TRANSPORT' named entity 'MEMBRANES' named entity 'RAT LIVER' named entity 'STRAINS' named entity 'SMOOTH ER' named entity 'EXTRACT' named entity 'TRANSPORT SYSTEM' named entity 'PARTIALLY PURIFIED' named entity 'ENRICHED' named entity 'EFFICIENCY' named entity 'OBSERVED' named entity 'ADENINE NUCLEOTIDE TRANSPORT' named entity 'MECHANISM' named entity 'KINETIC' named entity 'COUNTER' named entity 'SER' named entity 'WILD-TYPE' named entity 'HYDROXYAPATITE' named entity 'LIPOSOMES' named entity 'CATALYSE' named entity 'TO EXPORT' named entity 'PARTIALLY PURIFIED' named entity 'CHROMATOGRAPHY' named entity 'FRACTIONS' named entity 'TRANSPORT' named entity 'PHOSPHATE' named entity 'ACTIVITY' named entity 'SAC1P' named entity 'PARTIALLY' named entity 'ENDOPLASMIC RETICULUM' named entity 'SAC1P' named entity 'SIMILAR' named entity 'CYTOSOL' named entity '0.28' named entity 'PRESENCE OF' named entity 'YEAST' named entity 'HERE' named entity 'RER' named entity 'HIGHLY' named entity 'TRITON X-100' named entity 'INDEPENDENT' named entity 'FUNCTIONAL' named entity 'TX-100' named entity 'KNOWN' named entity 'PURIFIED' named entity 'TAKE PLACE' named entity 'EXCLUDED' named entity 'KDA'

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