About: Chikungunya triggers an autophagic process which promotes viral replication

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Chikungunya triggers an autophagic process which promotes viral replication Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Chikungunya triggers an autophagic process which promotes viral replication
Creator	Briant, Laurence Denizot, Mélanie Gasque, Philippe Gay, Bernard Hoarau, Jean-Jacques Jaffar-Bandjee, Marie-Christine Krejbich-Trotot, Pascale Li-Pat-Yuen, Ghislaine
Source	Medline; PMC
abstract	BACKGROUND: Chikungunya Virus (ChikV) surprised by a massive re-emerging outbreak in Indian Ocean in 2006, reaching Europe in 2007 and exhibited exceptional severe physiopathology in infants and elderly patients. In this context, it is important to analyze the innate immune host responses triggered against ChikV. Autophagy has been shown to be an important component of the innate immune response and is involved in host defense elimination of different pathogens. However, the autophagic process was recently observed to be hijacked by virus for their own replication. Here we provide the first evidence that hallmarks of autophagy are specifically found in HEK.293 infected cells and are involved in ChikV replication. METHODS: To test the capacity of ChikV to mobilize the autophagic machinery, we performed fluorescence microscopy experiments on HEK.GFP.LC3 stable cells, and followed the LC3 distribution during the time course of ChikV infection. To confirm this, we performed electron microscopy on HEK.293 infected cells. To test the effect of ChikV-induced-autophagy on viral replication, we blocked the autophagic process, either by pharmacological (3-MA) or genetic inhibition (siRNA against the transcript of Beclin 1, an autophagic protein), and analyzed the percentage of infected cells and the viral RNA load released in the supernatant. Moreover, the effect of induction of autophagy by Rapamycin on viral replication was tested. RESULTS: The increasing number of GFP-LC3 positive cells with a punctate staining together with the enhanced number of GFP-LC3 dots per cell showed that ChikV triggered an autophagic process in HEK.293 infected cells. Those results were confirmed by electron microscopy analysis since numerous membrane-bound vacuoles characteristic of autophagosomes were observed in infected cells. Moreover, we found that inhibition of autophagy, either by biochemical reagent and RNA interference, dramatically decreases ChikV replication. CONCLUSIONS: Taken together, our results suggest that autophagy may play a promoting role in ChikV replication. Investigating in details the relationship between autophagy and viral replication will greatly improve our knowledge of the pathogenesis of ChikV and provide insight for the design of candidate antiviral therapeutics.
has issue date	2011-09-08(xsd:dateTime)
bibo:doi	10.1186/1743-422x-8-432
bibo:pmid	21902836
has license	cc-by
sha1sum (hex)	d1d1a8d96a13afa9c54ff26241095b55c0be7ff1
schema:url	https://doi.org/10.1186/1743-422x-8-432
resource representing a document's title	Chikungunya triggers an autophagic process which promotes viral replication
has PubMed Central identifier	PMC3179960
has PubMed identifier	21902836
schema:publication	Virol J
resource representing a document's body	covid:d1d1a8d96a13afa9c54ff26241095b55c0be7ff1#body_text
is schema:about of	named entity 'Background' named entity 'test' named entity 'innate immune' named entity 'experiments' named entity 'mobilize' named entity '2006' named entity 'patients' named entity 'triggers' named entity 'PROCESS' covid:arg/d1d1a8d96a13afa9c54ff26241095b55c0be7ff1 named entity 'CAPACITY' named entity 'ELECTRON MICROSCOPY' named entity 'OUTBREAK' named entity 'STABLE' named entity 'AUTOPHAGY' named entity 'PHYSIOPATHOLOGY' named entity 'TESTED' named entity 'PERCENTAGE' named entity 'SEVERE' named entity 'BECLIN 1' named entity 'VIRAL RNA' named entity 'COMPONENT' named entity 'PROTEIN' named entity 'GENETIC' named entity 'IMPORTANT' named entity 'BLOCKED' named entity 'EITHER' named entity 'FLUORESCENCE MICROSCOPY' named entity 'PERFORMED' named entity 'CELLS' named entity 'ELDERLY PATIENTS' named entity 'METHODS' named entity 'FOUND' named entity 'INDIAN OCEAN' named entity '293' named entity 'MOBILIZE' named entity 'SUPERNATANT' named entity 'GFP' named entity 'MASSIVE' named entity 'RECENTLY' named entity 'TO TEST' named entity 'PHARMACOLOGICAL' named entity 'REPLICATION' named entity 'INNATE IMMUNE RESPONSE' named entity 'RELEASED' named entity 'LC3' named entity 'TIME COURSE' named entity 'DISTRIBUTION' named entity 'EVIDENCE' named entity 'TRIGGERED' named entity 'TRANSCRIPT' named entity 'VIRUS' named entity 'HERE' named entity 'VIRAL REPLICATION' named entity 'PROVIDE' named entity 'CHIKUNGUNYA' named entity 'INVOLVED' named entity 'REACHING' named entity 'CHIKV INFECTION' named entity 'HEK' named entity 'INFANTS' named entity 'CONFIRM' named entity 'RESPONSES' named entity 'DIFFERENT' named entity 'PATHOGENS' named entity 'HOST' named entity 'INHIBITION' named entity 'BACKGROUND' named entity 'ANALYZED' named entity 'PROCESS' named entity 'CHIKUNGUNYA VIRUS' named entity 'HOST DEFENSE' named entity 'IMMUNE' named entity '283'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software