About: Amino acid residues critical for RNA-binding in the N-terminal domain of the nucleocapsid protein are essential determinants for the infectivity of coronavirus in cultured cells

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About: Amino acid residues critical for RNA-binding in the N-terminal domain of the nucleocapsid protein are essential determinants for the infectivity of coronavirus in cultured cells Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Amino acid residues critical for RNA-binding in the N-terminal domain of the nucleocapsid protein are essential determinants for the infectivity of coronavirus in cultured cells
Creator	Lescar, Julien Fan, Hui Fang, Shouguo Liu, D Tan, Yong
Source	Medline; PMC
abstract	The N-terminal domain of the coronavirus nucleocapsid (N) protein adopts a fold resembling a right hand with a flexible, positively charged β-hairpin and a hydrophobic palm. This domain was shown to interact with the genomic RNA for coronavirus infectious bronchitis virus (IBV) and severe acute respiratory syndrome coronavirus (SARS-CoV). Based on its 3D structure, we used site-directed mutagenesis to identify residues essential for the RNA-binding activity of the IBV N protein and viral infectivity. Alanine substitution of either Arg-76 or Tyr-94 in the N-terminal domain of IBV N protein led to a significant decrease in its RNA-binding activity and a total loss of the infectivity of the viral RNA to Vero cells. In contrast, mutation of amino acid Gln-74 to an alanine, which does not affect the binding activity of the N-terminal domain, showed minimal, if any, detrimental effect on the infectivity of IBV. This study thus identifies residues critical for RNA binding on the nucleocapsid surface, and presents biochemical and genetic evidence that directly links the RNA binding capacity of the coronavirus N protein to the viral infectivity in cultured cells. This information would be useful in development of preventive and treatment approaches against coronavirus infection.
has issue date	2006-09-13(xsd:dateTime)
bibo:doi	10.1093/nar/gkl650
bibo:pmid	16971454
has license	cc-by-nc
sha1sum (hex)	d25529180f950874e0b4f62a976959d683a2ceaf
schema:url	https://doi.org/10.1093/nar/gkl650
resource representing a document's title	Amino acid residues critical for RNA-binding in the N-terminal domain of the nucleocapsid protein are essential determinants for the infectivity of coronavirus in cultured cells
has PubMed Central identifier	PMC1635287
has PubMed identifier	16971454
schema:publication	Nucleic Acids Res
resource representing a document's body	covid:d25529180f950874e0b4f62a976959d683a2ceaf#body_text
is schema:about of	named entity 'CULTURED CELLS' named entity 'AMINO ACID' named entity 'CONTRAST' named entity 'SITE-DIRECTED MUTAGENESIS' named entity 'GENOMIC' named entity 'VERO CELLS' named entity 'BIOCHEMICAL' named entity 'TO IDENTIFY' named entity 'VIRAL RNA' named entity 'LED' named entity 'DOMAIN' named entity 'BASED' named entity 'RIGHT HAND' named entity 'ITS' named entity 'BINDING CAPACITY' named entity 'SIGNIFICANT' named entity 'ALANINE' named entity 'ARG-' named entity 'N PROTEIN' named entity 'SURFACE' named entity 'evidence' named entity 'affect' named entity 'N-terminal domain' named entity 'genetic' named entity 'VIRAL' named entity 'TO INTERACT WITH' named entity 'USEFUL' named entity 'BINDING ACTIVITY' named entity 'INFECTIVITY' named entity 'NUCLEOCAPSID PROTEIN' named entity 'AMINO ACID' named entity 'PALM' named entity 'SUBSTITUTION' named entity 'EFFECT' named entity 'DIRECTLY' named entity 'USED' named entity 'POSITIVELY CHARGED' named entity 'ESSENTIAL' named entity 'DOMAIN' named entity 'BINDING' named entity 'DETERMINANTS' named entity 'N-TERMINAL ' named entity 'RNA' named entity 'CORONAVIRUS' named entity 'CRITICAL' named entity 'PROTEIN ' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS' named entity 'GLN-' named entity 'DECREASE' named entity 'RNA BINDING' named entity 'N-TERMINAL ' named entity 'AFFECT' named entity 'LOSS OF' named entity 'CORONAVIRUS INFECTION' named entity 'EVIDENCE' named entity 'RNA' named entity 'APPROACHES' named entity 'LINKS' named entity 'STUDY' named entity 'TOTAL' named entity 'MINIMAL' named entity 'CRITICAL' named entity 'PROTEIN ' named entity 'TREATMENT' named entity 'ESSENTIAL' named entity 'DEVELOPMENT' named entity 'PRESENTS' named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity 'DOES NOT' named entity 'INFECTIVITY' named entity 'CULTURED CELLS' named entity 'GENETIC' named entity 'INFORMATION' named entity 'PREVENTIVE' named entity 'CORONAVIRUS' named entity 'HYDROPHOBIC' named entity 'MUTATION'

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