About: Production of a Monoclonal Antibody Targeting the M Protein of MERS-CoV for Detection of MERS-CoV Using a Synthetic Peptide Epitope Formulated with a CpG–DNA

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About: Production of a Monoclonal Antibody Targeting the M Protein of MERS-CoV for Detection of MERS-CoV Using a Synthetic Peptide Epitope Formulated with a CpG–DNA–Liposome Complex Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Production of a Monoclonal Antibody Targeting the M Protein of MERS-CoV for Detection of MERS-CoV Using a Synthetic Peptide Epitope Formulated with a CpG–DNA–Liposome Complex
Creator	Bae, Joon-Yong Park, Man-Seong Kwon, Hyung-Joo Kwon, Byoung Lee, In Lee, Younghee Su, ·
Source	Medline; PMC
abstract	The Middle East respiratory syndrome-related coronavirus (MERS-CoV) contains four major structural proteins, the spike glycoprotein, nucleocapsid phosphoprotein, membrane (M) glycoprotein and small envelope glycoprotein. The M protein of MERS-CoV has a role in the morphogenesis or assembly of the virus and inhibits type I interferon expression in infected cells. Here, we produced a monoclonal antibody specific against the M protein of MERS-CoV by injecting BALB/c mice with a complex containing the epitope peptide and CpG–DNA encapsulated with a phosphatidyl-β-oleoyl-γ-palmitoyl ethanolamine (DOPE):cholesterol hemisuccinate (CHEMS). The monoclonal antibody was reactive to the epitope peptide of the M protein of MERS-CoV which was confirmed by western blotting and immunoprecipitations. Indirect immunofluorescence assay and confocal image analysis showed that the monoclonal antibody binds specifically to the M protein of MERS-CoV in the virus-infected cells. Further studies using this monoclonal antibody may provide important information on the function of the M protein and its future application in diagnostics.
has issue date	2018-07-02(xsd:dateTime)
bibo:doi	10.1007/s10989-018-9731-8
bibo:pmid	32214929
has license	no-cc
sha1sum (hex)	d2769e9a0a31d4d82ffd7e92d468cc9d10c8fa5e
schema:url	https://doi.org/10.1007/s10989-018-9731-8
resource representing a document's title	Production of a Monoclonal Antibody Targeting the M Protein of MERS-CoV for Detection of MERS-CoV Using a Synthetic Peptide Epitope Formulated with a CpG–DNA–Liposome Complex
has PubMed Central identifier	PMC7087630
has PubMed identifier	32214929
schema:publication	Int J Pept Res Ther
resource representing a document's body	covid:d2769e9a0a31d4d82ffd7e92d468cc9d10c8fa5e#body_text
is schema:about of	named entity 'M protein' named entity 'western blotting' named entity 'cholesterol' named entity 'specific' named entity 'monoclonal antibody' named entity 'Indirect immunofluorescence' named entity 'Complex' named entity 'EPITOPE' named entity 'MERS-COV' named entity 'PRODUCTION' named entity 'IMAGE ANALYSIS' named entity 'INTERFERON ' named entity 'IMMUNOPRECIPITATIONS' named entity 'HEMISUCCINATE' named entity 'PROVIDE' named entity 'IMMUNOFLUORESCENCE ASSAY' named entity 'BINDS' named entity 'VIRUS' named entity 'REACTIVE' named entity 'CONFIRMED BY' named entity 'CONTAINS' named entity 'CONTAINING' named entity 'ASSEMBLY' named entity 'PHOSPHOPROTEIN' named entity 'NUCLEOCAPSID' covid:arg/d2769e9a0a31d4d82ffd7e92d468cc9d10c8fa5e named entity 'ITS' named entity 'MONOCLONAL ANTIBODY' named entity 'ROLE' named entity 'EPITOPE PEPTIDE' named entity 'DETECTION' named entity 'USING' named entity 'COMPLEX' named entity 'DNA' named entity 'LIPOSOME' named entity 'TARGETING' named entity 'MONOCLONAL ANTIBODY' named entity 'SYNTHETIC PEPTIDE' named entity 'EXPRESSION' named entity 'INFECTED' named entity 'BALB' named entity 'OLEOYL' named entity 'MICE' named entity 'FUNCTION' named entity 'SPECIFIC' named entity 'FUTURE' named entity 'INFORMATION' named entity 'USING' named entity 'ETHANOLAMINE' named entity 'COMPLEX' named entity 'SMALL' named entity 'MERS-COV' named entity 'MAJOR' named entity 'MORPHOGENESIS' named entity 'WESTERN BLOTTING' named entity 'STUDIES' named entity 'M PROTEIN' named entity 'PROTEIN ' named entity 'CPG' named entity 'TYPE I INTERFERON' named entity 'DIAGNOSTICS' named entity 'PROTEIN ' named entity 'DNA' named entity 'PHOSPHATIDYL' named entity 'ENVELOPE GLYCOPROTEIN' named entity 'CPG' named entity 'PALMITOYL' named entity 'M PROTEIN' named entity 'INJECTING' named entity 'IMPORTANT' named entity 'HERE' named entity 'APPLICATION' named entity 'MIDDLE EAST RESPIRATORY SYNDROME-RELATED CORONAVIRUS' named entity 'MEMBRANE' named entity 'STRUCTURAL PROTEINS'

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