About: A method of processing nasopharyngeal swabs to enable multiple testing

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A method of processing nasopharyngeal swabs to enable multiple testing
Creator	Johnson, Monika Kurs-Lasky, Marcia Shaikh, Nader Williams, John Cooper, Vaughn Horne, William Lopez, Santiago Marshall, Christopher Martin, Judith
Source	PMC
abstract	OBJECTIVE: To develop a method to perform multiple tests on single nasopharyngeal (NP) swab. STUDY DESIGN: We collected a NP swab on children aged 2–12 years with acute sinusitis and processed it for bacterial culture, viruses, cytokine expression, and 16S ribosomal RNA gene sequencing analysis. During the course of the study, we expand the scope of evaluation to include RNA sequencing, which we accomplished by cutting the tip of the swab. RESULTS: Of the 174 children enrolled, 126 (72.4%) had a positive bacterial culture and 121(69.5%) tested positive for a virus. Cytokine measurement, as judged by the adequate levels of a housekeeping enzyme (GAPDH), appeared successful. From the samples used for 16S ribosomal sequencing we recovered, on average, 16,000 sequences per sample, accounting for a total of 2,646 operational taxonomic units across all samples sequenced. Samples used for RNA sequencing had a mean RNA Integrity number of 6.0. Cutting the tip of the swab did not affect the recovery yield for viruses or bacteria, nor did it affect species richness in microbiome analysis. CONCLUSION: We describe a minimally invasive sample collection protocol that allows for multiple diagnostic and research investigations in young children.
has issue date	2019-07-09(xsd:dateTime)
bibo:doi	10.1038/s41390-019-0498-1
bibo:pmid	31288247
has license	no-cc
sha1sum (hex)	d3a675bfc74c8ebef84c248dca0b8cc05f5b3ce1
schema:url	https://doi.org/10.1038/s41390-019-0498-1
resource representing a document's title	A method of processing nasopharyngeal swabs to enable multiple testing
has PubMed Central identifier	PMC6851467
has PubMed identifier	31288247
schema:publication	Pediatr Res
resource representing a document's body	covid:d3a675bfc74c8ebef84c248dca0b8cc05f5b3ce1#body_text
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