About: Dereplication by High-Performance Liquid Chromatography (HPLC) with Quadrupole-Time-of-Flight Mass Spectroscopy (qTOF-MS) and Antiviral Activities of Phlorotannins from Ecklonia cava

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About: Dereplication by High-Performance Liquid Chromatography (HPLC) with Quadrupole-Time-of-Flight Mass Spectroscopy (qTOF-MS) and Antiviral Activities of Phlorotannins from Ecklonia cava Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Dereplication by High-Performance Liquid Chromatography (HPLC) with Quadrupole-Time-of-Flight Mass Spectroscopy (qTOF-MS) and Antiviral Activities of Phlorotannins from Ecklonia cava
Creator	Kim, Thi Oh, Won Woo, Hyun Cho, Hyo Cho, Tae Doan, Thi Ha, Quy Lee, Ba Pham, Tung Thanh, Ha
Source	PMC
abstract	Ecklonia cava is edible seaweed that is found in Asian countries, such as Japan and Korea; and, its major components include fucoidan and phlorotannins. Phlorotannins that are isolated from E. cava are well-known to have an antioxidant effect and strong antiviral activity against porcine epidemic diarrhea virus (PEDV), which has a high mortality rate in piglets. In this study, the bioactive components were determined based on two different approaches: (i) bio-guided isolation using the antiviral activity against the H1N1 viral strain, which is a representative influenza virus that originates from swine and (ii) high-resolution mass spectrometry-based dereplication, including relative mass defects (RMDs) and HPLC-qTOFMS fragmentation analysis. The EC70 fraction showed the strongest antiviral activity and contained thirteen phlorotannins, which were predicted by dereplication. Ten compounds were directly isolated from E. cava extract and then identified. Moreover, the dereplication method allowed for the discovery of two new phlorotannins. The structures of these two isolated compounds were elucidated using NMR techniques and HPLC-qTOFMS fragmentation analysis. In addition, molecular modelling was applied to determine the absolute configurations of the two new compounds. The antiviral activities of seven major phlorotannins in active fraction were evaluated against two influenza A viral strains (H1N1 and H9N2). Six of the compounds showed moderate to strong effects on both of the viruses and phlorofucofuroeckol A (12), which showed an EC(50) value of 13.48 ± 1.93 μM, is a potential active antiviral component of E. cava.
has issue date	2019-03-04(xsd:dateTime)
bibo:doi	10.3390/md17030149
bibo:pmid	30836593
has license	cc-by
sha1sum (hex)	d3a809d8853624054040f382feb05893b9f6cb7a
schema:url	https://doi.org/10.3390/md17030149
resource representing a document's title	Dereplication by High-Performance Liquid Chromatography (HPLC) with Quadrupole-Time-of-Flight Mass Spectroscopy (qTOF-MS) and Antiviral Activities of Phlorotannins from Ecklonia cava
has PubMed Central identifier	PMC6471242
has PubMed identifier	30836593
schema:publication	Mar Drugs
resource representing a document's body	covid:d3a809d8853624054040f382feb05893b9f6cb7a#body_text
is schema:about of	named entity 'mortality rate' named entity 'fragmentation' named entity 'thirteen' named entity 'fraction' named entity 'fragmentation' named entity 'based' named entity 'strong' named entity 'isolated' named entity 'Antiviral' named entity 'Spectroscopy' named entity 'cava' covid:arg/d3a809d8853624054040f382feb05893b9f6cb7a named entity 'ECKLONIA CAVA' named entity 'VIRAL' named entity 'INCLUDE' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'COMPONENTS' named entity 'ANTIOXIDANT' named entity 'METHOD' named entity 'INFLUENZA VIRUS' named entity 'ACTIVITIES' named entity 'STUDY' named entity 'EDIBLE SEAWEED' named entity 'PHLOROTANNINS' named entity 'EXTRACT' named entity 'NEW' named entity 'FOUND' named entity 'DISCOVERY' named entity 'DIRECTLY' named entity 'ISOLATED' named entity 'PHLOROFUCOFUROECKOL A' named entity 'STRONG' named entity 'IS A' named entity 'FRAGMENTATION' named entity 'JAPAN' named entity 'MAJOR' named entity 'INFLUENZA A' named entity 'SEVEN' named entity 'FUCOIDAN' named entity 'DIFFERENT' named entity 'ANTIVIRAL ACTIVITY' named entity 'NMR' named entity 'HPLC' named entity 'EFFECT' named entity 'BASED' named entity 'ITS' named entity 'SWINE' named entity 'ANTIVIRAL' named entity 'LIQUID CHROMATOGRAPHY ' named entity 'DRUGS' named entity 'MARINE' named entity 'REPRESENTATIVE' named entity 'COMPONENT' named entity 'IDENTIFIED' named entity 'RELATIVE MASS' named entity 'FRACTION' named entity 'USING' named entity 'TECHNIQUES' named entity 'PREDICTED' named entity 'HIGH' named entity 'MORTALITY RATE' named entity 'ANALYSIS' named entity 'DETERMINED' named entity 'THIRTEEN' named entity 'MOLECULAR MODELLING' named entity 'HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY' named entity 'ACTIVITIES' named entity 'QUADRUPOLE' named entity 'H1N1' named entity 'TIME-OF-FLIGHT MASS SPECTROSCOPY' named entity 'KNOWN' named entity 'MODERATE'

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