About: Small Interfering RNA Effectively Inhibits the Expression of SARS Coronavirus Membrane Gene at Two Novel Targeting Sites

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About: Small Interfering RNA Effectively Inhibits the Expression of SARS Coronavirus Membrane Gene at Two Novel Targeting Sites Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Small Interfering RNA Effectively Inhibits the Expression of SARS Coronavirus Membrane Gene at Two Novel Targeting Sites
Creator	Zhang, Yun Liu, Li Wang, Ying Yang, Fan Cao, Ying-Li Wang, Shu-Hui
Source	Medline; PMC
abstract	Small interfering RNA (siRNA) is a class of duplex RNA molecules of 21-25 nt nucleotides in length functioning post-transcriptionally to downregulate targeted gene expression. The membrane (M) protein of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is highly abundant during viral infections and is a critical element for viral assembly. Nucleotide substitution in the viral genome occurs frequently during SARS-CoV infection. In the current study, we analyzed the M gene sequences derived from 15 SARS-CoV isolates and uncovered six nucleotide substitutions among these isolates. Interestingly, these nucleotide substitutions are all located at the 5’ half of the M gene. Based on this information and previous reports, we created two novel siRNAs targeting two unexplored and well conserved regions in the M gene. The effects of these two siRNAs were tested by semi-quantitative RT-PCR and EGFP-M fusion gene expression. The results demonstrated that both siRNAs effectively and specifically blocked the targeted gene expression. Real time quantitative RT-PCR (qRT-PCR) revealed that siRNA targeting the 3’ half of the M gene (si-M2) induced more potent inhibition than that targeting the 5’ half (si-M1). Both si-M1 and si-M2 significantly downregulated M gene mediated upregulation of interferon β expression. Thus, our results indicate that SARS-CoV M gene specific siRNA might function in a sequence-dependent manner.
has issue date	2010-10-18(xsd:dateTime)
bibo:doi	10.3390/molecules15107197
bibo:pmid	20956884
has license	cc-by
sha1sum (hex)	d3f7f0af7fceb8d73489863fca4c5ebb608f6fd4
schema:url	https://doi.org/10.3390/molecules15107197
resource representing a document's title	Small Interfering RNA Effectively Inhibits the Expression of SARS Coronavirus Membrane Gene at Two Novel Targeting Sites
has PubMed Central identifier	PMC6259191
has PubMed identifier	20956884
schema:publication	Molecules
resource representing a document's body	covid:d3f7f0af7fceb8d73489863fca4c5ebb608f6fd4#body_text
is schema:about of	named entity 'ASSOCIATED' named entity 'SEMI-QUANTITATIVE' named entity 'INTERFERON ' named entity 'OUR' named entity 'EXPRESSION' named entity 'MEDIATED' named entity 'REGIONS' named entity 'EGFP' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'FUNCTION' named entity 'SARS-COV' named entity 'TARGETED' named entity 'CORONAVIRUS' named entity 'FREQUENTLY' named entity 'SIRNAS' named entity 'GENE EXPRESSION' named entity 'FUSION GENE' named entity 'QUANTITATIVE RT-PCR' named entity 'CREATED' named entity 'VIRAL INFECTIONS' named entity 'REAL TIME' named entity 'CONSERVED' named entity 'HIGHLY' named entity 'M GENE' named entity 'RNA' named entity 'FUNCTIONING' named entity 'SEQUENCE' named entity 'THESE' named entity 'REVEALED' named entity 'UNCOVERED' named entity 'BASED' named entity 'ABUNDANT' named entity 'POST' named entity 'CRITICAL' named entity 'PREVIOUS REPORTS' named entity 'SITES' named entity 'ANALYZED' named entity 'UPREGULATION' named entity 'VIRAL ASSEMBLY' named entity 'SEQUENCES' named entity 'TARGETING' named entity 'IS A' named entity 'NUCLEOTIDE' named entity 'VIRAL GENOME' named entity 'SPECIFIC' named entity 'SIRNA' named entity 'INHIBITION' named entity 'PCR' named entity 'SMALL INTERFERING RNA' named entity 'NOVEL' named entity 'GENE' named entity 'LENGTH' named entity 'SMALL INTERFERING RNA' named entity 'TARGETING' named entity 'SARS CORONAVIRUS' named entity 'EXPRESSION' named entity 'NUCLEOTIDES' named entity 'HALF' named entity 'NOVEL' named entity 'A CLASS' named entity 'ISOLATES' named entity 'INFORMATION' named entity 'STUDY' named entity 'MOLECULES' named entity 'PROTEIN ' named entity 'DEPENDENT' named entity 'CURRENT' named entity 'DERIVED' named entity 'MEMBRANE' named entity 'DUPLEX' named entity 'MEMBRANE' named entity 'EFFECTS' named entity 'TESTED' named entity 'MOLECULES' named entity 'BLOCKED' named entity 'ELEMENT'

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