About: Autoradiographic detection of IgG and viral antigens

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About: Autoradiographic detection of IgG and viral antigens Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Autoradiographic detection of IgG and viral antigens
Creator	Burks, J Gerdes, J Hileman, L Mcnally, I
Source	Elsevier; Medline; PMC
abstract	Abstract Autoradiographic methods can be used as an alternative to indirect immunofluorescence to detect viral antigen expression or the presence of IgG in tissue sections. Iodinated protein A isolated from Staphylococcus aureus detects an influx of IgG into the central nervous system of mice inoculated with the coronavirus SD. Antispecies antibody that has been iodinated detects coronavirus antigen expression for 24 days post-inoculation while it is only detectable for 10 days by immunofluorescence. A direct comparison of indirect fluorescence and autoradiographic methods indicates that the autoradiographic techniques are considerably more sensitive. This increased sensitivity is sufficient to permit the detection of viral antigen in formalin fixed paraffin embedded tissue sections.
has issue date	1982-10-29(xsd:dateTime)
bibo:doi	10.1016/0022-1759(82)90060-6
bibo:pmid	6294183
has license	els-covid
sha1sum (hex)	d3fbcedc8b7a8b3f10187502b64dd3abaeab56a4
schema:url	https://doi.org/10.1016/0022-1759%2882%2990060-6
resource representing a document's title	Autoradiographic detection of IgG and viral antigens
has PubMed Central identifier	PMC7130982
has PubMed identifier	6294183
schema:publication	Journal of Immunological Methods
resource representing a document's body	covid:d3fbcedc8b7a8b3f10187502b64dd3abaeab56a4#body_text
is schema:about of	named entity 'sections' named entity 'Staphylococcus aureus' named entity 'expression' named entity 'IGG' named entity 'DETECTION' named entity 'DIRECT' named entity 'STAPHYLOCOCCUS AUREUS' named entity 'INOCULATION' named entity 'INCREASED SENSITIVITY' named entity 'LGG' named entity 'ANTIBODY' named entity 'FORMALIN' named entity 'FIXED' named entity 'SENSITIVE' named entity 'IMMUNOFLUORESCENCE' named entity 'FLUORESCENCE' named entity 'INDIRECT IMMUNOFLUORESCENCE' named entity 'CORONAVIRUS' named entity 'DETECTION' named entity 'AUTORADIOGRAPHIC TECHNIQUES' named entity 'CENTRAL NERVOUS SYSTEM' named entity 'ISOLATED' named entity 'VIRAL ANTIGENS' named entity 'PROTEIN A' named entity 'INDIRECT' named entity 'METHODS' named entity 'TO DETECT' named entity 'POST' named entity 'PERMIT' named entity 'PRESENCE OF' named entity 'PARAFFIN EMBEDDED TISSUE' named entity 'EXPRESSION' named entity 'COMPARISON' named entity 'SUFFICIENT' named entity 'VIRAL ANTIGEN' named entity 'ALTERNATIVE' named entity 'DETECTABLE' named entity 'IGG' named entity 'DAYS' named entity 'ANTIGEN' named entity 'TISSUE' named entity 'MICE' named entity 'USED' covid:arg/d3fbcedc8b7a8b3f10187502b64dd3abaeab56a4 named entity 'This' named entity 'indirect immunofluorescence' named entity 'detection' named entity 'immunofluorescence' named entity 'antigen' named entity 'fluorescence' named entity 'fluorescence' named entity 'coronavirus' named entity 'antigen' named entity 'coronavirus' named entity 'formalin' named entity 'antigen' named entity 'Autoradiographic' named entity 'antigen' named entity 'protein' named entity 'calcium sulfate' named entity 'antigen' named entity 'temporal cortex' named entity 'protein' named entity 'protein' named entity 'staphylococcal' named entity 'cryostat' named entity 'antibody' named entity 'temporal cortex' named entity 'liver' named entity 'BSA' named entity 'PBS' named entity 'Bio-Rad' named entity 'phosphate-buffered saline' named entity 'immunofluorescence' named entity 'antigen' named entity 'TCA' named entity 'antibodies' named entity 'focal lesions'

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