About: Evaluation on the Efficacy and Immunogenicity of Recombinant DNA Plasmids Expressing Spike Genes from Porcine Transmissible Gastroenteritis Virus and Porcine Epidemic Diarrhea Virus

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About: Evaluation on the Efficacy and Immunogenicity of Recombinant DNA Plasmids Expressing Spike Genes from Porcine Transmissible Gastroenteritis Virus and Porcine Epidemic Diarrhea Virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Evaluation on the Efficacy and Immunogenicity of Recombinant DNA Plasmids Expressing Spike Genes from Porcine Transmissible Gastroenteritis Virus and Porcine Epidemic Diarrhea Virus
Creator	Li, X Meng, F Ren, Y Sun, X Suo, S
Source	Medline; PMC
abstract	Porcine transmissible gastroenteritis virus (TGEV) and porcine epidemic diarrhea virus (PDEV) can cause severe diarrhea in pigs. Development of effective vaccines against TGEV and PEDV is one of important prevention measures. The spike (S) protein is the surface glycoprotein of TGEV and PEDV, which can induce specific neutralization antibodies and is a candidate antigen for vaccination attempts. In this study, the open reading frames of the TGEV S1 protein and in addition of the S or S1 proteins of PEDV were inserted into the eukaryotic expression vector, pIRES, resulting in recombinant plasmids, pIRES-(TGEV-S1-PEDV-S1) and pIRES-(TGEV-S1-PEDV-S). Subsequently, 6–8 weeks old Kunming mice were inoculated with both DNA plasmids. Lymphocyte proliferation assay, virus neutralization assay, IFN-γ assay and CTL activity assay were performed. TGEV/PEDV specific antibody responses as well as kinetic changes of T lymphocyte subgroups of the immunized mice were analyzed. The results showed that the recombinant DNA plasmids increased the proliferation of T lymphocytes and the number of CD4+ and CD8+ T lymphocyte subgroups. In addition, the DNA vaccines induced a high level of IFN-γ in the immunized mice. The specific CTL activity in the pIRES-(TGEV-S1-PEDV-S) group became significant at 42 days post-immunization. At 35 days post-immunization, the recombinant DNA plasmids bearing full-length S genes of TGEV and PEDV stimulated higher levels of specific antibodies and neutralizing antibodies in immunized mice.
has issue date	2013-03-19(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0057468
bibo:pmid	23526943
has license	cc-by
sha1sum (hex)	d5076ff3eb3e96c862345fa109990f9a7b99b842
schema:url	https://doi.org/10.1371/journal.pone.0057468
resource representing a document's title	Evaluation on the Efficacy and Immunogenicity of Recombinant DNA Plasmids Expressing Spike Genes from Porcine Transmissible Gastroenteritis Virus and Porcine Epidemic Diarrhea Virus
has PubMed Central identifier	PMC3602451
has PubMed identifier	23526943
schema:publication	PLoS One
resource representing a document's body	covid:d5076ff3eb3e96c862345fa109990f9a7b99b842#body_text
is schema:about of	named entity 'neutralization' named entity 'subgroups' named entity 'assay' named entity 'protein' named entity 'Development' named entity 'plasmids' named entity 'mice' named entity 'PROGRAM' named entity 'CHINA' named entity 'EXCELLENT' named entity 'TALENTS' named entity 'GENES' covid:arg/d5076ff3eb3e96c862345fa109990f9a7b99b842 named entity 'SEVERE DIARRHEA' named entity 'PLASMIDS' named entity 'T LYMPHOCYTES' named entity 'ONE OF' named entity 'VACCINATION' named entity 'RESPONSES' named entity 'HIGH LEVEL' named entity 'PIGS' named entity 'EFFECTIVE' named entity 'EUKARYOTIC' named entity 'UNIVERSITY' named entity 'RECOMBINANT DNA' named entity 'CANDIDATE' named entity '28S' named entity 'SUBGROUPS' named entity 'LYMPHOCYTE PROLIFERATION ASSAY' named entity 'NUMBER OF' named entity 'STIMULATED' named entity 'IFN' named entity 'IMPORTANT' named entity 'NEW' named entity 'CHANG' named entity 'RESULTING IN' named entity 'SCHOLAR' named entity 'CENTURY' named entity 'EDUCATION' named entity 'PROGRAMME' named entity 'NEUTRALIZING ANTIBODIES' named entity 'CD4' named entity 'DNA VACCINES' named entity 'ASSAY' named entity 'PROTEINS' named entity 'KUNMING' named entity 'CAUSE' named entity 'LEVELS' named entity 'VIRUS NEUTRALIZATION ASSAY' named entity 'PORCINE TRANSMISSIBLE GASTROENTERITIS VIRUS' named entity 'PERFORMED' named entity 'IS A' named entity 'MICE' named entity 'SPECIFIC ANTIBODY' named entity 'HIGHER' named entity 'CTL' named entity 'T LYMPHOCYTE' named entity 'INSERTED INTO' named entity 'ANTIBODIES' named entity 'INDUCE' named entity 'DEVELOPMENT' named entity 'IMMUNIZED' named entity 'RECOMBINANT' named entity 'GROUP' named entity 'SPECIFIC' named entity 'OLD' named entity 'EXPRESSION VECTOR' named entity 'KINETIC' named entity 'ACTIVITY' named entity 'DAYS' named entity 'INDUCED' named entity 'CD8' named entity 'VACCINES' named entity 'DNA' named entity 'POST' named entity 'ANALYZED' named entity 'CHANGES'

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