About: A classical swine fever virus E2 fusion protein produced in plants elicits a neutralizing humoral immune response in mice and pigs

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A classical swine fever virus E2 fusion protein produced in plants elicits a neutralizing humoral immune response in mice and pigs
Creator	Kim, Dong-Jun, Kim Kang, Lee Kook, Jong Kyungmin, Min Minhee, Park Nam, Hyung Sangmin, Youngmin Seeun, An Sohn, Eun-Ju Sungmin, Gu
Source	PMC
abstract	Classical swine fever (CSF) is one of the most important viral diseases of swine worldwide. Although live or attenuated virus vaccines have been used to control CSFV, it is difficult to distinguish vaccinated pigs from infected pigs; this leads to restrictions on import and export. Subunit vaccines based on the CSFV E2 glycoprotein have been developed using baculovirus or insect cell systems, but some weaknesses remain. Here, we describe production of an E2 recombinant protein using a Nicotiana benthamiana plant expression system. To do this, we took advantage of the ability of the swine Fc domain to increase solubility and stability of the fusion protein and to strengthen immune responses in target animals. N. benthamiana expressed high amounts of pFc2-fused E2 proteins, which were isolated and purified by affinity chromatography to yield a high pure recombinant protein in a cost-effective manner. Native-polyacrylamide gel electrophoresis and size exclusion chromatography confirmed that the pmE2:pFc2 fusion exists as a multimer rather than as a dimer. Injection of recombinant pmE2 protein into mice or piglets generated anti-pmE2 antibodies with efficient neutralizing activity against CSFV. These results suggest that a purified recombinant E2 protein produced in N. benthamiana generates high titers of neutralizing antibodies in vivo; as such, the protein could be developed as a subunit vaccine against CSFV. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10529-020-02892-3) contains supplementary material, which is available to authorized users.
has issue date	2020-04-22(xsd:dateTime)
bibo:doi	10.1007/s10529-020-02892-3
bibo:pmid	32323080
has license	cc-by
sha1sum (hex)	d7055a3cb705bcb0b015693792e0cb04b09ee6ae
schema:url	https://doi.org/10.1007/s10529-020-02892-3
resource representing a document's title	A classical swine fever virus E2 fusion protein produced in plants elicits a neutralizing humoral immune response in mice and pigs
has PubMed Central identifier	PMC7223222
has PubMed identifier	32323080
schema:publication	Biotechnol Lett
resource representing a document's body	covid:d7055a3cb705bcb0b015693792e0cb04b09ee6ae#body_text
is schema:about of	named entity 'weaknesses' named entity 'CSF' named entity 'efficient' named entity 'worldwide' named entity 'generates' named entity 'Classical swine fever' named entity 'pure' named entity 'fusion protein' named entity 'ANTIBODIES' named entity 'POLYACRYLAMIDE GEL ELECTROPHORESIS' named entity 'NEUTRALIZING ANTIBODIES' named entity 'TO INCREASE' named entity 'HERE' named entity 'LEADS' named entity 'CONFIRMED' named entity 'BUT' named entity 'SUBUNIT VACCINES' named entity 'COST' named entity 'YIELD' named entity 'IMPORTANT' named entity 'CELL SYSTEMS' named entity 'SUGGEST' named entity 'ADVANTAGE' named entity 'isolated' named entity 'high' named entity 'recombinant protein' named entity 'recombinant' named entity 'domain' named entity 'Nicotiana benthamiana' named entity 'size exclusion chromatography' named entity 'swine' named entity 'developed' named entity 'purified' named entity 'N. benthamiana' named entity 'N. benthamiana' named entity 'activity' named entity 'piglets' named entity 'Although' named entity 'pigs' named entity 'produced' named entity 'subunit vaccine' named entity 'recombinant protein' named entity 'dimer' named entity 'viral diseases' named entity 'immune responses' named entity 'mice' named entity 'OIE' named entity 'protein' named entity 'vaccination' named entity 'vaccine' named entity 'UTR' named entity 'protein' named entity 'mice' named entity 'vaccination' named entity 'protein' named entity 'cloned' named entity 'adverse effects' named entity 'neutralizing antibodies' named entity 'titer' named entity 'pig' named entity 'vaccine' named entity 'neutralizing antibodies' named entity 'primers' named entity 'recombinant proteins' named entity 'transgenic plant' named entity 'reducing conditions' named entity 'N. benthamiana' named entity 'recombinant' named entity 'N. benthamiana' named entity 'Immunization' named entity 'transgenic plants'

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