About: Promoter structures and differential responses to viral and non-viral inducers of chicken melanoma differentiation-associated gene 5

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About: Promoter structures and differential responses to viral and non-viral inducers of chicken melanoma differentiation-associated gene 5 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Promoter structures and differential responses to viral and non-viral inducers of chicken melanoma differentiation-associated gene 5
Creator	He, Ying Zhang, Ming Chen, Dong-Yang Chen, Mei-Juan Li, Ting-Ting Long, Xie Lu, Ke-Huan Lu, Sheng-Sheng Lu, Yang-Qing Xu, Hui-Yan Yang, Xiao-Gan Zhang, Wen-Xin Zuo, Er-Wei
Source	Elsevier; Medline; PMC
abstract	Abstract Melanoma differentiation-associated gene 5 (MDA5) is a member of the retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs) family and plays a pivotal role in the anti-viral innate immune response. As RIG-I is absent in chickens, MDA5 is hypothesized to be important in detecting viral nucleic acids in the cytoplasm. However, the molecular mechanism of the regulation of chicken MDA5 (chMDA5) expression has yet to be fully elucidated. With this in mind, a ∼2.5kb chMDA5 gene promoter region was examined and PCR amplified to assess its role in immune response. A chMDA5 promoter reporter plasmid (piggybac-MDA5-DsRed) was constructed and transfected into DF-1 cells to establish a Piggybac-MDA5-DsRed cell line. The MDA5 promoter activity was extremely low under basal condition, but was dramatically increased when cells were stimulated with polyinosinic: polycytidylic acid (poly I:C), interferon beta (IFN-β) or Infectious Bursal Disease Virus (IBDV). The DsRed mRNA level represented the promoter activity and was remarkably increased, which matched the expression of endogenous MDA5. However, Infectious Bronchitis Virus (IBV) and Newcastle disease virus (NDV) failed to increase the MDA5 promoter activity and the expression of endogenous MDA5. The results indicated that the promoter and the Piggybac-MDA5-DsRed cell line could be utilized to determine whether a ligand regulates MDA5 expression. For the first time, this study provides a tool for testing chMDA5 expression and regulation.
has issue date	2016-08-31(xsd:dateTime)
bibo:doi	10.1016/j.molimm.2016.06.006
bibo:pmid	27327127
has license	els-covid
sha1sum (hex)	d78cee411daf2fee4fa2dd9ca46b593910d40eba
schema:url	https://doi.org/10.1016/j.molimm.2016.06.006
resource representing a document's title	Promoter structures and differential responses to viral and non-viral inducers of chicken melanoma differentiation-associated gene 5
has PubMed Central identifier	PMC7127162
has PubMed identifier	27327127
schema:publication	Molecular Immunology
resource representing a document's body	covid:d78cee411daf2fee4fa2dd9ca46b593910d40eba#body_text
is schema:about of	named entity 'condition' named entity 'DsRed' named entity 'IBV' named entity 'For' named entity 'amplified' named entity 'cells' named entity 'mind' named entity 'transfected' named entity 'basal' named entity 'CHICKENS' named entity 'STIMULATED' named entity 'COULD BE' named entity 'AMPLIFIED' named entity 'NEWCASTLE DISEASE VIRUS' named entity 'ENDOGENOUS' named entity 'RECEPTORS' named entity 'REPORTER' named entity 'IS A' named entity 'MIND' named entity 'GENE PROMOTER' named entity 'TOOL' named entity 'BUT' named entity 'INTERFERON BETA ' named entity 'LEVEL' named entity 'INCREASED' named entity 'ITS' named entity 'RIG-I' named entity 'CHICKEN' named entity 'CELL LINE' named entity 'LIKE' named entity 'TIME' named entity 'EXAMINED' named entity 'CONDITION' named entity 'INFECTIOUS BURSAL DISEASE VIRUS' named entity 'MEMBER OF' named entity 'CONSTRUCTED' named entity 'INDICATED' named entity 'POLYCYTIDYLIC ACID' named entity 'DETECTING' named entity 'EXPRESSION' named entity 'FAILED' named entity 'LIGAND' named entity 'EXTREMELY' named entity 'PLAYS' named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity 'PROVIDES' named entity 'NUCLEIC ACIDS' named entity 'INNATE IMMUNE RESPONSE' named entity 'ROLE' named entity 'ASSESS' named entity 'FAMILY' named entity 'FULLY' named entity 'MELANOMA DIFFERENTIATION-ASSOCIATED GENE 5' named entity 'DIFFERENTIAL' named entity 'MOLECULAR MECHANISM' named entity 'LOW' named entity 'DETERMINE' named entity 'STRUCTURES' named entity 'RESPONSES' named entity 'CHICKEN' named entity 'REGION' named entity 'REGULATION' named entity 'BC2' named entity 'RETINOIC ACID' named entity 'INDUCIBLE' named entity 'PIGGYBAC' named entity 'VIRAL AND' named entity 'PROMOTER' named entity 'VIRAL'

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