About: Characterization of Neoschoengastia gallinarum from subtropical China by rDNA and identification of two genotypes based on mitochondrial cox1

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About: Characterization of Neoschoengastia gallinarum from subtropical China by rDNA and identification of two genotypes based on mitochondrial cox1 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Characterization of Neoschoengastia gallinarum from subtropical China by rDNA and identification of two genotypes based on mitochondrial cox1
Creator	Weng, Ya-Biao Zhou, Qiang Lin, Rui-Qing Lu, Jun-Peng Qin, Jian-Ping Tan, Zhi-Jian Tao, Jia-Meng Wang, Li-Mei Wang, Zhan-Xin
Source	PMC
abstract	Genetic variations in the 18S ribosomal DNA (18S), 28S ribosomal DNA (28S), second internal transcribed spacer of ribosomal DNA (ITS2), and mitochondrial cytochrome c oxidase subunit 1 (cox1) of Neoschoengastia gallinarum collected from subtropical China were examined. First, a portion of the 18S (p18S), a portion of the 28S (p28S), and the complete ITS2 were separately amplified from individual mites and sequenced. The lengths of the sequences of p18S, p28S, and ITS2 were found to be 1379 bp, 3465~3468 bp, and 200 bp, respectively. The intraspecific sequence variation was 0~0.1% for p28S and 0~1.6% for ITS2, though no variation was observed for p18S, suggesting conservation of rDNA sequences. Second, a portion of the mitochondrial cox1 gene (pcox1) of N. gallinarum was analyzed. The length of the pcox1 sequence is 460 bp, and two distinct groups were observed in N. gallinarum. All pcox1 sequences in group I were identical, and there was only one nucleotide transition observed in group II; however, 7.0~7.2% variations between the two groups were observed, suggesting that two genotypes of N. gallinarum: genotype I and genotype II. Phylogenetic analyses based on pcox1 sequences indicated that N. gallinarum isolates (genotype I or genotype II) clustered into one branch; according to cox1 sequence analysis of Trombiculidae, Walchia hayashii is the closest species. The present study shows that ITS2 rDNA sequence can act as marker for the identification of N. gallinarum samples. Furthermore, analysis of the mitochondrial pcox1 sequence suggests the existence of two genotypes, which has implications for further studies of the ecology and population genetic structures of N. gallinarum.
has issue date	2020-08-22(xsd:dateTime)
bibo:doi	10.1007/s00436-020-06856-4
bibo:pmid	32827102
has license	no-cc
sha1sum (hex)	d807004d7807ab47cdf4a14bf740ec9af684a1b7
schema:url	https://doi.org/10.1007/s00436-020-06856-4
resource representing a document's title	Characterization of Neoschoengastia gallinarum from subtropical China by rDNA and identification of two genotypes based on mitochondrial cox1
has PubMed Central identifier	PMC7442544
has PubMed identifier	32827102
schema:publication	Parasitol Res
resource representing a document's body	covid:d807004d7807ab47cdf4a14bf740ec9af684a1b7#body_text
is schema:about of	named entity 'group I' named entity 'ITS2' named entity 'observed' named entity 'mitochondrial' named entity '18S' named entity 'individual' named entity 'separately' named entity 'length' named entity 'variations' named entity 'GENE' named entity 'ANALYSIS' named entity 'STUDIES' named entity '200' named entity '28S' named entity 'TRANSCRIBED' named entity 'observed' named entity 'based' named entity 'nucleotide' named entity 'subunit' named entity 'existence' named entity 'genotype' named entity 'ribosomal DNA' named entity 'genotype' named entity 'ITS2' named entity 'genotypes' named entity 'studies' named entity 'ecology' named entity 'cox1' named entity 'ribosomal DNA' named entity 'Trombiculidae' named entity 'China' named entity 'ribosomal DNA' named entity 'genotypes' named entity 'genotype' named entity 'population genetic' named entity 'genotype' named entity 'genotype' named entity 'cox1' named entity 'genotypes' named entity 'internal transcribed spacer' named entity 'ITS2' named entity 'Promega' named entity 'Guangdong Province' named entity '5.8S' named entity 'infection' named entity 'mtDNA' named entity 'intraspecific' named entity 'Medina' named entity 'GenBank' named entity 'China' named entity 'ethidium bromide' named entity 'mite' named entity 'NCBI' named entity 'GenBank' named entity 'poultry' named entity 'cox1' named entity 'optical microscopy' named entity 'point mutations' named entity 'Trombiculidae' named entity 'parasite' named entity 'genetic distance' named entity 'Yunfu' named entity 'mitochondrial genes' named entity 'dNTP' named entity 'chicken' named entity 'rDNA' named entity 'GenBank' named entity 'Eukaryotic' named entity 'plasmids' named entity '28S rDNA' named entity 'mitochondrial' named entity 'lateral' named entity 'evolution'

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