About: Development of a molecular-beacon-based multi-allelic real-time RT-PCR assay for the detection of human coronavirus causing severe acute respiratory syndrome (SARS-CoV): a general methodology for detecting rapidly mutating viruses

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About: Development of a molecular-beacon-based multi-allelic real-time RT-PCR assay for the detection of human coronavirus causing severe acute respiratory syndrome (SARS-CoV): a general methodology for detecting rapidly mutating viruses Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Development of a molecular-beacon-based multi-allelic real-time RT-PCR assay for the detection of human coronavirus causing severe acute respiratory syndrome (SARS-CoV): a general methodology for detecting rapidly mutating viruses
Creator	Mazzulli, Tony Butany, Jagdish Poutanen, Susan Asa, Sylvia Demetriou, Victoria Farcas, Gabriella Hadjinicolaou, Andreas Kain, Kevin Willey, Barbara Donald, • Kostrikis, G Leondios, • Low, E
source	Medline; PMC
abstract	Emerging infectious diseases have caused a global effort for development of fast and accurate detection techniques. The rapidly mutating nature of viruses presents a major difficulty, highlighting the need for specific detection of genetically diverse strains. One such infectious agent is SARS-associated coronavirus (SARS-CoV), which emerged in 2003. This study aimed to develop a real-time RT-PCR detection assay specific for SARS-CoV, taking into account its intrinsic polymorphic nature due to genetic drift and recombination and the possibility of continuous and multiple introductions of genetically non-identical strains into the human population, by using mismatch-tolerant molecular beacons designed to specifically detect the SARS-CoV S, E, M and N genes. These were applied in simple, reproducible duplex and multiplex real-time PCR assays on 25 post-mortem samples and constructed RNA controls, and they demonstrated high target detection ability and specificity. This assay can readily be adapted for detection of other emerging and rapidly mutating pathogens.
has issue date	2011-01-11(xsd:dateTime)
bibo:doi	10.1007/s00705-010-0906-7
bibo:pmid	21221674
has license	no-cc
sha1sum (hex)	d8677ff9d31b7d371f20278b2a5aa8c898a49fba
schema:url	https://doi.org/10.1007/s00705-010-0906-7
resource representing a document's title	Development of a molecular-beacon-based multi-allelic real-time RT-PCR assay for the detection of human coronavirus causing severe acute respiratory syndrome (SARS-CoV): a general methodology for detecting rapidly mutating viruses
has PubMed Central identifier	PMC7087183
has PubMed identifier	21221674
schema:publication	Arch Virol
resource representing a document's body	covid:d8677ff9d31b7d371f20278b2a5aa8c898a49fba#body_text
is schema:about of	named entity 'GENERAL' named entity 'CAUSING' named entity 'polymorphic' named entity 'detection' named entity 'designed' named entity 'detection' named entity 'emerged' named entity 'VIRUSES' named entity 'RT-PCR ASSAY' named entity 'MOLECULAR' named entity 'RECOMBINATION' named entity 'DIVERSE' named entity 'INTRINSIC' named entity 'DUPLEX' named entity 'DETECTION' named entity 'CONSTRUCTED' named entity 'POLYMORPHIC' named entity 'ITS' named entity 'CAUSED' named entity 'SAMPLES' named entity 'INFECTIOUS AGENT' named entity 'TOLERANT' named entity 'SARS-ASSOCIATED CORONAVIRUS' named entity 'SPECIFIC' named entity 'ADAPTED' covid:arg/d8677ff9d31b7d371f20278b2a5aa8c898a49fba named entity 'METHODOLOGY' named entity 'USING' named entity 'GENETIC DRIFT' named entity 'EFFORT' named entity 'RNA' named entity 'HIGH' named entity 'THESE' named entity 'SARS-COV' named entity 'ASSAY' named entity 'APPLIED' named entity 'IDENTICAL' named entity 'POSSIBILITY OF' named entity 'ACCURATE' named entity 'PRESENTS' named entity 'HAVE' named entity 'TAKING' named entity 'NEED FOR' named entity 'HIGHLIGHTING' named entity 'RAPIDLY' named entity 'REAL-TIME PCR ' named entity 'DEMONSTRATED' named entity 'REAL-TIME RT-PCR' named entity 'RAPIDLY' named entity 'DEVELOPMENT' named entity 'MULTIPLEX REAL-TIME PCR' named entity 'ACCOUNT' named entity 'REAL-TIME RT-PCR' named entity 'CONTINUOUS' named entity 'MISMATCH' named entity 'MAJOR DIFFICULTY' named entity 'BASED' named entity 'DETECTING' named entity 'HUMAN CORONAVIRUS' named entity 'DETECTION' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'BEACON' named entity 'A MAJOR' named entity 'GENES' named entity 'FAST' named entity 'MULTIPLE' named entity 'EMERGING INFECTIOUS DISEASES' named entity 'PATHOGENS' named entity 'POST-MORTEM'

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