About: Sequence-specific detection of single-stranded DNA with a gold nanoparticle-protein nanopore approach

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About: Sequence-specific detection of single-stranded DNA with a gold nanoparticle-protein nanopore approach Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Sequence-specific detection of single-stranded DNA with a gold nanoparticle-protein nanopore approach
Creator	Asandei, Alina Bucataru, Ioana Dragomir, Isabela Luchian, Tudor Mereuta, Loredana Park, Jonggwan Park, Yoonkyung Seo, Chang
source	Medline; PMC
abstract	Fast, cheap and easy to use nucleic acids detection methods are crucial to mitigate adverse impacts caused by various pathogens, and are essential in forensic investigations, food safety monitoring or evolution of infectious diseases. We report here a method based on the α-hemolysin (α-HL) nanopore, working in conjunction to unmodified citrate anion-coated gold nanoparticles (AuNPs), to detect nanomolar concentrations of short single-stranded DNA sequences (ssDNA). The core idea was to use charge neutral peptide nucleic acids (PNA) as hybridization probe for complementary target ssDNAs, and monitor at the single-particle level the PNA-induced aggregation propensity AuNPs during PNA–DNA duplexes formation, by recording ionic current blockades signature of AuNP–α-HL interactions. This approach offers advantages including: (1) a simple to operate platform, producing clear-cut readout signals based on distinct size differences of PNA-induced AuNPs aggregates, in relation to the presence in solution of complementary ssDNAs to the PNA fragments (2) sensitive and selective detection of target ssDNAs (3) specific ssDNA detection in the presence of interference DNA, without sample labeling or signal amplification. The powerful synergy of protein nanopore-based nanoparticle detection and specific PNA–DNA hybridization introduces a new strategy for nucleic acids biosensing with short detection time and label-free operation.
has issue date	2020-07-09(xsd:dateTime)
bibo:doi	10.1038/s41598-020-68258-x
bibo:pmid	32647249
has license	cc-by
sha1sum (hex)	d8fc56afbf92f3b590d5d95e0e6be7e5bbdae859
schema:url	https://doi.org/10.1038/s41598-020-68258-x
resource representing a document's title	Sequence-specific detection of single-stranded DNA with a gold nanoparticle-protein nanopore approach
has PubMed Central identifier	PMC7347621
has PubMed identifier	32647249
schema:publication	Sci Rep
resource representing a document's body	covid:d8fc56afbf92f3b590d5d95e0e6be7e5bbdae859#body_text
is schema:about of	named entity 'easy' named entity 'relation' named entity 'food safety' named entity 'detection' named entity 'citrate' named entity 'pathogens' named entity 'nucleic acids' named entity 'Fast' named entity 'level' named entity 'SINGLE-STRANDED DNA' named entity 'IDEA' named entity 'FOOD SAFETY' named entity 'NUCLEIC ACIDS' named entity 'AUNP' named entity 'SINGLE' named entity 'PLATFORM' named entity 'STRATEGY' named entity 'RECORDING' named entity 'AGGREGATION' named entity 'HEMOLYSIN' named entity 'IMPACTS' named entity 'TIME' named entity 'ESSENTIAL' named entity 'EVOLUTION' named entity 'TO DETECT' named entity 'NEUTRAL' named entity 'FRAGMENTS' named entity 'PATHOGENS' named entity 'core' named entity 'evolution' named entity 'adverse' named entity 'clear-cut' named entity 'sequences' named entity 'protein' named entity 'caused' named entity 'biosensing' named entity 'PNA' named entity 'anion' named entity 'nanopore' named entity 'nanomolar' named entity 'biosensing' named entity 'DNA sequences' named entity 'ionic current' named entity 'evolution' named entity 'gold nanoparticles' named entity 'DNA hybridization' named entity 'protein' named entity 'nucleic acids' named entity 'PNA' named entity 'single-stranded' named entity 'citrate' named entity 'ssDNA' named entity 'simple' named entity 'nanopore' named entity 'hybridization' named entity 'adsorption' named entity 'absorbance' named entity 'electrolyte' named entity 'absorption spectra' named entity 'sampling frequency' named entity 'hybridization' named entity 'dissociation' named entity 'negative charge' named entity 'Germany' named entity 'infection' named entity 'dissociation' named entity 'biomarker' named entity '16-bit' named entity 'Tris' named entity 'ensuing blockade' named entity 'nucleic acid' named entity 'pathogen' named entity 'biomarkers' named entity 'Sigma-Aldrich'

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