About: Identification and characterization of linear B cell epitopes on the nucleocapsid protein of porcine epidemic diarrhea virus using monoclonal antibodies

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Identification and characterization of linear B cell epitopes on the nucleocapsid protein of porcine epidemic diarrhea virus using monoclonal antibodies Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Identification and characterization of linear B cell epitopes on the nucleocapsid protein of porcine epidemic diarrhea virus using monoclonal antibodies
Creator	Bai, Juan Jiang, Ping Li, Yufeng Shi, Xiaoli Fang, Linlin Liu, Qianyu Lu, Qianqian Wang, Xianwei Zhan, Jing
Source	Elsevier; Medline; PMC
abstract	Abstract The nucleocapsid (N) protein of porcine epidemic diarrhea virus (PEDV), the most important pathogen causing severe diarrhea in piglets, is a highly conserved structural protein. In this study, 5 monoclonal antibodies (McAbs) against the PEDV N-protein were prepared and identified. Three new epitopes, 56QIRWRMRRGERI67, 318GYAQIASLAPNVAALLFGGNVA VRE342 and 398HEEAIYDDV406, were firstly identified in the viral N-protein, by using McAbs 3F10, 6A11, and 1C9. The epitope 398HEEAIYDDV406 was deleted in SH strain (isolated by our lab) and different between CV777 and YZ strain (isolated by our lab). To study the characters of this epitope, four peptides were synthesized according to the sequence of SH and CV777 and used in the study. The result showed that the 398th amino acid maybe an important amino acid of the epitope. Biological information analysis showed that the three B cell linear epitopes are highly conserved among different PEDV isolates. In addition, McAb 1C9, which attached to the epitope 398HEEAIYDDV406, showed variant reactivity with PEDV CV777, SH, YZ and MS strains. McAb 1C9 reacted with PEDV strains CV777 and YZ, but not with SH which had a deletion from 399 to 410 amino acids in N-protein (No. MK841494). Among the three McAbs, 6A11, 3F10 and 1C9, only 6A11 reacted with porcine transmissible gastroenteritis virus (TGEV) in immunofluorescence assay, therefore the other two could be used to distinguish TGEV and PEDV. These mAbs and their defined epitopes may provide useful tool for the study of the PEDV N-protein structure and function, and facilitate the development of diagnostic methods for PEDV.
has issue date	2020-05-31(xsd:dateTime)
bibo:doi	10.1016/j.virusres.2020.197912
bibo:pmid	32142743
has license	els-covid
sha1sum (hex)	d92227b68c6a9bd4f85004578c72b7f3e19f7a21
schema:url	https://doi.org/10.1016/j.virusres.2020.197912
resource representing a document's title	Identification and characterization of linear B cell epitopes on the nucleocapsid protein of porcine epidemic diarrhea virus using monoclonal antibodies
has PubMed Central identifier	PMC7114562
has PubMed identifier	32142743
schema:publication	Virus Research
resource representing a document's body	covid:d92227b68c6a9bd4f85004578c72b7f3e19f7a21#body_text
is schema:about of	named entity 'function' named entity 'provide' named entity 'monoclonal antibodies' named entity 'prepared' named entity 'epitope' named entity 'peptides' named entity 'The' named entity 'highly' named entity 'transmissible' named entity 'epitope' named entity 'reacted' named entity 'porcine' named entity 'strains' named entity 'lab' named entity 'tool' named entity 'reactivity' named entity 'study' named entity 'monoclonal antibodies' named entity 'structural protein' named entity 'epitopes' named entity 'nucleocapsid' named entity 'diagnostic methods' named entity 'nucleocapsid' named entity 'amino acids' named entity 'nucleocapsid' named entity 'protein' named entity 'acid' named entity 'antibody' named entity 'protein' named entity '5.0' named entity 'PEDV' named entity 'MscI' named entity 'antigen' named entity 'immunofluorescence assay' named entity 'PEDV' named entity 'PEDV' named entity 'epitope' named entity 'eukaryotic cells' named entity 'monoclonal antibody' named entity 'gene' named entity 'spike protein' named entity 'Epitope mapping' named entity 'polypeptides' named entity 'UTR' named entity 'antigenicity' named entity 'TGEV' named entity 'PEDV' named entity 'PEDV' named entity 'myeloma' named entity 'epitopes' named entity 'clinical diagnostic' named entity 'protein' named entity 'amino acid' named entity 'epitopes' named entity 'antigen' named entity 'amino acid' named entity 'cDNA' named entity 'IgG' named entity 'gene deletion' named entity 'epitopes' named entity 'immunization' named entity 'protein' named entity 'mice' named entity 'subunit vaccines' named entity '100 %' named entity 'PROTEAN' named entity 'BALB/c' named entity 'peptide' named entity 'vector' named entity 'PEDV' named entity 'structural proteins' named entity 'serum' named entity '37°C'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software