About: Lactate dehydrogenase-elevating virus (LDV): subgenomic mRNAs, mRNA leader and comparison of 3'-terminal sequences of two LDV isolates

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Lactate dehydrogenase-elevating virus (LDV): subgenomic mRNAs, mRNA leader and comparison of 3'-terminal sequences of two LDV isolates Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Lactate dehydrogenase-elevating virus (LDV): subgenomic mRNAs, mRNA leader and comparison of 3'-terminal sequences of two LDV isolates
Creator	Chen, Zongyu Kuo, Lili Faaberg, Kay Plagemann, Peter Rowland, Raymond
Source	Elsevier; Medline; PMC
abstract	Abstract The 3'-terminal 1314 nucleotides of the genome of one isolate of lactate dehydrogenase-elevating virus, LDV-P, has been derived by sequence analyses of cDNAs from several genomic libraries and compared to that of another LDV isolate, LDV-C (Godeny et al. (1990) Virol. 177, 768–771). The 3'-non-coding segment of 80 nucleotides of the two LDV genomes is identical, whereas marked, but varying nucleotide and amino acid divergence is apparent in the three upstream overlapping open reading frames (ORF). The third ORF from the 3'-end exhibits only 82% nucleotide and 90% amino acid identity, whereas the 3'-terminal ORF, which encodes the nucleocapsid protein, exhibits approximately 99% amino acid identity. The second 3'-terminal ORF encodes an 18.8 kDa protein which lacks N-glycosylation sites but possesses 2 or 3 potential transmembrane helices in the N-terminal half of the molecule. A similar membrane organization is observed for the corresponding protein of equine arteritis virus and the M protein of mouse hepatitis virus. The sequence analyses combined with Northern hybridization analyses of RNA from LDV-infected macrophages and spleens of LDV-infected mice indicate that the three ORFs encoded by the 3'-terminal end of the LDV genome are expressed via the three smallest mRNAs (mRNAs 6–8) of the seven subgenomic mRNAs of LDV (mRNAs 2–8), which range in size from about 0.8 to 3.6 kb. All mRNAs have been shown to carry poly(A)-tracts and a common leader sequence. The seven mRNAs were produced in infected macrophage cultures concomitantly with genomic LDV RNA. Maximum LDV RNA synthesis was observed between 6 and 8 h post-infection. The same seven subgenomic mRNAs were detected in macrophages infected with three different isolates of LDV, but different relative amounts of some of the mRNAs were produced. The relative proportions of molecules of mRNAs 1–8 present in 6 h LDV-P-infected macrophages were about 13, 5, 5, 8, 6, 11, 11 and 27% of the total, respectively.
has issue date	1992-04-30(xsd:dateTime)
bibo:doi	10.1016/0168-1702(92)90067-j
bibo:pmid	1604932
has license	els-covid
sha1sum (hex)	d9477a424ff239d8189bde0ed24562d7a12a229a
schema:url	https://doi.org/10.1016/0168-1702%2892%2990067-j
resource representing a document's title	Lactate dehydrogenase-elevating virus (LDV): subgenomic mRNAs, mRNA leader and comparison of 3'-terminal sequences of two LDV isolates
has PubMed Central identifier	PMC7133880
has PubMed identifier	1604932
schema:publication	Virus Research
resource representing a document's body	covid:d9477a424ff239d8189bde0ed24562d7a12a229a#body_text
is schema:about of	named entity 'expressed' named entity 'encoded' named entity 'Lactate' named entity 'TERMINAL' named entity 'ORF' named entity 'LACKS' named entity 'DERIVED' named entity 'HALF' named entity 'BUT' named entity 'SEVEN' named entity '90%' named entity 'MOUSE HEPATITIS VIRUS' named entity 'NUCLEOCAPSID PROTEIN' named entity 'EQUINE ARTERITIS VIRUS' named entity 'SIZE' named entity 'CODING' named entity 'COMBINED' named entity 'SEQUENCES' named entity 'END' named entity 'PROTEIN ' named entity 'NORTHERN HYBRIDIZATION' named entity 'MICE' named entity 'TRANSMEMBRANE' named entity 'LACTATE DEHYDROGENASE-ELEVATING VIRUS' named entity 'MRNA' named entity 'RANGE' named entity 'N-GLYCOSYLATION' named entity 'COMPARISON' named entity 'OBSERVED' named entity 'APPARENT' named entity 'POTENTIAL' named entity 'NON-' named entity 'COMPARED' named entity 'SMALLEST' named entity 'POSSESSES' named entity 'MARKED' named entity 'SIMILAR' named entity '768' named entity 'AMINO ACID' named entity '0.8' named entity 'ISOLATE' named entity 'RNA' named entity 'IDENTITY' named entity 'IDENTICAL' named entity 'SEGMENT' named entity 'MRNAS' named entity 'UPSTREAM' named entity 'VIROL' named entity 'EXHIBITS' named entity 'NUCLEOTIDES' named entity 'NUCLEOTIDE' named entity 'OVERLAPPING' named entity 'MRNAS' named entity 'GENOME' named entity 'INFECTED' named entity 'APPROXIMATELY' named entity 'N-TERMINAL ' named entity 'EXPRESSED' named entity 'OPEN READING FRAMES' named entity 'ENCODED' named entity 'HELICES' named entity 'MEMBRANE ORGANIZATION' named entity 'MOLECULE' named entity 'VARYING' named entity 'ISOLATES' named entity 'GENOMES' named entity 'GENOMIC LIBRARIES' named entity 'MACROPHAGES' named entity 'SITES' named entity 'KDA' named entity 'DIVERGENCE' named entity 'TERMINAL' named entity 'SPLEENS' named entity 'LACTATE DEHYDROGENASE-ELEVATING VIRUS' named entity 'M PROTEIN' named entity 'SECOND' named entity 'SEQUENCE'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software